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Rapid development of wheat-Dasypyrum villosum compensating translocations resistant to powdery mildew using a triple marker strategy conducted on a large ph1b-induced population.
Theor Appl Genet. 2023 Jun 09; 136(7):148.TA

Abstract

KEY MESSAGE

Twenty-two compensating wheat-Dasypyrum villosum translocations carrying the powdery mildew resistance gene PmV were developed using a triple marker selection strategy in a large homozygous ph1bph1b population. Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive wheat disease in China. Currently, nearly all resistant varieties grown in the middle and lower reaches of the Yangtze River carry Pm21 which is present in a wheat-Dasypyrum villosum T6V#2S·6AL translocation. Its widespread use poses a strong risk of loss of effectiveness if the pathogen were to change. PmV, a Pm21 homolog carried by a wheat-D. villosum T6V#4S·6DL translocation, is also resistant to powdery mildew but is less transmittable and exploited in cultivars. To utilize PmV more effectively, a new recombinant translocation T6V#4S-6V#2S·6AL carrying PmV with a higher transmission rate was used as a basic material for inducing smaller alien translocations. A locally adapted ph1b-carrying line, Yangmai 23-ph1b, was crossed with T6V#4S-6V#2S·6AL to generate a homozygous ph1bph1b population of 6300 F3 individuals. A modified triple marker strategy based on three co-dominant markers including the functional marker MBH1 for PmV in combination with distal and proximal markers 6VS-GX4 and 6VS-GX17, respectively, was used to screen for new recombinants efficiently. Forty-eight compensating translocations were identified, 22 of which carried PmV. Two translocation lines, Dv6T25 with the shortest distal segment carrying PmV and Dv6T31 with the shortest proximal segment carrying PmV were identified, both expressed normal transmission and therefore could promote PmV in wheat breeding. This work exemplifies a model for rapid development of wheat-alien compensating translocations.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wan W
Key Laboratory of Wheat Biology and Genetic Improvement on Low and Middle Yangtze River Valley Wheat Region (Ministry of Agriculture), Yangzhou Academy of Agricultural Sciences, Yangzhou, 225007, Jiangsu, China.
Zhao R
Key Laboratory of Wheat Biology and Genetic Improvement on Low and Middle Yangtze River Valley Wheat Region (Ministry of Agriculture), Yangzhou Academy of Agricultural Sciences, Yangzhou, 225007, Jiangsu, China. zhaorh86@163.com.
Chen T
Key Laboratory of Wheat Biology and Genetic Improvement on Low and Middle Yangtze River Valley Wheat Region (Ministry of Agriculture), Yangzhou Academy of Agricultural Sciences, Yangzhou, 225007, Jiangsu, China.
Wang L
Key Laboratory of Wheat Biology and Genetic Improvement on Low and Middle Yangtze River Valley Wheat Region (Ministry of Agriculture), Yangzhou Academy of Agricultural Sciences, Yangzhou, 225007, Jiangsu, China.
Zhang X
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
Li H
Yangzhou Polytechnic College, Yangzhou, 225007, Jiangsu, China.
Wang X
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
Bie T
Key Laboratory of Wheat Biology and Genetic Improvement on Low and Middle Yangtze River Valley Wheat Region (Ministry of Agriculture), Yangzhou Academy of Agricultural Sciences, Yangzhou, 225007, Jiangsu, China. bietd@126.com.

MeSH

HumansTriticumPlant BreedingGenes, PlantPoaceaeTranslocation, GeneticPlant DiseasesDisease Resistance

Pub Type(s)

Journal Article

Language

eng

PubMed ID

37294325

Citation

Wan, Wentao, et al. "Rapid Development of wheat-Dasypyrum Villosum Compensating Translocations Resistant to Powdery Mildew Using a Triple Marker Strategy Conducted On a Large Ph1b-induced Population." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 136, no. 7, 2023, p. 148.
Wan W, Zhao R, Chen T, et al. Rapid development of wheat-Dasypyrum villosum compensating translocations resistant to powdery mildew using a triple marker strategy conducted on a large ph1b-induced population. Theor Appl Genet. 2023;136(7):148.
Wan, W., Zhao, R., Chen, T., Wang, L., Zhang, X., Li, H., Wang, X., & Bie, T. (2023). Rapid development of wheat-Dasypyrum villosum compensating translocations resistant to powdery mildew using a triple marker strategy conducted on a large ph1b-induced population. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 136(7), 148. https://doi.org/10.1007/s00122-023-04393-z
Wan W, et al. Rapid Development of wheat-Dasypyrum Villosum Compensating Translocations Resistant to Powdery Mildew Using a Triple Marker Strategy Conducted On a Large Ph1b-induced Population. Theor Appl Genet. 2023 Jun 9;136(7):148. PubMed PMID: 37294325.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid development of wheat-Dasypyrum villosum compensating translocations resistant to powdery mildew using a triple marker strategy conducted on a large ph1b-induced population. AU - Wan,Wentao, AU - Zhao,Renhui, AU - Chen,Tiantian, AU - Wang,Ling, AU - Zhang,Xu, AU - Li,Haifeng, AU - Wang,Xiue, AU - Bie,Tongde, Y1 - 2023/06/09/ PY - 2023/03/08/received PY - 2023/05/29/accepted PY - 2023/6/12/medline PY - 2023/6/9/pubmed PY - 2023/6/9/entrez SP - 148 EP - 148 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 136 IS - 7 N2 - KEY MESSAGE: Twenty-two compensating wheat-Dasypyrum villosum translocations carrying the powdery mildew resistance gene PmV were developed using a triple marker selection strategy in a large homozygous ph1bph1b population. Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a destructive wheat disease in China. Currently, nearly all resistant varieties grown in the middle and lower reaches of the Yangtze River carry Pm21 which is present in a wheat-Dasypyrum villosum T6V#2S·6AL translocation. Its widespread use poses a strong risk of loss of effectiveness if the pathogen were to change. PmV, a Pm21 homolog carried by a wheat-D. villosum T6V#4S·6DL translocation, is also resistant to powdery mildew but is less transmittable and exploited in cultivars. To utilize PmV more effectively, a new recombinant translocation T6V#4S-6V#2S·6AL carrying PmV with a higher transmission rate was used as a basic material for inducing smaller alien translocations. A locally adapted ph1b-carrying line, Yangmai 23-ph1b, was crossed with T6V#4S-6V#2S·6AL to generate a homozygous ph1bph1b population of 6300 F3 individuals. A modified triple marker strategy based on three co-dominant markers including the functional marker MBH1 for PmV in combination with distal and proximal markers 6VS-GX4 and 6VS-GX17, respectively, was used to screen for new recombinants efficiently. Forty-eight compensating translocations were identified, 22 of which carried PmV. Two translocation lines, Dv6T25 with the shortest distal segment carrying PmV and Dv6T31 with the shortest proximal segment carrying PmV were identified, both expressed normal transmission and therefore could promote PmV in wheat breeding. This work exemplifies a model for rapid development of wheat-alien compensating translocations. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/37294325/Rapid_development_of_wheat_Dasypyrum_villosum_compensating_translocations_resistant_to_powdery_mildew_using_a_triple_marker_strategy_conducted_on_a_large_ph1b_induced_population_ DB - PRIME DP - Unbound Medicine ER -