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Golden berry leaf extract containing withanolides suppresses TNF-α and IL-17 induced IL-6 expression in HeLa Cells.
Biosci Biotechnol Biochem. 2023 Aug 23; 87(9):972-980.BB

Abstract

Inflammation, characterized by the overexpression of IL-6 in various tissues, has been reported as a symptom of coronavirus disease 2019. In this study, we established an experimental system for overexpression of IL-6 in HeLa cells stimulated by TNF-α and IL-17, along with identification of anti-inflammatory materials and components from local agricultural, forestry, and fishery resources. We constructed a library of extracts from natural sources, of which 111 samples were evaluated for their anti-inflammatory activities. The MeOH extract of Golden Berry (Physalis peruviana L) leaf was found to exhibit strong anti-inflammatory properties (IC50 = 4.97 µg/mL). Preparative chromatography identified two active constituents, 4β-hydroxywithanolide E (4β-HWE) (IC50 = 183 nM) and withanolide E (WE) (IC50 = 65.1 nM). Withanolides are known anti-inflammatory ingredients of Withania somnifera, an Ayurvedic herbal medicine. P. peruviana leaves containing 4β-HWE and WE should be considered as useful natural resources for anti-inflammatory products.

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Authors+Show Affiliations

Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.

Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.

Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.

Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.

Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.

Department of Biological Chemistry and Food Science, Faculty of Agriculture, Iwate University, Morioka, Japan.

Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.

MeSH

HumansCOVID-19HeLa CellsInterleukin-17Interleukin-6Plant ExtractsTumor Necrosis Factor-alphaWithanolidesPhysalisPlant Leaves

Pub Type(s)

Journal Article

Language

eng

PubMed ID

37279446

Citation

Yano, Akira, et al. "Golden Berry Leaf Extract Containing Withanolides Suppresses TNF-α and IL-17 Induced IL-6 Expression in HeLa Cells." Bioscience, Biotechnology, and Biochemistry, vol. 87, no. 9, 2023, pp. 972-980.

Yano A, Yuki S, Shiraishi A, et al. Golden berry leaf extract containing withanolides suppresses TNF-α and IL-17 induced IL-6 expression in HeLa Cells. Biosci Biotechnol Biochem. 2023;87(9):972-980.

Yano, A., Yuki, S., Shiraishi, A., Hakozaki, M., Kanno, Y., Kimura, K. I., & Uesugi, S. (2023). Golden berry leaf extract containing withanolides suppresses TNF-α and IL-17 induced IL-6 expression in HeLa Cells. Bioscience, Biotechnology, and Biochemistry, 87(9), 972-980. https://doi.org/10.1093/bbb/zbad070

Yano A, et al. Golden Berry Leaf Extract Containing Withanolides Suppresses TNF-α and IL-17 Induced IL-6 Expression in HeLa Cells. Biosci Biotechnol Biochem. 2023 Aug 23;87(9):972-980. PubMed PMID: 37279446.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Golden berry leaf extract containing withanolides suppresses TNF-α and IL-17 induced IL-6 expression in HeLa Cells. AU - Yano,Akira, AU - Yuki,Sayaka, AU - Shiraishi,Akiko, AU - Hakozaki,Mayuka, AU - Kanno,Yuko, AU - Kimura,Ken-Ichi, AU - Uesugi,Shota, PY - 2023/03/28/received PY - 2023/05/29/accepted PY - 2023/8/24/medline PY - 2023/6/6/pubmed PY - 2023/6/6/entrez KW - Physalis peruviana L KW - 4β-Hydroxywithanolide E KW - IL-6 KW - Withanolide E SP - 972 EP - 980 JF - Bioscience, biotechnology, and biochemistry JO - Biosci Biotechnol Biochem VL - 87 IS - 9 N2 - Inflammation, characterized by the overexpression of IL-6 in various tissues, has been reported as a symptom of coronavirus disease 2019. In this study, we established an experimental system for overexpression of IL-6 in HeLa cells stimulated by TNF-α and IL-17, along with identification of anti-inflammatory materials and components from local agricultural, forestry, and fishery resources. We constructed a library of extracts from natural sources, of which 111 samples were evaluated for their anti-inflammatory activities. The MeOH extract of Golden Berry (Physalis peruviana L) leaf was found to exhibit strong anti-inflammatory properties (IC50 = 4.97 µg/mL). Preparative chromatography identified two active constituents, 4β-hydroxywithanolide E (4β-HWE) (IC50 = 183 nM) and withanolide E (WE) (IC50 = 65.1 nM). Withanolides are known anti-inflammatory ingredients of Withania somnifera, an Ayurvedic herbal medicine. P. peruviana leaves containing 4β-HWE and WE should be considered as useful natural resources for anti-inflammatory products. SN - 1347-6947 UR - https://www.unboundmedicine.com/medline/citation/37279446/Golden_berry_leaf_extract_containing_withanolides_suppresses_TNF_α_and_IL_17_induced_IL_6_expression_in_HeLa_Cells_ DB - PRIME DP - Unbound Medicine ER -