Statistical evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins.
Int J Biol Macromol. 2023 Apr 01; 233:123470.IJ

Abstract

This research studies in detail four different assays, namely DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), FRAP (ferric ion reducing antioxidant potential) and FC (Folin-Ciocalteu), to determine the antioxidant capacity of standard substances as well as 50 organosolv lignins, and two kraft lignins. The coefficient of variation was determined for each method and was lowest for ABTS and highest for DPPH. The best correlation was found for FRAP and FC, which both rely on a single electron transfer mechanism. A good correlation between ABTS, FRAP and FC, respectively, could be observed, even though ABTS relies on a more complex reaction mechanism. The DPPH assay merely correlates with the others, implying that it reflects different antioxidative attributes due to a different reaction mechanism. Lignins obtained from paulownia and silphium have been investigated for the first time regarding their antioxidant capacity. Paulownia lignin is in the same range as beech wood lignin, while silphium lignin resembles wheat straw lignin. Miscanthus lignin is an exception from the grass lignins and possesses a significantly higher antioxidant capacity. All lignins possess a good antioxidant capacity and thus are promising candidates for various applications, e. g. as additives in food packaging or for biomedical purposes.

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Authors+Show Affiliations

Rumpf J

Department of Natural Sciences, Bonn-Rhein-Sieg University of Applied Sciences, von-Liebig-Strasse 20, D-53359 Rheinbach, Germany; Agricultural Faculty, University of Bonn, Meckenheimer Allee 174, D-53115 Bonn, Germany. Electronic address: jessica.rumpf@h-brs.de.

Burger R

Department of Natural Sciences, Bonn-Rhein-Sieg University of Applied Sciences, von-Liebig-Strasse 20, D-53359 Rheinbach, Germany. Electronic address: rene.burger@h-brs.de.

Schulze M

MeSH

AntioxidantsLigninPlant ExtractsPoaceae

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36736974

Citation

Rumpf, Jessica, et al. "Statistical Evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu Assays to Assess the Antioxidant Capacity of Lignins." International Journal of Biological Macromolecules, vol. 233, 2023, p. 123470.

Rumpf J, Burger R, Schulze M. Statistical evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins. Int J Biol Macromol. 2023;233:123470.

Rumpf, J., Burger, R., & Schulze, M. (2023). Statistical evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins. International Journal of Biological Macromolecules, 233, 123470. https://doi.org/10.1016/j.ijbiomac.2023.123470

Rumpf J, Burger R, Schulze M. Statistical Evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu Assays to Assess the Antioxidant Capacity of Lignins. Int J Biol Macromol. 2023 Apr 1;233:123470. PubMed PMID: 36736974.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Statistical evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins. AU - Rumpf,Jessica, AU - Burger,René, AU - Schulze,Margit, Y1 - 2023/02/02/ PY - 2022/12/03/received PY - 2023/01/17/revised PY - 2023/01/25/accepted PY - 2023/2/4/pubmed PY - 2023/2/4/medline PY - 2023/2/3/entrez KW - Antioxidant capacity KW - Assay reproducibility KW - Lignin SP - 123470 EP - 123470 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 233 N2 - This research studies in detail four different assays, namely DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), FRAP (ferric ion reducing antioxidant potential) and FC (Folin-Ciocalteu), to determine the antioxidant capacity of standard substances as well as 50 organosolv lignins, and two kraft lignins. The coefficient of variation was determined for each method and was lowest for ABTS and highest for DPPH. The best correlation was found for FRAP and FC, which both rely on a single electron transfer mechanism. A good correlation between ABTS, FRAP and FC, respectively, could be observed, even though ABTS relies on a more complex reaction mechanism. The DPPH assay merely correlates with the others, implying that it reflects different antioxidative attributes due to a different reaction mechanism. Lignins obtained from paulownia and silphium have been investigated for the first time regarding their antioxidant capacity. Paulownia lignin is in the same range as beech wood lignin, while silphium lignin resembles wheat straw lignin. Miscanthus lignin is an exception from the grass lignins and possesses a significantly higher antioxidant capacity. All lignins possess a good antioxidant capacity and thus are promising candidates for various applications, e. g. as additives in food packaging or for biomedical purposes. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/36736974/Statistical_evaluation_of_DPPH_ABTS_FRAP_and_Folin_Ciocalteu_assays_to_assess_the_antioxidant_capacity_of_lignins_ DB - PRIME DP - Unbound Medicine ER -

Tags

Statistical evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins.Int J Biol Macromol. 2023 Apr 01; 233:123470.IJ