TY - JOUR T1 - Impact of changes in human airway epithelial cellular composition and differentiation on SARS-CoV-2 infection biology. AU - Thaler,Melissa, AU - Wang,Ying, AU - van der Does,Anne M, AU - Faiz,Alen, AU - Ninaber,Dennis K, AU - Ogando,Natacha S, AU - Beckert,Hendrik, AU - Taube,Christian, AU - Salgado-Benvindo,Clarisse, AU - Snijder,Eric J, AU - Bredenbeek,Peter J, AU - Hiemstra,Pieter S, AU - van Hemert,Martijn J, Y1 - 2023/03/25/ PY - 2022/10/13/received PY - 2023/03/03/accepted PY - 2023/3/26/entrez PY - 2023/3/27/pubmed PY - 2023/3/27/medline JF - Journal of innate immunity JO - J Innate Immun N2 - The consequences of infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can range from asymptomatic to fatal disease. Variations in epithelial susceptibility to SARS-CoV-2 infection depend on the anatomical location from the proximal to distal respiratory tract. However, the cellular biology underlying these variations is not completely understood. Thus, air-liquid interface (ALI) cultures of well-differentiated primary human tracheal and bronchial epithelial cells were employed to study the impact of epithelial cellular composition and differentiation on SARS-CoV-2 infection by transcriptional (RNA sequencing) and immunofluorescent analyses. Changes of cellular composition were investigated by varying time of differentiation or by using specific compounds. We found that SARS-CoV-2 primarily infected ciliated cells but also goblet cells and transient secretory cells. Viral replication was impacted by differences in cellular composition, which depended on culturing time and anatomical origin. A higher percentage of ciliated cells correlated with a higher viral load. However, DAPT-treatment, which increased number of ciliated cells and reduced goblet cells, decreased viral load, indicating the contribution of goblet cells to infection. Cell-entry factors, especially cathepsin L and transmembrane protease serine 2, were also affected by differentiation time. In conclusion, our study demonstrates that viral replication is affected by changes in cellular composition, especially in cells related to the mucociliary system. This could explain in part the variable susceptibility to SARS-CoV-2 infection between individuals and between anatomical locations in the respiratory tract. SN - 1662-8128 UR - https://www.unboundmedicine.com/medline/citation/36966527/Impact_of_changes_in_human_airway_epithelial_cellular_composition_and_differentiation_on_SARS_CoV_2_infection_biology_ DB - PRIME DP - Unbound Medicine ER -