The potential of Bacillus and Enterococcus probiotic strains to combat helicobacter pylori attachment to the biotic and abiotic surfaces.
Int Microbiol. 2023 Mar 21 [Online ahead of print]IM

Abstract

The prevention of biofilm formation plays a pivotal role in managing Helicobacter pylori inside the body and the environment. This study showed in vitro potentials of two recently isolated probiotic strains, Bacillus sp. 1630F and Enterococcus sp. 7C37, to form biofilm and combat H. pylori attachment to the abiotic and biotic surfaces. Lactobacillus casei and Bifidobacterium bifidum were used as the reference probiotics. The biofilm rates were the highest in the solid-liquid interface for Lactobacillus and Bifidobacterium and the air-liquid interface for Bacillus and Enterococcus. The highest tolerances to the environmental conditions were observed during the biofilm formations of Enterococcus and Bifidobacterium (pH), Enterococcus and Bacillus (bile), and Bifidobacterium and Lactobacillus (NaCl) on the polystyrene and glass substratum, respectively. Biofilms occurred more quickly by Bacillus and Enterococcus strains than reference strains on the polystyrene and glass substratum, respectively. Enterococcus (competition) and Bacillus (exclusion) achieved the most inhibition of H. pylori biofilm formations on the polystyrene and AGS cells, respectively. Expression of luxS was promoted by Bacillus (exclusion, 3.2 fold) and Enterococcus (competition, 2.0 fold). Expression of ropD was decreased when H. pylori biofilm was excluded by Bacillus (0.4 fold) and Enterococcus (0.2 fold) cells. This study demonstrated the ability of Bacillus and Enterococcus probiotic bacteria to form biofilm and combat H. pylori biofilm formation.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Farrokhi Y

Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.

Neshati Z

Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran. Novel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran.

Saniee P

Department of Microbiology and Microbial Biotechnology, Faculty of Life Science and Biotechnology, Shahid Beheshti University G. C, Tehran, Iran.

Makhdoumi A

Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran. a.makhdomi@um.ac.ir.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36943595

Citation

Farrokhi, Yeganeh, et al. "The Potential of Bacillus and Enterococcus Probiotic Strains to Combat Helicobacter Pylori Attachment to the Biotic and Abiotic Surfaces." International Microbiology : the Official Journal of the Spanish Society for Microbiology, 2023.

Farrokhi Y, Neshati Z, Saniee P, et al. The potential of Bacillus and Enterococcus probiotic strains to combat helicobacter pylori attachment to the biotic and abiotic surfaces. Int Microbiol. 2023.

Farrokhi, Y., Neshati, Z., Saniee, P., & Makhdoumi, A. (2023). The potential of Bacillus and Enterococcus probiotic strains to combat helicobacter pylori attachment to the biotic and abiotic surfaces. International Microbiology : the Official Journal of the Spanish Society for Microbiology. https://doi.org/10.1007/s10123-023-00347-z

Farrokhi Y, et al. The Potential of Bacillus and Enterococcus Probiotic Strains to Combat Helicobacter Pylori Attachment to the Biotic and Abiotic Surfaces. Int Microbiol. 2023 Mar 21; PubMed PMID: 36943595.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - The potential of Bacillus and Enterococcus probiotic strains to combat helicobacter pylori attachment to the biotic and abiotic surfaces. AU - Farrokhi,Yeganeh, AU - Neshati,Zeinab, AU - Saniee,Parastoo, AU - Makhdoumi,Ali, Y1 - 2023/03/21/ PY - 2022/11/21/received PY - 2023/03/16/accepted PY - 2022/12/04/revised PY - 2023/3/21/entrez PY - 2023/3/22/pubmed PY - 2023/3/22/medline KW - Bacillus KW - Biofilm KW - Enterococcus KW - Helicobacter pylori KW - Probiotic JF - International microbiology : the official journal of the Spanish Society for Microbiology JO - Int Microbiol N2 - The prevention of biofilm formation plays a pivotal role in managing Helicobacter pylori inside the body and the environment. This study showed in vitro potentials of two recently isolated probiotic strains, Bacillus sp. 1630F and Enterococcus sp. 7C37, to form biofilm and combat H. pylori attachment to the abiotic and biotic surfaces. Lactobacillus casei and Bifidobacterium bifidum were used as the reference probiotics. The biofilm rates were the highest in the solid-liquid interface for Lactobacillus and Bifidobacterium and the air-liquid interface for Bacillus and Enterococcus. The highest tolerances to the environmental conditions were observed during the biofilm formations of Enterococcus and Bifidobacterium (pH), Enterococcus and Bacillus (bile), and Bifidobacterium and Lactobacillus (NaCl) on the polystyrene and glass substratum, respectively. Biofilms occurred more quickly by Bacillus and Enterococcus strains than reference strains on the polystyrene and glass substratum, respectively. Enterococcus (competition) and Bacillus (exclusion) achieved the most inhibition of H. pylori biofilm formations on the polystyrene and AGS cells, respectively. Expression of luxS was promoted by Bacillus (exclusion, 3.2 fold) and Enterococcus (competition, 2.0 fold). Expression of ropD was decreased when H. pylori biofilm was excluded by Bacillus (0.4 fold) and Enterococcus (0.2 fold) cells. This study demonstrated the ability of Bacillus and Enterococcus probiotic bacteria to form biofilm and combat H. pylori biofilm formation. SN - 1618-1905 UR - https://www.unboundmedicine.com/medline/citation/36943595/The_potential_of_Bacillus_and_Enterococcus_probiotic_strains_to_combat_helicobacter_pylori_attachment_to_the_biotic_and_abiotic_surfaces_ DB - PRIME DP - Unbound Medicine ER -

Tags

The potential of Bacillus and Enterococcus probiotic strains to combat helicobacter pylori attachment to the biotic and abiotic surfaces.Int Microbiol. 2023 Mar 21 [Online ahead of print]IM