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Effect of andrographolide and deep eutectic solvent extracts of Andrographis paniculata on human coronavirus organ culture 43 (HCoV-OC43).
Phytomedicine. 2023 Apr; 112:154708.P

Abstract

BACKGROUND

Andrographis paniculata (Burm. f.) Nees has demonstrated potential for treating infections caused by coronaviruses. However, no antiviral activity of andrographolide or A. paniculata extracts against human coronavirus organ culture 43 (HCoV-OC43) has been reported.

PURPOSE

This study aimed to evaluate the anti-HCoV-OC43 effect of andrographolide and A. paniculata as well as the correlation between andrographolide concentration and the anti-HCoV-OC43 activity of A. paniculata extracts.

METHODS

This study evaluated and compared the in vitro anti-HCoV-OC43 activities of various A. paniculata extracts and andrographolide. To obtain A. paniculata extracts with different concentrations of andrographolide and its components, methanol and deep eutectic solvents (DES) were used to extract the aerial parts of A. paniculata. Andrographolide content was determined using UV-HPLC, and antiviral activity was assessed in HCT-8 colon cells.

RESULTS

The methanol and five acidic DES (containing malic acid or citric acid) extracts of A. paniculata exerted anti-HCoV-OC43 activity. Antiviral activity had a moderately strong positive linear relationship (r = 0.7938) with andrographolide content. Although the methanol extract contained the highest andrographolide content (2.34 mg/ml), its anti-HCoV-OC43 activity was lower than that of the DES extracts containing lower andrographolide concentrations (0.92-1.46 mg/ml).

CONCLUSION

Methanol and the five acidic DES extracts of A. paniculata exhibited anti-HCoV-OC43 activity. However, the in vitro antiviral activity of A. paniculata extracts did not have a very strong positive linear relationship (r < 0.8) with andrographolide concentration in the extract. As a result, when comparing A. paniculata extracts, the anti-HCoV-OC43 test could provide a different result from the andrographolide concentration determination.

Links

Authors+Show Affiliations

Center for Vaccine Development, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.

Ruangdachsuwan S

Center for Vaccine Development, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.

Center for Vaccine Development, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.

Center for Vaccine Development, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.

Punyahathaikul S

Center for Vaccine Development, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.

Center for Vaccine Development, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.

Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand.

Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand. Electronic address: tharita.kit@mahidol.edu.

MeSH

HumansPlant ExtractsSolventsAndrographis paniculataDeep Eutectic SolventsMethanolOrgan Culture TechniquesAndrographisDiterpenesCoronavirus

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36805485

Citation

Komaikul, Jukrapun, et al. "Effect of Andrographolide and Deep Eutectic Solvent Extracts of Andrographis Paniculata On Human Coronavirus Organ Culture 43 (HCoV-OC43)." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 112, 2023, p. 154708.

Komaikul J, Ruangdachsuwan S, Wanlayaporn D, et al. Effect of andrographolide and deep eutectic solvent extracts of Andrographis paniculata on human coronavirus organ culture 43 (HCoV-OC43). Phytomedicine. 2023;112:154708.

Komaikul, J., Ruangdachsuwan, S., Wanlayaporn, D., Palabodeewat, S., Punyahathaikul, S., Churod, T., Choonong, R., & Kitisripanya, T. (2023). Effect of andrographolide and deep eutectic solvent extracts of Andrographis paniculata on human coronavirus organ culture 43 (HCoV-OC43). Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 112, 154708. https://doi.org/10.1016/j.phymed.2023.154708

Komaikul J, et al. Effect of Andrographolide and Deep Eutectic Solvent Extracts of Andrographis Paniculata On Human Coronavirus Organ Culture 43 (HCoV-OC43). Phytomedicine. 2023;112:154708. PubMed PMID: 36805485.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Effect of andrographolide and deep eutectic solvent extracts of Andrographis paniculata on human coronavirus organ culture 43 (HCoV-OC43). AU - Komaikul,Jukrapun, AU - Ruangdachsuwan,Sasiporn, AU - Wanlayaporn,Duangnapa, AU - Palabodeewat,Somnuek, AU - Punyahathaikul,Surat, AU - Churod,Theeraporn, AU - Choonong,Rattanathorn, AU - Kitisripanya,Tharita, Y1 - 2023/02/08/ PY - 2022/11/13/received PY - 2023/01/19/revised PY - 2023/02/05/accepted PY - 2023/2/23/pubmed PY - 2023/2/23/medline PY - 2023/2/22/entrez KW - Andrographis KW - Antiviral activity KW - Coronavirus KW - Deep eutectic solvent KW - Herbal medicine KW - Plant extract SP - 154708 EP - 154708 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 112 N2 - BACKGROUND: Andrographis paniculata (Burm. f.) Nees has demonstrated potential for treating infections caused by coronaviruses. However, no antiviral activity of andrographolide or A. paniculata extracts against human coronavirus organ culture 43 (HCoV-OC43) has been reported. PURPOSE: This study aimed to evaluate the anti-HCoV-OC43 effect of andrographolide and A. paniculata as well as the correlation between andrographolide concentration and the anti-HCoV-OC43 activity of A. paniculata extracts. METHODS: This study evaluated and compared the in vitro anti-HCoV-OC43 activities of various A. paniculata extracts and andrographolide. To obtain A. paniculata extracts with different concentrations of andrographolide and its components, methanol and deep eutectic solvents (DES) were used to extract the aerial parts of A. paniculata. Andrographolide content was determined using UV-HPLC, and antiviral activity was assessed in HCT-8 colon cells. RESULTS: The methanol and five acidic DES (containing malic acid or citric acid) extracts of A. paniculata exerted anti-HCoV-OC43 activity. Antiviral activity had a moderately strong positive linear relationship (r = 0.7938) with andrographolide content. Although the methanol extract contained the highest andrographolide content (2.34 mg/ml), its anti-HCoV-OC43 activity was lower than that of the DES extracts containing lower andrographolide concentrations (0.92-1.46 mg/ml). CONCLUSION: Methanol and the five acidic DES extracts of A. paniculata exhibited anti-HCoV-OC43 activity. However, the in vitro antiviral activity of A. paniculata extracts did not have a very strong positive linear relationship (r < 0.8) with andrographolide concentration in the extract. As a result, when comparing A. paniculata extracts, the anti-HCoV-OC43 test could provide a different result from the andrographolide concentration determination. SN - 1618-095X UR - https://www.unboundmedicine.com/medline/citation/36805485/Effect_of_andrographolide_and_deep_eutectic_solvent_extracts_of_Andrographis_paniculata_on_human_coronavirus_organ_culture_43__HCoV_OC43__ DB - PRIME DP - Unbound Medicine ER -