Rapid Detection of the Activity of Lacticaseibacillus Casei Zhang by Flow Cytometry.
Foods. 2023 Mar 12; 12(6)F

Abstract

Food processing, e.g., freeze-drying, exerts strong pressure on bacteria in the food matrix, decreasing their viability/activity and even forcing them to become viable but unculturable (VBNC), which are often underestimated by traditional plate count. The strict standards of bacterial viability in probiotic products require accurate cell viability/activity enumeration. We developed a staining (5(6)-carboxyfluorescein diacetate succinimide ester, propidium iodide)-based flow cytometry rapid method for detecting the viability/activity of Lacticaseibacillus (Lb.) casei Zhang, a widely used probiotic in the dairy industry in China. We optimized the procedural and instrumental parameters for generating results comparable to that of standard plate counts. This method was also applied to freeze-dried Lb. casei Zhang, yielding 7.7 × 1011 CFU/g, which was non-significantly higher than the results obtained by plate count (6.4 × 1011 CFU/g), possibly due to the detection of VBNC cells in the freeze-dried powder. We anticipated that this method can be used for detecting lactic acid bacteria in other probiotic food/beverages.

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Authors+Show Affiliations

Ma X

Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot 010018, China. Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot 010018, China. Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36981135

Citation

Ma, Xuebo, et al. "Rapid Detection of the Activity of Lacticaseibacillus Casei Zhang By Flow Cytometry." Foods (Basel, Switzerland), vol. 12, no. 6, 2023.

Ma X, Wang L, Dai L, et al. Rapid Detection of the Activity of Lacticaseibacillus Casei Zhang by Flow Cytometry. Foods. 2023;12(6).

Ma, X., Wang, L., Dai, L., Kwok, L. Y., & Bao, Q. (2023). Rapid Detection of the Activity of Lacticaseibacillus Casei Zhang by Flow Cytometry. Foods (Basel, Switzerland), 12(6). https://doi.org/10.3390/foods12061208

Ma X, et al. Rapid Detection of the Activity of Lacticaseibacillus Casei Zhang By Flow Cytometry. Foods. 2023 Mar 12;12(6) PubMed PMID: 36981135.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Rapid Detection of the Activity of Lacticaseibacillus Casei Zhang by Flow Cytometry. AU - Ma,Xuebo, AU - Wang,Lina, AU - Dai,Lixia, AU - Kwok,Lai-Yu, AU - Bao,Qiuhua, Y1 - 2023/03/12/ PY - 2023/02/16/received PY - 2023/03/04/revised PY - 2023/03/08/accepted PY - 2023/3/30/medline PY - 2023/3/29/entrez PY - 2023/3/30/pubmed KW - CFDA KW - Lacticaseibacillus casei Zhang KW - activity detection KW - flow cytometry KW - fluorescent dyes KW - plate count KW - propidium iodide JF - Foods (Basel, Switzerland) JO - Foods VL - 12 IS - 6 N2 - Food processing, e.g., freeze-drying, exerts strong pressure on bacteria in the food matrix, decreasing their viability/activity and even forcing them to become viable but unculturable (VBNC), which are often underestimated by traditional plate count. The strict standards of bacterial viability in probiotic products require accurate cell viability/activity enumeration. We developed a staining (5(6)-carboxyfluorescein diacetate succinimide ester, propidium iodide)-based flow cytometry rapid method for detecting the viability/activity of Lacticaseibacillus (Lb.) casei Zhang, a widely used probiotic in the dairy industry in China. We optimized the procedural and instrumental parameters for generating results comparable to that of standard plate counts. This method was also applied to freeze-dried Lb. casei Zhang, yielding 7.7 × 1011 CFU/g, which was non-significantly higher than the results obtained by plate count (6.4 × 1011 CFU/g), possibly due to the detection of VBNC cells in the freeze-dried powder. We anticipated that this method can be used for detecting lactic acid bacteria in other probiotic food/beverages. SN - 2304-8158 UR - https://www.unboundmedicine.com/medline/citation/36981135/Rapid_Detection_of_the_Activity_of_Lacticaseibacillus_Casei_Zhang_by_Flow_Cytometry_ DB - PRIME DP - Unbound Medicine ER -

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Rapid Detection of the Activity of Lacticaseibacillus Casei Zhang by Flow Cytometry.Foods. 2023 Mar 12; 12(6)F