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Genome sequencing of Sitopsis species provides insights into their contribution to the B subgenome of bread wheat.
Plant Commun. 2023 07 10; 4(4):100567.PC

Abstract

Wheat (Triticum aestivum, BBAADD) is an allohexaploid species that originated from two polyploidization events. The progenitors of the A and D subgenomes have been identified as Triticum urartu and Aegilops tauschii, respectively. Current research suggests that Aegilops speltoides is the closest but not the direct ancestor of the B subgenome. However, whether Ae. speltoides has contributed genomically to the wheat B subgenome and which chromosome regions are conserved between Ae. speltoides and the B subgenome remain unclear. Here, we assembled a high-quality reference genome for Ae. speltoides, resequenced 53 accessions from seven species (Aegilops bicornis, Aegilops longissima, Aegilops searsii, Aegilops sharonensis, Ae. speltoides, Aegilops mutica [syn. Amblyopyrum muticum], and Triticum dicoccoides) and revealed their genomic contributions to the wheat B subgenome. Our results showed that centromeric regions were particularly conserved between Aegilops and Triticum and revealed 0.17 Gb of conserved blocks between Ae. speltoides and the B subgenome. We classified five groups of conserved and non-conserved genes between Aegilops and Triticum, revealing their biological characteristics, differentiation in gene expression patterns, and collinear relationships between Ae. speltoides and the wheat B subgenome. We also identified gene families that expanded in Ae. speltoides during its evolution and 789 genes specific to Ae. speltoides. These genes can serve as genetic resources for improvement of adaptability to biotic and abiotic stress. The newly constructed reference genome and large-scale resequencing data for Sitopsis species will provide a valuable genomic resource for wheat genetic improvement and genomic studies.

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Authors+Show Affiliations

Yang Y
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Cui L
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Lu Z
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: luzefu@caas.cn.
Li G
Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
Yang Z
Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
Zhao G
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Kong C
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Li D
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Chen Y
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Xie Z
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Chen Z
Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China.
Zhang L
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Xia C
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Liu X
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: liuxu03@caas.cn.
Jia J
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: jiajizeng@caas.cn.
Kong X
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: kongxiuying@caas.cn.

MeSH

TriticumBreadGenome, PlantChromosome MappingPoaceae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

36855304

Citation

Yang, Yuxin, et al. "Genome Sequencing of Sitopsis Species Provides Insights Into Their Contribution to the B Subgenome of Bread Wheat." Plant Communications, vol. 4, no. 4, 2023, p. 100567.
Yang Y, Cui L, Lu Z, et al. Genome sequencing of Sitopsis species provides insights into their contribution to the B subgenome of bread wheat. Plant Commun. 2023;4(4):100567.
Yang, Y., Cui, L., Lu, Z., Li, G., Yang, Z., Zhao, G., Kong, C., Li, D., Chen, Y., Xie, Z., Chen, Z., Zhang, L., Xia, C., Liu, X., Jia, J., & Kong, X. (2023). Genome sequencing of Sitopsis species provides insights into their contribution to the B subgenome of bread wheat. Plant Communications, 4(4), 100567. https://doi.org/10.1016/j.xplc.2023.100567
Yang Y, et al. Genome Sequencing of Sitopsis Species Provides Insights Into Their Contribution to the B Subgenome of Bread Wheat. Plant Commun. 2023 07 10;4(4):100567. PubMed PMID: 36855304.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome sequencing of Sitopsis species provides insights into their contribution to the B subgenome of bread wheat. AU - Yang,Yuxin, AU - Cui,Licao, AU - Lu,Zefu, AU - Li,Guangrong, AU - Yang,Zujun, AU - Zhao,Guangyao, AU - Kong,Chuizheng, AU - Li,Danping, AU - Chen,Yaoyu, AU - Xie,Zhencheng, AU - Chen,Zhongxu, AU - Zhang,Lichao, AU - Xia,Chuan, AU - Liu,Xu, AU - Jia,Jizeng, AU - Kong,Xiuying, Y1 - 2023/02/28/ PY - 2022/08/14/received PY - 2023/02/14/revised PY - 2023/02/23/accepted PY - 2023/7/13/medline PY - 2023/3/2/pubmed PY - 2023/3/1/entrez KW - Aegilops KW - B subgenome KW - Sitopsis KW - conserved blocks KW - polyploid wheat SP - 100567 EP - 100567 JF - Plant communications JO - Plant Commun VL - 4 IS - 4 N2 - Wheat (Triticum aestivum, BBAADD) is an allohexaploid species that originated from two polyploidization events. The progenitors of the A and D subgenomes have been identified as Triticum urartu and Aegilops tauschii, respectively. Current research suggests that Aegilops speltoides is the closest but not the direct ancestor of the B subgenome. However, whether Ae. speltoides has contributed genomically to the wheat B subgenome and which chromosome regions are conserved between Ae. speltoides and the B subgenome remain unclear. Here, we assembled a high-quality reference genome for Ae. speltoides, resequenced 53 accessions from seven species (Aegilops bicornis, Aegilops longissima, Aegilops searsii, Aegilops sharonensis, Ae. speltoides, Aegilops mutica [syn. Amblyopyrum muticum], and Triticum dicoccoides) and revealed their genomic contributions to the wheat B subgenome. Our results showed that centromeric regions were particularly conserved between Aegilops and Triticum and revealed 0.17 Gb of conserved blocks between Ae. speltoides and the B subgenome. We classified five groups of conserved and non-conserved genes between Aegilops and Triticum, revealing their biological characteristics, differentiation in gene expression patterns, and collinear relationships between Ae. speltoides and the wheat B subgenome. We also identified gene families that expanded in Ae. speltoides during its evolution and 789 genes specific to Ae. speltoides. These genes can serve as genetic resources for improvement of adaptability to biotic and abiotic stress. The newly constructed reference genome and large-scale resequencing data for Sitopsis species will provide a valuable genomic resource for wheat genetic improvement and genomic studies. SN - 2590-3462 UR - https://www.unboundmedicine.com/medline/citation/36855304/Genome_sequencing_of_Sitopsis_species_provides_insights_into_their_contribution_to_the_B_subgenome_of_bread_wheat_ DB - PRIME DP - Unbound Medicine ER -