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An electrochemical biosensor to identify the phenotype of aggressive breast cancer cells.
Chem Commun (Camb). 2023 Mar 28; 59(26):3890-3893.CC

Abstract

Identifying the phenotype of aggressive breast cancer (BC) cells is vital for the effectiveness of surgical intervention and standard-of-care therapy. HER-2 is overexpressed in aggressive BC and MMP-2 is a crucial indicator of invasiveness and metastasis of BC, so we have proposed an electrochemical biosensor in this work to identify the phenotype of aggressive BC cells via detection of HER-2 together with MMP-2 by designing a dual-trapping peptide and a metal organic framework (MOF)-based probe. Specifically, the designed peptide contains both a HER-2 recognition sequence and MMP-2-specific substrate, while the MOF-based probe (AuNPs@HRP@ZIF-8), prepared by loading horseradish peroxidase (HRP) and gold nanoparticles (AuNPs) on ZIF-8, can also combine with the peptide. Consequently, sensitive and specific detection of both HER-2 and MMP-2 can be achieved in the wide range from 50 fg mL^-1 to 50 ng mL^-1 and 10 fg mL^-1 to 10 ng mL^-1, respectively, and the biosensor can distinguish HER-2⁺ BC cells and evaluate the invasion capability, which might be extended to provide a method for the accurate identification of tumor features in BC subtypes.

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Publisher Full Text (DOI)

Authors+Show Affiliations

State Key Laboratory of Analytical Chemistry for Life Science, School of Life Sciences, Nanjing University, Nanjing, 210023, P. R. China. genxili@nju.edu.cn.

State Key Laboratory of Analytical Chemistry for Life Science, School of Life Sciences, Nanjing University, Nanjing, 210023, P. R. China. genxili@nju.edu.cn.

State Key Laboratory of Analytical Chemistry for Life Science, School of Life Sciences, Nanjing University, Nanjing, 210023, P. R. China. genxili@nju.edu.cn.

State Key Laboratory of Analytical Chemistry for Life Science, School of Life Sciences, Nanjing University, Nanjing, 210023, P. R. China. genxili@nju.edu.cn.

Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai, 200444, P. R. China. jingzhao@t.shu.edu.cn.

State Key Laboratory of Analytical Chemistry for Life Science, School of Life Sciences, Nanjing University, Nanjing, 210023, P. R. China. genxili@nju.edu.cn.

Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai, 200444, P. R. China. jingzhao@t.shu.edu.cn.

State Key Laboratory of Analytical Chemistry for Life Science, School of Life Sciences, Nanjing University, Nanjing, 210023, P. R. China. genxili@nju.edu.cn. Center for Molecular Recognition and Biosensing, School of Life Sciences, Shanghai University, Shanghai, 200444, P. R. China. jingzhao@t.shu.edu.cn.

MeSH

GoldMatrix Metalloproteinase 2Metal NanoparticlesMetal-Organic FrameworksHorseradish PeroxidasePhenotypeBiosensing TechniquesElectrochemical TechniquesLimit of DetectionNeoplasms

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36916831

Citation

Wang, Lin, et al. "An Electrochemical Biosensor to Identify the Phenotype of Aggressive Breast Cancer Cells." Chemical Communications (Cambridge, England), vol. 59, no. 26, 2023, pp. 3890-3893.

Wang L, Xie H, Zhou X, et al. An electrochemical biosensor to identify the phenotype of aggressive breast cancer cells. Chem Commun (Camb). 2023;59(26):3890-3893.

Wang, L., Xie, H., Zhou, X., Lin, Y., Qin, Y., Yang, J., Zhao, J., & Li, G. (2023). An electrochemical biosensor to identify the phenotype of aggressive breast cancer cells. Chemical Communications (Cambridge, England), 59(26), 3890-3893. https://doi.org/10.1039/d3cc00611e

Wang L, et al. An Electrochemical Biosensor to Identify the Phenotype of Aggressive Breast Cancer Cells. Chem Commun (Camb). 2023 Mar 28;59(26):3890-3893. PubMed PMID: 36916831.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - An electrochemical biosensor to identify the phenotype of aggressive breast cancer cells. AU - Wang,Lin, AU - Xie,Haojie, AU - Zhou,Xinyi, AU - Lin,Yuxin, AU - Qin,Yujia, AU - Yang,Jie, AU - Zhao,Jing, AU - Li,Genxi, Y1 - 2023/03/28/ PY - 2023/3/29/medline PY - 2023/3/15/pubmed PY - 2023/3/14/entrez SP - 3890 EP - 3893 JF - Chemical communications (Cambridge, England) JO - Chem Commun (Camb) VL - 59 IS - 26 N2 - Identifying the phenotype of aggressive breast cancer (BC) cells is vital for the effectiveness of surgical intervention and standard-of-care therapy. HER-2 is overexpressed in aggressive BC and MMP-2 is a crucial indicator of invasiveness and metastasis of BC, so we have proposed an electrochemical biosensor in this work to identify the phenotype of aggressive BC cells via detection of HER-2 together with MMP-2 by designing a dual-trapping peptide and a metal organic framework (MOF)-based probe. Specifically, the designed peptide contains both a HER-2 recognition sequence and MMP-2-specific substrate, while the MOF-based probe (AuNPs@HRP@ZIF-8), prepared by loading horseradish peroxidase (HRP) and gold nanoparticles (AuNPs) on ZIF-8, can also combine with the peptide. Consequently, sensitive and specific detection of both HER-2 and MMP-2 can be achieved in the wide range from 50 fg mL-1 to 50 ng mL-1 and 10 fg mL-1 to 10 ng mL-1, respectively, and the biosensor can distinguish HER-2+ BC cells and evaluate the invasion capability, which might be extended to provide a method for the accurate identification of tumor features in BC subtypes. SN - 1364-548X UR - https://www.unboundmedicine.com/medline/citation/36916831/An_electrochemical_biosensor_to_identify_the_phenotype_of_aggressive_breast_cancer_cells_ DB - PRIME DP - Unbound Medicine ER -