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Biological Activity and Component Analyses of Chamaecyparis obtusa Leaf Extract: Evaluation of Antiwrinkle and Cell Protection Effects in UVA-Irradiated Cells.
Medicina (Kaunas). 2023 Apr 13; 59(4)M

Abstract

Background and Objectives: Chamaecyparis obtusa (C. obtuse) extract has been used as a folk medicinal remedy in East Asian countries to alleviate inflammation and prevent allergies. Active oxygen causes skin aging and leads to skin cell and tissue damage. Extensive research has been conducted to control active oxygen generation to prevent skin aging. We evaluated the antioxidant activity and antiwrinkle effect of C. obtusa extract to determine its potential as a cosmetic material. Materials and Methods: The antioxidant activity of a 70% ethanol extract of C. obtusa (COE 70) and a water extract of C. obtusa (COW) was determined using 2,2-diphenyl-1-picrylhydrazy (DPPH) scavenging, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS[+]) scavenging, superoxide dismutase-like activity, xanthine oxidase inhibition, and ferric-reducing antioxidant power assays. The effective concentration of the extracts was determined using the methyl thiazolyl tetrazolium assay to evaluate their toxicity. The effects of COE 70 on the production of matrix metalloproteinases (MMPs) and procollagen, and expression of activated cytokines, interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α), in UVA-irradiated fibroblasts were determined using quantitative real-time PCR. Additionally, quercitrin, amentoflavone, hinokiflavone, and myricetin concentrations in COE 70 were determined using high-pressure high-performance liquid chromatography. Results: COE 70 had higher polyphenol and flavonoid concentrations than COW and exhibited an excellent antioxidant effect. COE 70 suppressed UVA-induced fibroblast death by 21.3% at 25 µg/mL. It also increased MMP-1, MMP-3, TNF-α, and IL-6 mRNA levels at 5-25 µg/mL compared with those in control UVA-irradiated fibroblasts. Moreover, mRNA levels of collagen type I and superoxide dismutase significantly increased, indicating the antiwrinkle and anti-inflammatory effects of the extract. Among the COE 70 components, quercitrin concentration was the highest; hence, quercitrin could be an active ingredient. Conclusions: COE 70 could be used as a natural antioxidant and antiwrinkle agent.

Authors+Show Affiliations

Convergence Research Center for Smart Healthcare of KS R & DB Foundation, Kyungsung University, Busan 48434, Republic of Korea.Department of Pharmaceutical Engineering, Daegu Catholic University, Gyeongsan-si 38430, Republic of Korea.College of Pharmacy, Kyungsung University, Busan 48434, Republic of Korea.Department of Cosmetic Science, Kyungsung University, Busan 48434, Republic of Korea.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

37109713

Citation

Jang, Young-Ah, et al. "Biological Activity and Component Analyses of Chamaecyparis Obtusa Leaf Extract: Evaluation of Antiwrinkle and Cell Protection Effects in UVA-Irradiated Cells." Medicina (Kaunas, Lithuania), vol. 59, no. 4, 2023.
Jang YA, Kim SG, Kim HK, et al. Biological Activity and Component Analyses of Chamaecyparis obtusa Leaf Extract: Evaluation of Antiwrinkle and Cell Protection Effects in UVA-Irradiated Cells. Medicina (Kaunas). 2023;59(4).
Jang, Y. A., Kim, S. G., Kim, H. K., & Lee, J. T. (2023). Biological Activity and Component Analyses of Chamaecyparis obtusa Leaf Extract: Evaluation of Antiwrinkle and Cell Protection Effects in UVA-Irradiated Cells. Medicina (Kaunas, Lithuania), 59(4). https://doi.org/10.3390/medicina59040755
Jang YA, et al. Biological Activity and Component Analyses of Chamaecyparis Obtusa Leaf Extract: Evaluation of Antiwrinkle and Cell Protection Effects in UVA-Irradiated Cells. Medicina (Kaunas). 2023 Apr 13;59(4) PubMed PMID: 37109713.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biological Activity and Component Analyses of Chamaecyparis obtusa Leaf Extract: Evaluation of Antiwrinkle and Cell Protection Effects in UVA-Irradiated Cells. AU - Jang,Young-Ah, AU - Kim,Se-Gie, AU - Kim,Hye-Kyung, AU - Lee,Jin-Tae, Y1 - 2023/04/13/ PY - 2023/01/16/received PY - 2023/03/31/revised PY - 2023/04/03/accepted PY - 2023/5/1/medline PY - 2023/4/28/pubmed PY - 2023/4/28/entrez KW - Chamaecyparis obtusa KW - antioxidant KW - antiwrinkle KW - fibroblast KW - flavonoid KW - quercitrin JF - Medicina (Kaunas, Lithuania) JO - Medicina (Kaunas) VL - 59 IS - 4 N2 - Background and Objectives: Chamaecyparis obtusa (C. obtuse) extract has been used as a folk medicinal remedy in East Asian countries to alleviate inflammation and prevent allergies. Active oxygen causes skin aging and leads to skin cell and tissue damage. Extensive research has been conducted to control active oxygen generation to prevent skin aging. We evaluated the antioxidant activity and antiwrinkle effect of C. obtusa extract to determine its potential as a cosmetic material. Materials and Methods: The antioxidant activity of a 70% ethanol extract of C. obtusa (COE 70) and a water extract of C. obtusa (COW) was determined using 2,2-diphenyl-1-picrylhydrazy (DPPH) scavenging, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS[+]) scavenging, superoxide dismutase-like activity, xanthine oxidase inhibition, and ferric-reducing antioxidant power assays. The effective concentration of the extracts was determined using the methyl thiazolyl tetrazolium assay to evaluate their toxicity. The effects of COE 70 on the production of matrix metalloproteinases (MMPs) and procollagen, and expression of activated cytokines, interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α), in UVA-irradiated fibroblasts were determined using quantitative real-time PCR. Additionally, quercitrin, amentoflavone, hinokiflavone, and myricetin concentrations in COE 70 were determined using high-pressure high-performance liquid chromatography. Results: COE 70 had higher polyphenol and flavonoid concentrations than COW and exhibited an excellent antioxidant effect. COE 70 suppressed UVA-induced fibroblast death by 21.3% at 25 µg/mL. It also increased MMP-1, MMP-3, TNF-α, and IL-6 mRNA levels at 5-25 µg/mL compared with those in control UVA-irradiated fibroblasts. Moreover, mRNA levels of collagen type I and superoxide dismutase significantly increased, indicating the antiwrinkle and anti-inflammatory effects of the extract. Among the COE 70 components, quercitrin concentration was the highest; hence, quercitrin could be an active ingredient. Conclusions: COE 70 could be used as a natural antioxidant and antiwrinkle agent. SN - 1648-9144 UR - https://www.unboundmedicine.com/medline/citation/37109713/Biological_Activity_and_Component_Analyses_of_Chamaecyparis_obtusa_Leaf_Extract:_Evaluation_of_Antiwrinkle_and_Cell_Protection_Effects_in_UVA_Irradiated_Cells_ DB - PRIME DP - Unbound Medicine ER -