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Campomanesia lineatifolia Ruiz & Pavón (Myrtaceae): Isolation of major and minor compounds of phenolic-rich extract by high-speed countercurrent chromatography and anti-inflammatory evaluation.
J Ethnopharmacol. 2023 Jun 28; 310:116417.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Campomanesia lineatifolia Ruiz & Pavón (Myrtaceae), an edible species found in Brazilian Forest, possesses leaves that are traditionally used for the treatment of gastrointestinal disorders in Brazil. Extracts of C. lineatifolia are rich in phenolics and exhibit antioxidant, and gastric antiulcer properties. Furthermore, Campomanesia spp. have been described to possess anti-inflammatory properties, but studies related to chemical constituents of C. lineatifolia are scarce in the literature.

AIM OF THE STUDY

This work aims to identify the chemical composition of the phenolic-rich ethanol extract (PEE) from C. lineatifolia leaves and evaluate the anti-inflammatory activity that could be related to its ethnopharmacological use.

MATERIALS AND METHODS

The high-speed countercurrent chromatography (HSCCC), using an isocratic and a step gradient elution method, and NMR, HPLC-ESI-QTOF-MS/MS were used to isolate and identify the chemicals of PEE, respectively. Lipopolysaccharide-(LPS)-stimulated THP-1 cells were used to evaluate the anti-inflammatory activities from PEE and the two majority flavonoids isolated by measure TNF-α and NF-κB inhibition assays.

RESULTS

Fourteen compounds were isolated from the PEE, further identified by NMR and HPLC-ESI-QTOF-MS/MS, twelve of them are new compounds, and two others are already known for the species. The PEE, quercitrin and myricitrin promoted a concentration-dependent inhibition of TNF-α, and PEE promoted an inhibition of NF-κB pathway.

CONCLUSIONS

PEE from C. lineatifolia leaves demonstrated significant anti-inflammatory activity that may be related to the traditional use to treat gastrointestinal disorders.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Neves NCV
GnosiaH, Pharmacognosy and Homeopathy Laboratory, School of Pharmacy, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Campus Pampulha, 31.270-901, Belo Horizonte, MG, Brazil; School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland; Department of Pharmacy, Centro Universitário Santa Rita, Área Rural, SN, KM 206, Caixa Postal 26, 31.270-901, Conselheiro Lafaiete, MG, Brazil. Electronic address: niveacvn@gmail.com.
de Mello MP
GnosiaH, Pharmacognosy and Homeopathy Laboratory, School of Pharmacy, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Campus Pampulha, 31.270-901, Belo Horizonte, MG, Brazil. Electronic address: morganamello19@gmail.com.
Zaidan I
Signalling in Inflammation Laboratory, Department of Clinical and Toxicological Analysis, School of Pharmacy, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Campus Pampulha, 31.270-901, Belo Horizonte, MG, Brazil. Electronic address: bellazaidanmoreira@gmail.com.
Sousa LP
Signalling in Inflammation Laboratory, Department of Clinical and Toxicological Analysis, School of Pharmacy, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Campus Pampulha, 31.270-901, Belo Horizonte, MG, Brazil. Electronic address: lipsousa72@gmail.com.
Braga AV
Department of Pharmaceutical Products, School of Pharmacy, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Campus Pampulha, 31.270-901, Belo Horizonte, MG, Brazil. Electronic address: alyssonviniciuss@hotmail.com.
Machado RR
Department of Pharmaceutical Products, School of Pharmacy, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Campus Pampulha, 31.270-901, Belo Horizonte, MG, Brazil. Electronic address: rrm_farmacia@hotmail.com.
Kukula-Koch W
Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Lublin, 1 Chodźki Str., 20-093, Lublin, Poland. Electronic address: virginia.kukula@gmail.com.
Boylan F
School of Pharmacy and Pharmaceutical Sciences, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, Ireland. Electronic address: fabio.boylan@tcd.ie.
Caliari MV
Department of General Pathology, Biological Sciences Institute, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Campus Pampulha, 31.270-901, Belo Horizonte, MG, Brazil. Electronic address: caliari@icb.ufmg.br.
Castilho RO
GnosiaH, Pharmacognosy and Homeopathy Laboratory, School of Pharmacy, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Campus Pampulha, 31.270-901, Belo Horizonte, MG, Brazil; Consórcio Acadêmico Brasileiro de Saúde Integrativa, CABSIN, São Paulo, 05449-070, Brazil. Electronic address: roc2006@farmacia.ufmg.br.

