Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE).
Biomedicines. 2023 Feb 25; 11(3)B

Abstract

The determination of antidepressant drugs and their metabolites in the body, mainly in the blood, allows for the monitoring of drug levels and their metabolism, helps identify drug interactions, and reduces the likelihood of increased side effects. Due to numerous inconveniences associated with collecting blood in patients, therapeutic drug monitoring (TDM) based on saliva sampling could significantly improve patient comfort. Therefore, the aim of this study was to develop a method for the simultaneous determination of selected antidepressants (amitriptyline, mianserin, duloxetine, mirtazapine, sertraline, citalopram, and venlafaxine) and their metabolites (N-desmethylmirtazapine, norsertraline, N-desmethylcitalopram, O-desmethylvenlafaxine) in human saliva using supported liquid extraction (SLE). Chlordiazepoxide was used as an internal standard. UHPLC coupled with DAD detection was used for the determinations. The proposed method was validated by determining its linearity for saliva concentrations in the range 10-1000 ng/mL. For all the analyzed compounds, a linear relationship between the analytical signal and analyte concentration was obtained (R2 > 0.99), with the intra- and inter-day precisions expressed as a coefficient of variation (% CV) below 15% in all tested cases. The study showed the usefulness of the proposed method for the isolation of antidepressant drugs and their metabolites in saliva patients' samples.

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Authors+Show Affiliations

Dziurkowska E

Department of Analytical Chemistry, Medical University of Gdansk, Gen. J. Hallera 107, 80-416 Gdansk, Poland.

Wesolowski M

Department of Analytical Chemistry, Medical University of Gdansk, Gen. J. Hallera 107, 80-416 Gdansk, Poland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36979687

Citation

Dziurkowska, Ewelina, and Marek Wesolowski. "Isolation of Antidepressants and Their Metabolites From Saliva Using Supported Liquid Extraction (SLE)." Biomedicines, vol. 11, no. 3, 2023.

Dziurkowska E, Wesolowski M. Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE). Biomedicines. 2023;11(3).

Dziurkowska, E., & Wesolowski, M. (2023). Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE). Biomedicines, 11(3). https://doi.org/10.3390/biomedicines11030708

Dziurkowska E, Wesolowski M. Isolation of Antidepressants and Their Metabolites From Saliva Using Supported Liquid Extraction (SLE). Biomedicines. 2023 Feb 25;11(3) PubMed PMID: 36979687.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE). AU - Dziurkowska,Ewelina, AU - Wesolowski,Marek, Y1 - 2023/02/25/ PY - 2022/12/22/received PY - 2023/02/13/revised PY - 2023/02/23/accepted PY - 2023/3/30/medline PY - 2023/3/29/entrez PY - 2023/3/30/pubmed KW - UHPLC KW - antidepressants KW - metabolites KW - saliva KW - supported liquid extraction KW - validation JF - Biomedicines JO - Biomedicines VL - 11 IS - 3 N2 - The determination of antidepressant drugs and their metabolites in the body, mainly in the blood, allows for the monitoring of drug levels and their metabolism, helps identify drug interactions, and reduces the likelihood of increased side effects. Due to numerous inconveniences associated with collecting blood in patients, therapeutic drug monitoring (TDM) based on saliva sampling could significantly improve patient comfort. Therefore, the aim of this study was to develop a method for the simultaneous determination of selected antidepressants (amitriptyline, mianserin, duloxetine, mirtazapine, sertraline, citalopram, and venlafaxine) and their metabolites (N-desmethylmirtazapine, norsertraline, N-desmethylcitalopram, O-desmethylvenlafaxine) in human saliva using supported liquid extraction (SLE). Chlordiazepoxide was used as an internal standard. UHPLC coupled with DAD detection was used for the determinations. The proposed method was validated by determining its linearity for saliva concentrations in the range 10-1000 ng/mL. For all the analyzed compounds, a linear relationship between the analytical signal and analyte concentration was obtained (R2 > 0.99), with the intra- and inter-day precisions expressed as a coefficient of variation (% CV) below 15% in all tested cases. The study showed the usefulness of the proposed method for the isolation of antidepressant drugs and their metabolites in saliva patients' samples. SN - 2227-9059 UR - https://www.unboundmedicine.com/medline/citation/36979687/Isolation_of_Antidepressants_and_Their_Metabolites_from_Saliva_Using_Supported_Liquid_Extraction__SLE__ DB - PRIME DP - Unbound Medicine ER -

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Isolation of Antidepressants and Their Metabolites from Saliva Using Supported Liquid Extraction (SLE).Biomedicines. 2023 Feb 25; 11(3)B