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Detection of inherited adenylosuccinase deficiency by two dimensional thin layer chromatography of urinary imidazoles.
Adv Exp Med Biol 1986; 195 Pt A:21-5AE

Abstract

Patients with inherited adenylosuccinase deficiency excrete large quantities of succinyloaminoimidazolecarboxamide riboside (SAICAR) and succinyloadenosine (SAdo). A two dimensional thin layer chromatography method for the detection of SAICAR is described. The method consists of 1: isolation of imidazoles with a cation exchange resin; 2: tlc on cellulose plates, solvent I: isopropanol-ammonia 10% (4:1) and II: butanol-acetic acid-water (4:1:1); detection with Pauly reagent. SAICAR gives rise to an isolated spot with a characteristic bluish color. Also a simple one dimensional thin layer chromatography method for screening of high risk populations is given. Four new cases could be diagnosed. Clinical and chemical data, including concentrations of SAICAR and SAdo in urine, plasma and cerebrospinal fluid, determined by column chromatography, are presented.

Authors

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Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

3728154

Citation

Wadman, S K., et al. "Detection of Inherited Adenylosuccinase Deficiency By Two Dimensional Thin Layer Chromatography of Urinary Imidazoles." Advances in Experimental Medicine and Biology, vol. 195 Pt A, 1986, pp. 21-5.
Wadman SK, de Bree PK, Duran M, et al. Detection of inherited adenylosuccinase deficiency by two dimensional thin layer chromatography of urinary imidazoles. Adv Exp Med Biol. 1986;195 Pt A:21-5.
Wadman, S. K., de Bree, P. K., Duran, M., & de Jonge, H. F. (1986). Detection of inherited adenylosuccinase deficiency by two dimensional thin layer chromatography of urinary imidazoles. Advances in Experimental Medicine and Biology, 195 Pt A, pp. 21-5.
Wadman SK, et al. Detection of Inherited Adenylosuccinase Deficiency By Two Dimensional Thin Layer Chromatography of Urinary Imidazoles. Adv Exp Med Biol. 1986;195 Pt A:21-5. PubMed PMID: 3728154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of inherited adenylosuccinase deficiency by two dimensional thin layer chromatography of urinary imidazoles. AU - Wadman,S K, AU - de Bree,P K, AU - Duran,M, AU - de Jonge,H F, PY - 1986/1/1/pubmed PY - 1986/1/1/medline PY - 1986/1/1/entrez SP - 21 EP - 5 JF - Advances in experimental medicine and biology JO - Adv. Exp. Med. Biol. VL - 195 Pt A N2 - Patients with inherited adenylosuccinase deficiency excrete large quantities of succinyloaminoimidazolecarboxamide riboside (SAICAR) and succinyloadenosine (SAdo). A two dimensional thin layer chromatography method for the detection of SAICAR is described. The method consists of 1: isolation of imidazoles with a cation exchange resin; 2: tlc on cellulose plates, solvent I: isopropanol-ammonia 10% (4:1) and II: butanol-acetic acid-water (4:1:1); detection with Pauly reagent. SAICAR gives rise to an isolated spot with a characteristic bluish color. Also a simple one dimensional thin layer chromatography method for screening of high risk populations is given. Four new cases could be diagnosed. Clinical and chemical data, including concentrations of SAICAR and SAdo in urine, plasma and cerebrospinal fluid, determined by column chromatography, are presented. SN - 0065-2598 UR - https://www.unboundmedicine.com/medline/citation/3728154/Detection_of_inherited_adenylosuccinase_deficiency_by_two_dimensional_thin_layer_chromatography_of_urinary_imidazoles_ L2 - http://www.diseaseinfosearch.org/result/230 DB - PRIME DP - Unbound Medicine ER -