Tags

Type your tag names separated by a space and hit enter

The chemical mechanism of sheep liver 6-phosphogluconate dehydrogenase. A Schiff-base intermediate is not involved.
Biochem J. 1986 Mar 15; 234(3):671-7.BJ

Abstract

[2-18O]Ribulose 5-phosphate was prepared and shown to be converted enzymically by 6-phosphogluconate dehydrogenase from sheep liver into 6-phosphogluconate with complete retention of the heavy isotope. This finding unequivocally excludes the possibility of a Schiff-base mechanism for the enzyme. The involvement of metal ions has already been excluded, and other possible mechanisms are discussed. The enzyme was purified by an improved large-scale procedure, which is briefly described.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3718491

Citation

Topham, C M., and K Dalziel. "The Chemical Mechanism of Sheep Liver 6-phosphogluconate Dehydrogenase. a Schiff-base Intermediate Is Not Involved." The Biochemical Journal, vol. 234, no. 3, 1986, pp. 671-7.
Topham CM, Dalziel K. The chemical mechanism of sheep liver 6-phosphogluconate dehydrogenase. A Schiff-base intermediate is not involved. Biochem J. 1986;234(3):671-7.
Topham, C. M., & Dalziel, K. (1986). The chemical mechanism of sheep liver 6-phosphogluconate dehydrogenase. A Schiff-base intermediate is not involved. The Biochemical Journal, 234(3), 671-7.
Topham CM, Dalziel K. The Chemical Mechanism of Sheep Liver 6-phosphogluconate Dehydrogenase. a Schiff-base Intermediate Is Not Involved. Biochem J. 1986 Mar 15;234(3):671-7. PubMed PMID: 3718491.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The chemical mechanism of sheep liver 6-phosphogluconate dehydrogenase. A Schiff-base intermediate is not involved. AU - Topham,C M, AU - Dalziel,K, PY - 1986/3/15/pubmed PY - 1986/3/15/medline PY - 1986/3/15/entrez SP - 671 EP - 7 JF - The Biochemical journal JO - Biochem. J. VL - 234 IS - 3 N2 - [2-18O]Ribulose 5-phosphate was prepared and shown to be converted enzymically by 6-phosphogluconate dehydrogenase from sheep liver into 6-phosphogluconate with complete retention of the heavy isotope. This finding unequivocally excludes the possibility of a Schiff-base mechanism for the enzyme. The involvement of metal ions has already been excluded, and other possible mechanisms are discussed. The enzyme was purified by an improved large-scale procedure, which is briefly described. SN - 0264-6021 UR - https://www.unboundmedicine.com/medline/citation/3718491/The_chemical_mechanism_of_sheep_liver_6_phosphogluconate_dehydrogenase__A_Schiff_base_intermediate_is_not_involved_ L2 - https://portlandpress.com/biochemj/article-lookup/doi/10.1042/bj2340671 DB - PRIME DP - Unbound Medicine ER -