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Dumbbell Aptamer Sensor Based on Dual Biomarkers for Early Detection of Alzheimer's Disease.
ACS Appl Mater Interfaces. 2023 Apr 05; 15(13):16394-16407.AA

Abstract

Finding a timely, sensitive, and noninvasive detection method has become an urgent need for asymptomatic early diagnosis of Alzheimer's disease (AD). MicroRNA-193b (miR-193b) and Aβ42 oligomers (AβO42) in neurogenic exosomes were confirmed to reflect pathological changes in the AD early stage. The combination of two biomarkers is promising for the earlier detection of AD. In this study, a detection system based on the principle of the entropy-driven strand displacement reaction (ESDR) was developed, including a dumbbell detection probe (H), an indicator probe (R), and graphene oxide (GO). In the detection system, the two hairpins of H were opened by the interaction of miR-193b (T1) and AβO42 (T2) with the aptamer. Then R hybridized with H and began to displace T, initiating the next round of ESDR to achieve sensitive detection of T. GO specifically adsorbed free R and quenched the fluorescence, further reducing the intensity of the background signal. Both of these points provided the system with a more sensitive analytical performance. The detection limit of miR-193b was 77 pM and the detection limit of AβO42 was 53 pM. This sensor detected the change of "one increase (AβO42) and one decrease (miR-193b)" in the exosome sample. Additionally, results showed that this detection system could distinguish the model of early AD from the non-AD control, which was sufficient for earlier and more sensitive detection of AD. This strategy has strong specificity, high sensitivity, and easy operation, which provides broad prospects for the early diagnosis of AD.

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Publisher Full Text (DOI)

Authors+Show Affiliations

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.

School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.

MeSH

HumansAlzheimer DiseaseAptamers, NucleotideBiomarkersEarly DiagnosisMicroRNAsBiosensing Techniques

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36951764

Citation

Zhou, Jie, et al. "Dumbbell Aptamer Sensor Based On Dual Biomarkers for Early Detection of Alzheimer's Disease." ACS Applied Materials & Interfaces, vol. 15, no. 13, 2023, pp. 16394-16407.

Zhou J, Sun Y, Zhang J, et al. Dumbbell Aptamer Sensor Based on Dual Biomarkers for Early Detection of Alzheimer's Disease. ACS Appl Mater Interfaces. 2023;15(13):16394-16407.

Zhou, J., Sun, Y., Zhang, J., Luo, F., Ma, H., Guan, M., Feng, J., & Dong, X. (2023). Dumbbell Aptamer Sensor Based on Dual Biomarkers for Early Detection of Alzheimer's Disease. ACS Applied Materials & Interfaces, 15(13), 16394-16407. https://doi.org/10.1021/acsami.2c21379

Zhou J, et al. Dumbbell Aptamer Sensor Based On Dual Biomarkers for Early Detection of Alzheimer's Disease. ACS Appl Mater Interfaces. 2023 Apr 5;15(13):16394-16407. PubMed PMID: 36951764.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Dumbbell Aptamer Sensor Based on Dual Biomarkers for Early Detection of Alzheimer's Disease. AU - Zhou,Jie, AU - Sun,Yiwen, AU - Zhang,Jin, AU - Luo,Fusui, AU - Ma,Huili, AU - Guan,Min, AU - Feng,Junfen, AU - Dong,Xiaomeng, Y1 - 2023/03/23/ PY - 2023/4/7/medline PY - 2023/3/24/pubmed PY - 2023/3/23/entrez KW - Alzheimer’s disease KW - dumbbell aptamer KW - early detection KW - exosomes KW - sensitive detection SP - 16394 EP - 16407 JF - ACS applied materials & interfaces JO - ACS Appl Mater Interfaces VL - 15 IS - 13 N2 - Finding a timely, sensitive, and noninvasive detection method has become an urgent need for asymptomatic early diagnosis of Alzheimer's disease (AD). MicroRNA-193b (miR-193b) and Aβ42 oligomers (AβO42) in neurogenic exosomes were confirmed to reflect pathological changes in the AD early stage. The combination of two biomarkers is promising for the earlier detection of AD. In this study, a detection system based on the principle of the entropy-driven strand displacement reaction (ESDR) was developed, including a dumbbell detection probe (H), an indicator probe (R), and graphene oxide (GO). In the detection system, the two hairpins of H were opened by the interaction of miR-193b (T1) and AβO42 (T2) with the aptamer. Then R hybridized with H and began to displace T, initiating the next round of ESDR to achieve sensitive detection of T. GO specifically adsorbed free R and quenched the fluorescence, further reducing the intensity of the background signal. Both of these points provided the system with a more sensitive analytical performance. The detection limit of miR-193b was 77 pM and the detection limit of AβO42 was 53 pM. This sensor detected the change of "one increase (AβO42) and one decrease (miR-193b)" in the exosome sample. Additionally, results showed that this detection system could distinguish the model of early AD from the non-AD control, which was sufficient for earlier and more sensitive detection of AD. This strategy has strong specificity, high sensitivity, and easy operation, which provides broad prospects for the early diagnosis of AD. SN - 1944-8252 UR - https://www.unboundmedicine.com/medline/citation/36951764/Dumbbell_Aptamer_Sensor_Based_on_Dual_Biomarkers_for_Early_Detection_of_Alzheimer's_Disease_ DB - PRIME DP - Unbound Medicine ER -