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Cytoprotection of Probiotic Lactobacillus acidophilus with Artificial Nanoshells of Nature-Derived Eggshell Membrane Hydrolysates and Coffee Melanoidins in Single-Cell Nanoencapsulation.
Polymers (Basel). 2023 Feb 22; 15(5)P

Abstract

One-step fabrication method for thin films and shells is developed with nature-derived eggshell membrane hydrolysates (ESMHs) and coffee melanoidins (CMs) that have been discarded as food waste. The nature-derived polymeric materials, ESMHs and CMs, prove highly biocompatible with living cells, and the one-step method enables cytocompatible construction of cell-in-shell nanobiohybrid structures. Nanometric ESMH-CM shells are formed on individual probiotic Lactobacillus acidophilus, without any noticeable decrease in viability, and the ESMH-CM shells effectively protected L. acidophilus in the simulated gastric fluid (SGF). The cytoprotection power is further enhanced by Fe3+-mediated shell augmentation. For example, after 2 h of incubation in SGF, the viability of native L. acidophilus is 30%, whereas nanoencapsulated L. acidophilus, armed with the Fe3+-fortified ESMH-CM shells, show 79% in viability. The simple, time-efficient, and easy-to-process method developed in this work would contribute to many technological developments, including microbial biotherapeutics, as well as waste upcycling.

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Authors+Show Affiliations

Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon 34141, Republic of Korea.

Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon 34141, Republic of Korea.

Department of Chemistry, University of Ulsan, Ulsan 44776, Republic of Korea.

Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon 34141, Republic of Korea.

Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon 34141, Republic of Korea.

Department of Science Education, Ewha Womans University, Seoul 03760, Republic of Korea.

Center for Cell-Encapsulation Research, Department of Chemistry, KAIST, Daejeon 34141, Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36904345

Citation

Han, Sang Yeong, et al. "Cytoprotection of Probiotic Lactobacillus Acidophilus With Artificial Nanoshells of Nature-Derived Eggshell Membrane Hydrolysates and Coffee Melanoidins in Single-Cell Nanoencapsulation." Polymers, vol. 15, no. 5, 2023.

Han SY, Nguyen DT, Kim BJ, et al. Cytoprotection of Probiotic Lactobacillus acidophilus with Artificial Nanoshells of Nature-Derived Eggshell Membrane Hydrolysates and Coffee Melanoidins in Single-Cell Nanoencapsulation. Polymers (Basel). 2023;15(5).

Han, S. Y., Nguyen, D. T., Kim, B. J., Kim, N., Kang, E. K., Park, J. H., & Choi, I. S. (2023). Cytoprotection of Probiotic Lactobacillus acidophilus with Artificial Nanoshells of Nature-Derived Eggshell Membrane Hydrolysates and Coffee Melanoidins in Single-Cell Nanoencapsulation. Polymers, 15(5). https://doi.org/10.3390/polym15051104

Han SY, et al. Cytoprotection of Probiotic Lactobacillus Acidophilus With Artificial Nanoshells of Nature-Derived Eggshell Membrane Hydrolysates and Coffee Melanoidins in Single-Cell Nanoencapsulation. Polymers (Basel). 2023 Feb 22;15(5) PubMed PMID: 36904345.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Cytoprotection of Probiotic Lactobacillus acidophilus with Artificial Nanoshells of Nature-Derived Eggshell Membrane Hydrolysates and Coffee Melanoidins in Single-Cell Nanoencapsulation. AU - Han,Sang Yeong, AU - Nguyen,Duc Tai, AU - Kim,Beom Jin, AU - Kim,Nayoung, AU - Kang,Eunhye K, AU - Park,Ji Hun, AU - Choi,Insung S, Y1 - 2023/02/22/ PY - 2023/01/21/received PY - 2023/02/14/revised PY - 2023/02/20/accepted PY - 2023/3/11/entrez PY - 2023/3/12/pubmed PY - 2023/3/12/medline KW - Lactobacillus acidophilus KW - coffee melanoidins KW - eggshell membrane hydrolysates KW - probiotics KW - single-cell nanoencapsulation JF - Polymers JO - Polymers (Basel) VL - 15 IS - 5 N2 - One-step fabrication method for thin films and shells is developed with nature-derived eggshell membrane hydrolysates (ESMHs) and coffee melanoidins (CMs) that have been discarded as food waste. The nature-derived polymeric materials, ESMHs and CMs, prove highly biocompatible with living cells, and the one-step method enables cytocompatible construction of cell-in-shell nanobiohybrid structures. Nanometric ESMH-CM shells are formed on individual probiotic Lactobacillus acidophilus, without any noticeable decrease in viability, and the ESMH-CM shells effectively protected L. acidophilus in the simulated gastric fluid (SGF). The cytoprotection power is further enhanced by Fe3+-mediated shell augmentation. For example, after 2 h of incubation in SGF, the viability of native L. acidophilus is 30%, whereas nanoencapsulated L. acidophilus, armed with the Fe3+-fortified ESMH-CM shells, show 79% in viability. The simple, time-efficient, and easy-to-process method developed in this work would contribute to many technological developments, including microbial biotherapeutics, as well as waste upcycling. SN - 2073-4360 UR - https://www.unboundmedicine.com/medline/citation/36904345/Cytoprotection_of_Probiotic_Lactobacillus_acidophilus_with_Artificial_Nanoshells_of_Nature_Derived_Eggshell_Membrane_Hydrolysates_and_Coffee_Melanoidins_in_Single_Cell_Nanoencapsulation_ DB - PRIME DP - Unbound Medicine ER -