MeSH

Plant ExtractsTumor Necrosis Factor-alphaTandem Mass SpectrometryNF-kappa BMyrtaceaeCountercurrent DistributionAnti-Inflammatory AgentsEthanolPlant Leaves

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36990302

Citation

Neves, Nívea Cristina Vieira, et al. "Campomanesia Lineatifolia Ruiz & Pavón (Myrtaceae): Isolation of Major and Minor Compounds of Phenolic-rich Extract By High-speed Countercurrent Chromatography and Anti-inflammatory Evaluation." Journal of Ethnopharmacology, vol. 310, 2023, p. 116417.
Neves NCV, de Mello MP, Zaidan I, et al. Campomanesia lineatifolia Ruiz & Pavón (Myrtaceae): Isolation of major and minor compounds of phenolic-rich extract by high-speed countercurrent chromatography and anti-inflammatory evaluation. J Ethnopharmacol. 2023;310:116417.
Neves, N. C. V., de Mello, M. P., Zaidan, I., Sousa, L. P., Braga, A. V., Machado, R. R., Kukula-Koch, W., Boylan, F., Caliari, M. V., & Castilho, R. O. (2023). Campomanesia lineatifolia Ruiz & Pavón (Myrtaceae): Isolation of major and minor compounds of phenolic-rich extract by high-speed countercurrent chromatography and anti-inflammatory evaluation. Journal of Ethnopharmacology, 310, 116417. https://doi.org/10.1016/j.jep.2023.116417
Neves NCV, et al. Campomanesia Lineatifolia Ruiz & Pavón (Myrtaceae): Isolation of Major and Minor Compounds of Phenolic-rich Extract By High-speed Countercurrent Chromatography and Anti-inflammatory Evaluation. J Ethnopharmacol. 2023 Jun 28;310:116417. PubMed PMID: 36990302.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Campomanesia lineatifolia Ruiz & Pavón (Myrtaceae): Isolation of major and minor compounds of phenolic-rich extract by high-speed countercurrent chromatography and anti-inflammatory evaluation. AU - Neves,Nívea Cristina Vieira, AU - de Mello,Morgana Pinheiro, AU - Zaidan,Isabella, AU - Sousa,Lirlândia Pires, AU - Braga,Alysson Vinícius, AU - Machado,Renes Resende, AU - Kukula-Koch,Wirginia, AU - Boylan,Fabio, AU - Caliari,Marcelo Vidigal, AU - Castilho,Rachel Oliveira, Y1 - 2023/03/28/ PY - 2022/12/28/received PY - 2023/03/17/revised PY - 2023/03/20/accepted PY - 2023/4/11/medline PY - 2023/3/30/pubmed PY - 2023/3/29/entrez KW - Anti-inflammatory KW - Gastric ulcer KW - Guabiroba KW - HSCCC KW - Myricitrin KW - Myrtaceae KW - Peptic ulcer KW - Phenolic-rich KW - Quercitrin SP - 116417 EP - 116417 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 310 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Campomanesia lineatifolia Ruiz & Pavón (Myrtaceae), an edible species found in Brazilian Forest, possesses leaves that are traditionally used for the treatment of gastrointestinal disorders in Brazil. Extracts of C. lineatifolia are rich in phenolics and exhibit antioxidant, and gastric antiulcer properties. Furthermore, Campomanesia spp. have been described to possess anti-inflammatory properties, but studies related to chemical constituents of C. lineatifolia are scarce in the literature. AIM OF THE STUDY: This work aims to identify the chemical composition of the phenolic-rich ethanol extract (PEE) from C. lineatifolia leaves and evaluate the anti-inflammatory activity that could be related to its ethnopharmacological use. MATERIALS AND METHODS: The high-speed countercurrent chromatography (HSCCC), using an isocratic and a step gradient elution method, and NMR, HPLC-ESI-QTOF-MS/MS were used to isolate and identify the chemicals of PEE, respectively. Lipopolysaccharide-(LPS)-stimulated THP-1 cells were used to evaluate the anti-inflammatory activities from PEE and the two majority flavonoids isolated by measure TNF-α and NF-κB inhibition assays. RESULTS: Fourteen compounds were isolated from the PEE, further identified by NMR and HPLC-ESI-QTOF-MS/MS, twelve of them are new compounds, and two others are already known for the species. The PEE, quercitrin and myricitrin promoted a concentration-dependent inhibition of TNF-α, and PEE promoted an inhibition of NF-κB pathway. CONCLUSIONS: PEE from C. lineatifolia leaves demonstrated significant anti-inflammatory activity that may be related to the traditional use to treat gastrointestinal disorders. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/36990302/Campomanesia_lineatifolia_Ruiz_&_Pavón__Myrtaceae_:_Isolation_of_major_and_minor_compounds_of_phenolic_rich_extract_by_high_speed_countercurrent_chromatography_and_anti_inflammatory_evaluation_ DB - PRIME DP - Unbound Medicine ER -