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Capabilities of Double-Resonance LPG and SPR Methods for Hypersensitive Detection of SARS-CoV-2 Structural Proteins: A Comparative Study.
Biosensors (Basel). 2023 Feb 24; 13(3)B

Abstract

The danger of the emergence of new viral diseases and their rapid spread demands apparatuses for continuous rapid monitoring in real time. This requires the creation of new bioanalytical methods that overcome the shortcomings of existing ones and are applicable for point-of-care diagnostics. For this purpose, a variety of biosensors have been developed and tested in proof-of-concept studies, but none of them have been introduced for commercial use so far. Given the importance of the problem, in this study, long-period grating (LPG) and surface plasmon resonance (SPR) biosensors, based on antibody detection, were examined, and their capabilities for SARS-CoV-2 structural proteins detection were established. Supersensitive detections of structural proteins in the order of several femtomoles were achieved by the LPG method, while the SPR method demonstrated a sensitivity of about one hundred femtomoles. The studied biosensors are compatible in sensitivity with ELISA and rapid antigen tests but, in contrast, they are quantitative, which makes them applicable for acute SARS-CoV-2 infection detection, especially during the early stages of viral replication.

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Authors+Show Affiliations

Eftimov T
Photonics Research Center, Université du Québec en Outaouais, Rue 101 St-Jean Bosco, Gatineau, QC J8X 3G5, Canada. Central Laboratory of Applied Physics, Bulgarian Academy of Sciences, 61 Sanct Peterburg Blvd., 4000 Plovdiv, Bulgaria.
Genova-Kalou P
National Center of Infectious and Parasitic Diseases, 44A "Gen. Stoletov" Blvd., 1233 Sofia, Bulgaria.
Dyankov G
Central Laboratory of Applied Physics, Bulgarian Academy of Sciences, 61 Sanct Peterburg Blvd., 4000 Plovdiv, Bulgaria. Institute of Optical Materials and Technologies "Acad. J. Malinowski" (IOMT), Bulgarian Academy of Sciences (BAS), 109 "Acad. G. Bonchev" Str., 1113 Sofia, Bulgaria.
Bock WJ
Photonics Research Center, Université du Québec en Outaouais, Rue 101 St-Jean Bosco, Gatineau, QC J8X 3G5, Canada.
Mankov V
Institute of Optical Materials and Technologies "Acad. J. Malinowski" (IOMT), Bulgarian Academy of Sciences (BAS), 109 "Acad. G. Bonchev" Str., 1113 Sofia, Bulgaria.
Shoar Ghaffari S
Photonics Research Center, Université du Québec en Outaouais, Rue 101 St-Jean Bosco, Gatineau, QC J8X 3G5, Canada.
Veselinov P
Institute of Optical Materials and Technologies "Acad. J. Malinowski" (IOMT), Bulgarian Academy of Sciences (BAS), 109 "Acad. G. Bonchev" Str., 1113 Sofia, Bulgaria.
Arapova A
Photonics Research Center, Université du Québec en Outaouais, Rue 101 St-Jean Bosco, Gatineau, QC J8X 3G5, Canada.
Makouei S
Faculty of Electrical and Computer Engineering, University of Tabriz, Tabriz 5166616471, Iran.

MeSH

HumansSurface Plasmon ResonanceSARS-CoV-2COVID-19Biosensing TechniquesVirus Diseases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36979530

Citation

Eftimov, Tinko, et al. "Capabilities of Double-Resonance LPG and SPR Methods for Hypersensitive Detection of SARS-CoV-2 Structural Proteins: a Comparative Study." Biosensors, vol. 13, no. 3, 2023.
Eftimov T, Genova-Kalou P, Dyankov G, et al. Capabilities of Double-Resonance LPG and SPR Methods for Hypersensitive Detection of SARS-CoV-2 Structural Proteins: A Comparative Study. Biosensors (Basel). 2023;13(3).
Eftimov, T., Genova-Kalou, P., Dyankov, G., Bock, W. J., Mankov, V., Shoar Ghaffari, S., Veselinov, P., Arapova, A., & Makouei, S. (2023). Capabilities of Double-Resonance LPG and SPR Methods for Hypersensitive Detection of SARS-CoV-2 Structural Proteins: A Comparative Study. Biosensors, 13(3). https://doi.org/10.3390/bios13030318
Eftimov T, et al. Capabilities of Double-Resonance LPG and SPR Methods for Hypersensitive Detection of SARS-CoV-2 Structural Proteins: a Comparative Study. Biosensors (Basel). 2023 Feb 24;13(3) PubMed PMID: 36979530.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capabilities of Double-Resonance LPG and SPR Methods for Hypersensitive Detection of SARS-CoV-2 Structural Proteins: A Comparative Study. AU - Eftimov,Tinko, AU - Genova-Kalou,Petia, AU - Dyankov,Georgi, AU - Bock,Wojtek J, AU - Mankov,Vihar, AU - Shoar Ghaffari,Sanaz, AU - Veselinov,Petar, AU - Arapova,Alla, AU - Makouei,Somayeh, Y1 - 2023/02/24/ PY - 2023/01/30/received PY - 2023/02/11/revised PY - 2023/02/16/accepted PY - 2023/3/30/medline PY - 2023/3/29/entrez PY - 2023/3/30/pubmed KW - SARS-CoV-2 KW - biosensors KW - long period grating KW - structural proteins KW - surface plasmon resonance JF - Biosensors JO - Biosensors (Basel) VL - 13 IS - 3 N2 - The danger of the emergence of new viral diseases and their rapid spread demands apparatuses for continuous rapid monitoring in real time. This requires the creation of new bioanalytical methods that overcome the shortcomings of existing ones and are applicable for point-of-care diagnostics. For this purpose, a variety of biosensors have been developed and tested in proof-of-concept studies, but none of them have been introduced for commercial use so far. Given the importance of the problem, in this study, long-period grating (LPG) and surface plasmon resonance (SPR) biosensors, based on antibody detection, were examined, and their capabilities for SARS-CoV-2 structural proteins detection were established. Supersensitive detections of structural proteins in the order of several femtomoles were achieved by the LPG method, while the SPR method demonstrated a sensitivity of about one hundred femtomoles. The studied biosensors are compatible in sensitivity with ELISA and rapid antigen tests but, in contrast, they are quantitative, which makes them applicable for acute SARS-CoV-2 infection detection, especially during the early stages of viral replication. SN - 2079-6374 UR - https://www.unboundmedicine.com/medline/citation/36979530/Capabilities_of_Double_Resonance_LPG_and_SPR_Methods_for_Hypersensitive_Detection_of_SARS_CoV_2_Structural_Proteins:_A_Comparative_Study_ DB - PRIME DP - Unbound Medicine ER -