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Biomolecular Screening of Pimpinella anisum L. for Antioxidant and Anticholinesterase Activity in Mice Brain.
Molecules. 2023 Feb 27; 28(5)M

Abstract

Hundreds of the plants have been explored and evaluated for antioxidant and anti-amnesic activities, so far. This study was designed to report the biomolecules of Pimpinella anisum L. for the said activities. The aqueous extract of dried P. anisum seeds was fractionated via column chromatography and the fractions so obtained were assessed for the inhibition of acetylcholinesterase (AChE) via in vitro analysis. The fraction which best inhibited AChE was so named as the P. anisum active fraction (P.aAF). The P.aAF was then chemically analyzed via GCMS, which indicated that oxadiazole compounds were present in it. The P.aAF was then administered to albino mice to conduct the in vivo (behavioral and biochemical) studies. The results of the behavioral studies indicated the significant (p < 0.001) increase in inflexion ratio, by the number of hole-pokings through holes and time spent in a dark area by P.aAF treated mice. Biochemical studies demonstrated that the oxadiazole present in P.aAF on one hand presented a noteworthy reduction in MDA and the AChE level and on the other hand promoted the levels of CAT, SOD and GSH in mice brain. The LD50 for P.aAF was calculated as 95 mg/Kg/p.o. The findings thus supported that the antioxidant and anticholinesterase activities of P. anisum are due to its oxadiazole compounds.

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Authors+Show Affiliations

Mushtaq A
Department of Pharmaceutical Sciences, Government College University, Lahore 54000, Pakistan.
Habib F
Gulab Devi Institute of Pharmacy, Gulab Devi Educational Complex, Lahore 54000, Pakistan.
Manea R
Faculty of Medicine, Transilvania University of Brasov, 500036 Brasov, Romania.
Anwar R
Department of Pharmacology, Punjab University College of Pharmacy, University of the Punjab, Lahore 54000, Pakistan.
Gohar UF
Institute of Industrial Biotechnology, Government College University, Lahore 54000, Pakistan.
Zia-Ul-Haq M
Office of Research, Innovation and Commercialization, Lahore College for Women University, Lahore 54000, Pakistan.
Ahmad M
Gulab Devi Institute of Pharmacy, Gulab Devi Educational Complex, Lahore 54000, Pakistan.
Gavris CM
Faculty of Medicine, Transilvania University of Brasov, 500036 Brasov, Romania.
Chicea L
Faculty of Medicine, University "Lucian Blaga" Sibiu, 550169 Sibiu, Romania.

MeSH

MiceAnimalsCholinesterase InhibitorsAntioxidantsPimpinellaAcetylcholinesterasePlant ExtractsBrain

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36903462

Citation

Mushtaq, Aamir, et al. "Biomolecular Screening of Pimpinella Anisum L. for Antioxidant and Anticholinesterase Activity in Mice Brain." Molecules (Basel, Switzerland), vol. 28, no. 5, 2023.
Mushtaq A, Habib F, Manea R, et al. Biomolecular Screening of Pimpinella anisum L. for Antioxidant and Anticholinesterase Activity in Mice Brain. Molecules. 2023;28(5).
Mushtaq, A., Habib, F., Manea, R., Anwar, R., Gohar, U. F., Zia-Ul-Haq, M., Ahmad, M., Gavris, C. M., & Chicea, L. (2023). Biomolecular Screening of Pimpinella anisum L. for Antioxidant and Anticholinesterase Activity in Mice Brain. Molecules (Basel, Switzerland), 28(5). https://doi.org/10.3390/molecules28052217
Mushtaq A, et al. Biomolecular Screening of Pimpinella Anisum L. for Antioxidant and Anticholinesterase Activity in Mice Brain. Molecules. 2023 Feb 27;28(5) PubMed PMID: 36903462.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biomolecular Screening of Pimpinella anisum L. for Antioxidant and Anticholinesterase Activity in Mice Brain. AU - Mushtaq,Aamir, AU - Habib,Fatima, AU - Manea,Rosana, AU - Anwar,Rukhsana, AU - Gohar,Umar Farooq, AU - Zia-Ul-Haq,Muhammad, AU - Ahmad,Mobasher, AU - Gavris,Claudia Mihaela, AU - Chicea,Liana, Y1 - 2023/02/27/ PY - 2023/01/30/received PY - 2023/02/17/revised PY - 2023/02/22/accepted PY - 2023/3/11/entrez PY - 2023/3/12/pubmed PY - 2023/3/15/medline KW - apiol KW - brain KW - cholinergic KW - dementia KW - memory KW - natural products JF - Molecules (Basel, Switzerland) JO - Molecules VL - 28 IS - 5 N2 - Hundreds of the plants have been explored and evaluated for antioxidant and anti-amnesic activities, so far. This study was designed to report the biomolecules of Pimpinella anisum L. for the said activities. The aqueous extract of dried P. anisum seeds was fractionated via column chromatography and the fractions so obtained were assessed for the inhibition of acetylcholinesterase (AChE) via in vitro analysis. The fraction which best inhibited AChE was so named as the P. anisum active fraction (P.aAF). The P.aAF was then chemically analyzed via GCMS, which indicated that oxadiazole compounds were present in it. The P.aAF was then administered to albino mice to conduct the in vivo (behavioral and biochemical) studies. The results of the behavioral studies indicated the significant (p < 0.001) increase in inflexion ratio, by the number of hole-pokings through holes and time spent in a dark area by P.aAF treated mice. Biochemical studies demonstrated that the oxadiazole present in P.aAF on one hand presented a noteworthy reduction in MDA and the AChE level and on the other hand promoted the levels of CAT, SOD and GSH in mice brain. The LD50 for P.aAF was calculated as 95 mg/Kg/p.o. The findings thus supported that the antioxidant and anticholinesterase activities of P. anisum are due to its oxadiazole compounds. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/36903462/Biomolecular_Screening_of_Pimpinella_anisum_L__for_Antioxidant_and_Anticholinesterase_Activity_in_Mice_Brain_ DB - PRIME DP - Unbound Medicine ER -