Tags

Type your tag names separated by a space and hit enter

Influence of Defatting and Pasteurization on Nutrients and Oxidative Stress Markers in Human Milk.
J Hum Lact. 2023 May; 39(2):278-287.JH

Abstract

BACKGROUND

It is well known that the best nutritional option for infants is human milk, and that when breastfeeding is not possible, human milk banks are a possible alternative. However, in the case of infants with fat transport disorder like chylothorax, defatting of human milk is mandatory.

RESEARCH AIM

The aim of the study was to reduce milk fat content without reducing other nutrients, increasing oxidative stress, or introducing harmful microorganisms.

METHODS

In this prospective, cross-sectional, observational study, we examined the influence of defatting and pasteurization of 50 donor samples on fat, macro- and micronutrients, as well as on oxidative stress markers.

RESULTS

Low-temperature centrifugation proved to be very efficient in defatting, reducing the concentration of triglycerides by 85% and cholesterol by 50%. The macronutrients (proteins, albumin, and Immunoglobulin A) did not undergo significant changes due to defatting and pasteurization procedures, while iron decreased by 36%. However, as the majority of iron is retained, this result does not remarkably change the milk composition. Furthermore, oxidative stress markers and antioxidant levels were unchanged, and the milk result was microbiologically safe.

CONCLUSIONS

Cold milk centrifugation proved to be an effective technique that allows the reduction of human milk lipids. The determination of triglycerides and cholesterol can be used as an indicator of skimming. This procedure is not accompanied by substantial modifications of other components present in the milk.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

D'Alessandro A
Clinical Laboratory Unit, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
Pastore A
Research Unit of Diagnostic and Management Innovations, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
Amadio P
Neonatal Intensive Care Unit and Human Milk Bank, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
D'Agostini M
Clinical Laboratory Unit, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
Terreri S
Diagnostic Immunology Research Unit, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
Carsetti R
Diagnostic Immunology Research Unit, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
Argentieri M
Microbiology Laboratory, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
Bernaschi P
Microbiology Laboratory, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
Onetti Muda A
Research Unit of Diagnostic and Management Innovations, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
Porzio O
Clinical Laboratory Unit, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy. Department of Experimental Medicine, University of Rome "Tor Vergata", Rome, Italy.
Dotta A
Neonatal Intensive Care Unit and Human Milk Bank, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.
Salvatori G
Neonatal Intensive Care Unit and Human Milk Bank, Bambino Gesù Children's Hospital, IRCCS, Rome, Italy.

MeSH

InfantFemaleHumansMilk, HumanPasteurizationCross-Sectional StudiesProspective StudiesBreast FeedingNutrientsMilk BanksTriglyceridesOxidative Stress

Pub Type(s)

Observational Study
Journal Article

Language

eng

PubMed ID

36945737

Citation

D'Alessandro, Annamaria, et al. "Influence of Defatting and Pasteurization On Nutrients and Oxidative Stress Markers in Human Milk." Journal of Human Lactation : Official Journal of International Lactation Consultant Association, vol. 39, no. 2, 2023, pp. 278-287.
D'Alessandro A, Pastore A, Amadio P, et al. Influence of Defatting and Pasteurization on Nutrients and Oxidative Stress Markers in Human Milk. J Hum Lact. 2023;39(2):278-287.
D'Alessandro, A., Pastore, A., Amadio, P., D'Agostini, M., Terreri, S., Carsetti, R., Argentieri, M., Bernaschi, P., Onetti Muda, A., Porzio, O., Dotta, A., & Salvatori, G. (2023). Influence of Defatting and Pasteurization on Nutrients and Oxidative Stress Markers in Human Milk. Journal of Human Lactation : Official Journal of International Lactation Consultant Association, 39(2), 278-287. https://doi.org/10.1177/08903344231156894
D'Alessandro A, et al. Influence of Defatting and Pasteurization On Nutrients and Oxidative Stress Markers in Human Milk. J Hum Lact. 2023;39(2):278-287. PubMed PMID: 36945737.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of Defatting and Pasteurization on Nutrients and Oxidative Stress Markers in Human Milk. AU - D'Alessandro,Annamaria, AU - Pastore,Anna, AU - Amadio,Patrizia, AU - D'Agostini,Matteo, AU - Terreri,Sara, AU - Carsetti,Rita, AU - Argentieri,Marta, AU - Bernaschi,Paola, AU - Onetti Muda,Andrea, AU - Porzio,Ottavia, AU - Dotta,Andrea, AU - Salvatori,Guglielmo, Y1 - 2023/03/21/ PY - 2023/4/20/medline PY - 2023/3/23/pubmed PY - 2023/3/22/entrez KW - IgA KW - antioxidant KW - breastfeeding KW - defatting procedure KW - human milk—biochemistry KW - lactation KW - macronutrients KW - micronutrients KW - oxidative stress markers KW - prospective cross-sectional observational study SP - 278 EP - 287 JF - Journal of human lactation : official journal of International Lactation Consultant Association JO - J Hum Lact VL - 39 IS - 2 N2 - BACKGROUND: It is well known that the best nutritional option for infants is human milk, and that when breastfeeding is not possible, human milk banks are a possible alternative. However, in the case of infants with fat transport disorder like chylothorax, defatting of human milk is mandatory. RESEARCH AIM: The aim of the study was to reduce milk fat content without reducing other nutrients, increasing oxidative stress, or introducing harmful microorganisms. METHODS: In this prospective, cross-sectional, observational study, we examined the influence of defatting and pasteurization of 50 donor samples on fat, macro- and micronutrients, as well as on oxidative stress markers. RESULTS: Low-temperature centrifugation proved to be very efficient in defatting, reducing the concentration of triglycerides by 85% and cholesterol by 50%. The macronutrients (proteins, albumin, and Immunoglobulin A) did not undergo significant changes due to defatting and pasteurization procedures, while iron decreased by 36%. However, as the majority of iron is retained, this result does not remarkably change the milk composition. Furthermore, oxidative stress markers and antioxidant levels were unchanged, and the milk result was microbiologically safe. CONCLUSIONS: Cold milk centrifugation proved to be an effective technique that allows the reduction of human milk lipids. The determination of triglycerides and cholesterol can be used as an indicator of skimming. This procedure is not accompanied by substantial modifications of other components present in the milk. SN - 1552-5732 UR - https://www.unboundmedicine.com/medline/citation/36945737/Influence_of_Defatting_and_Pasteurization_on_Nutrients_and_Oxidative_Stress_Markers_in_Human_Milk_ DB - PRIME DP - Unbound Medicine ER -