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Glucocorticoid cytosol binding in exercise-induced sparing of muscle atrophy.
J Appl Physiol (1985). 1986 Apr; 60(4):1413-9.JA

Abstract

Female rats were initially divided into a sedentary or an exercise group that was trained by treadmill running to a final work rate of 31 m/min, 100 min/day, for 13-18 wk. During the last 12 days of training each of these groups were further subdivided into groups that received daily subcutaneous injections of cortisol acetate (CA) (100 mg/kg body wt) or the vehicle (1% carboxymethyl cellulose). Exercise prevented approximately 40% of the gastrocnemius muscle weight loss due to CA treatment. Training did not influence glucocorticoid cytosol-receptor binding concentrations, using [3H]triamcinolone acetonide (TA) as the labeled glucocorticoid in any of the skeletal muscle types investigated. TA-receptor binding capacities were depleted by the multiple injections but were higher in the red fiber types of the CA-treated trained than those in the CA-treated sedentary animals. In a second series of experiments in which receptor depletion and repletion rates were studied using a single injection of cortisol, TA binding capacities 2 h after the cortisol injection were higher in slow-twitch red soleus muscles of trained as compared with sedentary rats (36.4 +/- 2.0 vs. 26.8 +/- 2.5 fmol/mg protein). Similar patterns of TA binding were also observed at 2 h between trained and sedentary animals in the fast-twitch red muscle types, whereas no training related differences were observed in white muscle types. Total and free serum cortisol concentrations also returned to base-line values faster in the trained animals following the single injection protocol.(

ABSTRACT

TRUNCATED AT 250 WORDS)

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3700317

Citation

Hickson, R C., et al. "Glucocorticoid Cytosol Binding in Exercise-induced Sparing of Muscle Atrophy." Journal of Applied Physiology (Bethesda, Md. : 1985), vol. 60, no. 4, 1986, pp. 1413-9.
Hickson RC, Kurowski TT, Andrews GH, et al. Glucocorticoid cytosol binding in exercise-induced sparing of muscle atrophy. J Appl Physiol (1985). 1986;60(4):1413-9.
Hickson, R. C., Kurowski, T. T., Andrews, G. H., Capaccio, J. A., & Chatterton, R. T. (1986). Glucocorticoid cytosol binding in exercise-induced sparing of muscle atrophy. Journal of Applied Physiology (Bethesda, Md. : 1985), 60(4), 1413-9.
Hickson RC, et al. Glucocorticoid Cytosol Binding in Exercise-induced Sparing of Muscle Atrophy. J Appl Physiol (1985). 1986;60(4):1413-9. PubMed PMID: 3700317.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Glucocorticoid cytosol binding in exercise-induced sparing of muscle atrophy. AU - Hickson,R C, AU - Kurowski,T T, AU - Andrews,G H, AU - Capaccio,J A, AU - Chatterton,R T,Jr PY - 1986/4/1/pubmed PY - 2001/3/28/medline PY - 1986/4/1/entrez SP - 1413 EP - 9 JF - Journal of applied physiology (Bethesda, Md. : 1985) JO - J Appl Physiol (1985) VL - 60 IS - 4 N2 - Female rats were initially divided into a sedentary or an exercise group that was trained by treadmill running to a final work rate of 31 m/min, 100 min/day, for 13-18 wk. During the last 12 days of training each of these groups were further subdivided into groups that received daily subcutaneous injections of cortisol acetate (CA) (100 mg/kg body wt) or the vehicle (1% carboxymethyl cellulose). Exercise prevented approximately 40% of the gastrocnemius muscle weight loss due to CA treatment. Training did not influence glucocorticoid cytosol-receptor binding concentrations, using [3H]triamcinolone acetonide (TA) as the labeled glucocorticoid in any of the skeletal muscle types investigated. TA-receptor binding capacities were depleted by the multiple injections but were higher in the red fiber types of the CA-treated trained than those in the CA-treated sedentary animals. In a second series of experiments in which receptor depletion and repletion rates were studied using a single injection of cortisol, TA binding capacities 2 h after the cortisol injection were higher in slow-twitch red soleus muscles of trained as compared with sedentary rats (36.4 +/- 2.0 vs. 26.8 +/- 2.5 fmol/mg protein). Similar patterns of TA binding were also observed at 2 h between trained and sedentary animals in the fast-twitch red muscle types, whereas no training related differences were observed in white muscle types. Total and free serum cortisol concentrations also returned to base-line values faster in the trained animals following the single injection protocol.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 8750-7587 UR - https://www.unboundmedicine.com/medline/citation/3700317/Glucocorticoid_cytosol_binding_in_exercise_induced_sparing_of_muscle_atrophy_ L2 - https://journals.physiology.org/doi/10.1152/jappl.1986.60.4.1413?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -