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Versatile FeMoOv nanozyme bipolar electrode electrochemiluminescence biosensing and imaging platform for detection of H2O2 and PSA.
Biosens Bioelectron. 2023 Jul 15; 232:115315.BB

Abstract

In this work, a unique FeMoOv nanozyme-bipolar electrode (NM-BPE) electrochemiluminescence (ECL) biosensing and imaging platform was proposed for the first time to realize sensitive detection of target hydrogen peroxide (H2O2) and prostate specific antigen (PSA). Considering the advantage that the cathode and anode poles of the bipolar electrode (BPE) can be modified respectively, this work was carried out using anode equipped with ECL reagent bipyridine ruthenium (Ru(bpy)3[2+]), and cathode equipped with the Fe-doped molybdenum oxide/Au nanoparticles (FeMoOv/AuNPs) with excellent peroxidase (POD) and catalase (CAT)-like activity. Because FeMoOv/AuNPs show efficient enzyme catalysis effect and can greatly promote the decomposition of H2O2, thus the electron transfer rate in the NM-BPE system would be much accelerated to enhance the ECL signal of Ru(bpy)3[2+]. Based on this principle, this work not only realized sensitive detection of H2O2, but also ingeniously designed an sandwich immunosensor using FeMoOv/AuNPs as recognition probe to mediate the ECL response on the anode, achieving highly sensitive detection of PSA. Furthermore, a unique mobile phone ECL imaging system was developed for assay of PSA at different concentrations, which opened a new portable imaging sensing device for bioassays. This work was the first time to combine nanozymes with bipolar electrodes for ECL analysis and imaging, which not only broadened the applications of nanozymes, but also pioneered the new joint ECL research technique of bipolar electrode and ECL imaging in bioassays, showing great application prospect for multiple detection of proteins, nucleic acids and cancer cells.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Li H
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Cai Q
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Wang J
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China.
Jie G
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China. Electronic address: guifenjie@126.com.

MeSH

HumansMaleProstate-Specific AntigenHydrogen PeroxideLuminescent MeasurementsGoldBiosensing TechniquesMetal NanoparticlesImmunoassayElectrodesElectrochemical Techniques

Pub Type(s)

Journal Article

Language

eng

PubMed ID

37068419

Citation

Li, Hongkun, et al. "Versatile FeMoOv Nanozyme Bipolar Electrode Electrochemiluminescence Biosensing and Imaging Platform for Detection of H2O2 and PSA." Biosensors & Bioelectronics, vol. 232, 2023, p. 115315.
Li H, Cai Q, Wang J, et al. Versatile FeMoOv nanozyme bipolar electrode electrochemiluminescence biosensing and imaging platform for detection of H2O2 and PSA. Biosens Bioelectron. 2023;232:115315.
Li, H., Cai, Q., Wang, J., & Jie, G. (2023). Versatile FeMoOv nanozyme bipolar electrode electrochemiluminescence biosensing and imaging platform for detection of H2O2 and PSA. Biosensors & Bioelectronics, 232, 115315. https://doi.org/10.1016/j.bios.2023.115315
Li H, et al. Versatile FeMoOv Nanozyme Bipolar Electrode Electrochemiluminescence Biosensing and Imaging Platform for Detection of H2O2 and PSA. Biosens Bioelectron. 2023 Jul 15;232:115315. PubMed PMID: 37068419.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Versatile FeMoOv nanozyme bipolar electrode electrochemiluminescence biosensing and imaging platform for detection of H2O2 and PSA. AU - Li,Hongkun, AU - Cai,Qianqian, AU - Wang,Jianru, AU - Jie,Guifen, Y1 - 2023/04/13/ PY - 2022/12/31/received PY - 2023/03/17/revised PY - 2023/04/08/accepted PY - 2023/5/1/medline PY - 2023/4/18/pubmed PY - 2023/4/17/entrez KW - Bipolar electrode KW - Dual target KW - ECL KW - Image KW - Nanozyme SP - 115315 EP - 115315 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 232 N2 - In this work, a unique FeMoOv nanozyme-bipolar electrode (NM-BPE) electrochemiluminescence (ECL) biosensing and imaging platform was proposed for the first time to realize sensitive detection of target hydrogen peroxide (H2O2) and prostate specific antigen (PSA). Considering the advantage that the cathode and anode poles of the bipolar electrode (BPE) can be modified respectively, this work was carried out using anode equipped with ECL reagent bipyridine ruthenium (Ru(bpy)3[2+]), and cathode equipped with the Fe-doped molybdenum oxide/Au nanoparticles (FeMoOv/AuNPs) with excellent peroxidase (POD) and catalase (CAT)-like activity. Because FeMoOv/AuNPs show efficient enzyme catalysis effect and can greatly promote the decomposition of H2O2, thus the electron transfer rate in the NM-BPE system would be much accelerated to enhance the ECL signal of Ru(bpy)3[2+]. Based on this principle, this work not only realized sensitive detection of H2O2, but also ingeniously designed an sandwich immunosensor using FeMoOv/AuNPs as recognition probe to mediate the ECL response on the anode, achieving highly sensitive detection of PSA. Furthermore, a unique mobile phone ECL imaging system was developed for assay of PSA at different concentrations, which opened a new portable imaging sensing device for bioassays. This work was the first time to combine nanozymes with bipolar electrodes for ECL analysis and imaging, which not only broadened the applications of nanozymes, but also pioneered the new joint ECL research technique of bipolar electrode and ECL imaging in bioassays, showing great application prospect for multiple detection of proteins, nucleic acids and cancer cells. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/37068419/Versatile_FeMoOv_nanozyme_bipolar_electrode_electrochemiluminescence_biosensing_and_imaging_platform_for_detection_of_H2O2_and_PSA_ DB - PRIME DP - Unbound Medicine ER -