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Development and evaluation of an electrochemical biosensor for creatinine quantification in a drop of whole human blood.
Clin Chim Acta. 2023 Mar 21; 543:117300.CC

Abstract

An electrochemical biosensor for creatinine determination in a drop of whole human blood was developed and applied to the determination of creatinine in real clinical samples. It is based on the modification of a dual carbon working electrode with a combination of three enzymes: creatinine amidohydrolase (CNN), creatine amidinohydrolase (CRN) and sarcosine oxidase (SOX). Electrochemical transduction is performed using horseradish peroxidase (HRP) and potassium hexacyanoferrate(II) as mediator. A drop of human blood is enough to carry out the measurements by differential chronoamperometry where one carbon electrode detects creatine and the other both creatine and creatinine. The integrated differential signal obtained in the biosensor is linear with the concentration of creatinine in blood in the range 0.5-15 mg/dL and the enzyme-modified electrodes are stable for at least 3 months at 4 °C. The biosensor was lined to a reference method based on Isotope Dilution Mass Spectrometry (IDMS) with 50 real human blood samples and the results compared with those obtained by alternative routine techniques based on Jaffé method and an enzymatic method (Cobas 8000 Roche®, Crep2 Roche®). There were no significant differences between the creatinine concentrations found by the routine techniques and the developed biosensor.

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Authors+Show Affiliations

Álvarez Menéndez G
Healthsens S.L, Vivarium Ciencias de la Salud, calle Colegio Santo Domingo de Guzmán s/n, 33011 Oviedo, Spain.
Amor-Gutiérrez O
Healthsens S.L, Vivarium Ciencias de la Salud, calle Colegio Santo Domingo de Guzmán s/n, 33011 Oviedo, Spain.
Costa García A
Healthsens S.L, Vivarium Ciencias de la Salud, calle Colegio Santo Domingo de Guzmán s/n, 33011 Oviedo, Spain; Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain.
Funes-Menéndez M
Healthsens S.L, Vivarium Ciencias de la Salud, calle Colegio Santo Domingo de Guzmán s/n, 33011 Oviedo, Spain.
Prado C
Healthsens S.L, Vivarium Ciencias de la Salud, calle Colegio Santo Domingo de Guzmán s/n, 33011 Oviedo, Spain.
Miguel D
Servicio de Bioquímica Clínica, HUCA, Oviedo, Spain.
Rodríguez-González P
Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain.
González-Gago A
Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain.
García Alonso JI
Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Julián Clavería 8, 33006 Oviedo, Spain. Electronic address: jiga@uniovi.es.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36948239

Citation

Álvarez Menéndez, Gabriel, et al. "Development and Evaluation of an Electrochemical Biosensor for Creatinine Quantification in a Drop of Whole Human Blood." Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 543, 2023, p. 117300.
Álvarez Menéndez G, Amor-Gutiérrez O, Costa García A, et al. Development and evaluation of an electrochemical biosensor for creatinine quantification in a drop of whole human blood. Clin Chim Acta. 2023;543:117300.
Álvarez Menéndez, G., Amor-Gutiérrez, O., Costa García, A., Funes-Menéndez, M., Prado, C., Miguel, D., Rodríguez-González, P., González-Gago, A., & García Alonso, J. I. (2023). Development and evaluation of an electrochemical biosensor for creatinine quantification in a drop of whole human blood. Clinica Chimica Acta; International Journal of Clinical Chemistry, 543, 117300. https://doi.org/10.1016/j.cca.2023.117300
Álvarez Menéndez G, et al. Development and Evaluation of an Electrochemical Biosensor for Creatinine Quantification in a Drop of Whole Human Blood. Clin Chim Acta. 2023 Mar 21;543:117300. PubMed PMID: 36948239.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and evaluation of an electrochemical biosensor for creatinine quantification in a drop of whole human blood. AU - Álvarez Menéndez,Gabriel, AU - Amor-Gutiérrez,Olaya, AU - Costa García,Agustín, AU - Funes-Menéndez,María, AU - Prado,Catuxa, AU - Miguel,Diego, AU - Rodríguez-González,Pablo, AU - González-Gago,Adriana, AU - García Alonso,J Ignacio, Y1 - 2023/03/21/ PY - 2022/11/30/received PY - 2023/03/15/revised PY - 2023/03/15/accepted PY - 2023/3/23/pubmed PY - 2023/3/23/medline PY - 2023/3/22/entrez KW - Biosensor KW - Creatinine KW - Human blood SP - 117300 EP - 117300 JF - Clinica chimica acta; international journal of clinical chemistry JO - Clin Chim Acta VL - 543 N2 - An electrochemical biosensor for creatinine determination in a drop of whole human blood was developed and applied to the determination of creatinine in real clinical samples. It is based on the modification of a dual carbon working electrode with a combination of three enzymes: creatinine amidohydrolase (CNN), creatine amidinohydrolase (CRN) and sarcosine oxidase (SOX). Electrochemical transduction is performed using horseradish peroxidase (HRP) and potassium hexacyanoferrate(II) as mediator. A drop of human blood is enough to carry out the measurements by differential chronoamperometry where one carbon electrode detects creatine and the other both creatine and creatinine. The integrated differential signal obtained in the biosensor is linear with the concentration of creatinine in blood in the range 0.5-15 mg/dL and the enzyme-modified electrodes are stable for at least 3 months at 4 °C. The biosensor was lined to a reference method based on Isotope Dilution Mass Spectrometry (IDMS) with 50 real human blood samples and the results compared with those obtained by alternative routine techniques based on Jaffé method and an enzymatic method (Cobas 8000 Roche®, Crep2 Roche®). There were no significant differences between the creatinine concentrations found by the routine techniques and the developed biosensor. SN - 1873-3492 UR - https://www.unboundmedicine.com/medline/citation/36948239/Development_and_evaluation_of_an_electrochemical_biosensor_for_creatinine_quantification_in_a_drop_of_whole_human_blood_ DB - PRIME DP - Unbound Medicine ER -