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Inhibition of oxidative stress, IL-13, and WNT/β-catenin in ovalbumin-sensitized rats by a novel organogel of Punica granatum seed oil saponifiable fraction.
Biomed Pharmacother. 2022 Oct; 154:113667.BP

Abstract

Bronchial asthma is a chronic inflammatory disease marked by inflammation, oxidative stress, and structural remodeling. Here, we prepared two pomegranate fractions from the seed oil, saponifiable (Sap) and unsaponifiable (UnSap). Two organogels (Orgs) were also formulated with the Sap (Org1) or the UnSap (Org2) fraction and beeswax (BW). All preparations were evaluated in vitro for their antioxidant and anti-inflammatory impacts. The transdermal delivery of the most efficient one was evaluated against ovalbumin (OV)-induced bronchial asthma in rats compared to dexamethasone (DEX). The results showed that the prepared pomegranate fractions and BW had considerable amounts of phenolics (flavonoids and tannins) and triterpenoids. Org1 was shown to be the most effective antioxidant and anti-inflammatory fraction with synergistic activities (combination index, 1), as well as having protective and therapeutic influences on OV-sensitized rats. Org1 inhibited the multiple OV-induced signaling pathways, comprising ROS, WNT/β-catenin, and AKT, with an efficiency superior to DEX. Subsequently, the pro-inflammatory (COX-2, NO, and IL-13), and pro-fibrotic (COL1A1) mediators, oxidative stress, and mucin secretion, were all down-regulated. These outcomes were verified by the histopathological results of lung tissue. Collectively, these outcomes suggest that the transdermal delivery of Org1 to OV-sensitized rats shows promise in the protection and treatment of the pathological hallmarks of asthma.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Shaban NZ
Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
Mohammed AS
Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
Abu-Serie MM
Department of Medical Biotechnology, Genetic Engineering, and Biotechnology Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg EL-Arab 21934, Alexandria, Egypt.
Maher AM
Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
Habashy NH
Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt. Electronic address: noha.habashi@alexu.edu.eg.

MeSH

MaleAnimalsWnt Signaling PathwayInterleukin-13Oxidative StressOvalbuminRatsGelsPomegranateSeedsPlant OilsAsthma

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36942603

Citation

Shaban, Nadia Z., et al. "Inhibition of Oxidative Stress, IL-13, and WNT/β-catenin in Ovalbumin-sensitized Rats By a Novel Organogel of Punica Granatum Seed Oil Saponifiable Fraction." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 154, 2022, p. 113667.
Shaban NZ, Mohammed AS, Abu-Serie MM, et al. Inhibition of oxidative stress, IL-13, and WNT/β-catenin in ovalbumin-sensitized rats by a novel organogel of Punica granatum seed oil saponifiable fraction. Biomed Pharmacother. 2022;154:113667.
Shaban, N. Z., Mohammed, A. S., Abu-Serie, M. M., Maher, A. M., & Habashy, N. H. (2022). Inhibition of oxidative stress, IL-13, and WNT/β-catenin in ovalbumin-sensitized rats by a novel organogel of Punica granatum seed oil saponifiable fraction. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 154, 113667. https://doi.org/10.1016/j.biopha.2022.113667
Shaban NZ, et al. Inhibition of Oxidative Stress, IL-13, and WNT/β-catenin in Ovalbumin-sensitized Rats By a Novel Organogel of Punica Granatum Seed Oil Saponifiable Fraction. Biomed Pharmacother. 2022;154:113667. PubMed PMID: 36942603.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of oxidative stress, IL-13, and WNT/β-catenin in ovalbumin-sensitized rats by a novel organogel of Punica granatum seed oil saponifiable fraction. AU - Shaban,Nadia Z, AU - Mohammed,Alaa S, AU - Abu-Serie,Marwa M, AU - Maher,Adham M, AU - Habashy,Noha H, Y1 - 2022/09/07/ PY - 2022/7/27/received PY - 2022/8/31/revised PY - 2022/9/5/accepted PY - 2023/3/21/entrez PY - 2023/3/22/pubmed PY - 2023/3/23/medline KW - Bronchial asthma KW - IL-13 KW - Oxidative stress KW - Pomegranate organogel KW - WNT/β-catenin SP - 113667 EP - 113667 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed Pharmacother VL - 154 N2 - Bronchial asthma is a chronic inflammatory disease marked by inflammation, oxidative stress, and structural remodeling. Here, we prepared two pomegranate fractions from the seed oil, saponifiable (Sap) and unsaponifiable (UnSap). Two organogels (Orgs) were also formulated with the Sap (Org1) or the UnSap (Org2) fraction and beeswax (BW). All preparations were evaluated in vitro for their antioxidant and anti-inflammatory impacts. The transdermal delivery of the most efficient one was evaluated against ovalbumin (OV)-induced bronchial asthma in rats compared to dexamethasone (DEX). The results showed that the prepared pomegranate fractions and BW had considerable amounts of phenolics (flavonoids and tannins) and triterpenoids. Org1 was shown to be the most effective antioxidant and anti-inflammatory fraction with synergistic activities (combination index, 1), as well as having protective and therapeutic influences on OV-sensitized rats. Org1 inhibited the multiple OV-induced signaling pathways, comprising ROS, WNT/β-catenin, and AKT, with an efficiency superior to DEX. Subsequently, the pro-inflammatory (COX-2, NO, and IL-13), and pro-fibrotic (COL1A1) mediators, oxidative stress, and mucin secretion, were all down-regulated. These outcomes were verified by the histopathological results of lung tissue. Collectively, these outcomes suggest that the transdermal delivery of Org1 to OV-sensitized rats shows promise in the protection and treatment of the pathological hallmarks of asthma. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/36942603/Inhibition_of_oxidative_stress_IL_13_and_WNT/β_catenin_in_ovalbumin_sensitized_rats_by_a_novel_organogel_of_Punica_granatum_seed_oil_saponifiable_fraction_ DB - PRIME DP - Unbound Medicine ER -