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Androgen regulation of the androgen receptor of the quail uropygial gland: application of a [3H]mibolerone exchange assay.
J Endocrinol. 1986 Jun; 109(3):299-306.JE

Abstract

An exchange assay for androgen receptors in the quail uropygial gland using [3H]mibolerone was established. The most efficient exchange conditions were 3 days of incubation at 15 degrees C. Under these conditions, androgen receptors were stable in the presence of sodium molybdate, and the exchange of [3H]mibolerone with endogenous testosterone bound to cytosolic or nuclear androgen receptors was maximal. Less than 5% of [3H]mibolerone-binding sites occurred in the extracted nuclear pellets. Using this exchange technique, it was shown that androgen receptors in the uropygial gland of photostimulated male quail or castrated quail treated with testosterone were activated and that their concentrations in both cytosolic and nuclear fractions were increased. These results confirm the androgen dependency of the quail uropygial gland, and show that it is an organ which can be used as a model for the study of androgen action in sebaceous glands.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

3734664

Citation

Amet, Y, et al. "Androgen Regulation of the Androgen Receptor of the Quail Uropygial Gland: Application of a [3H]mibolerone Exchange Assay." The Journal of Endocrinology, vol. 109, no. 3, 1986, pp. 299-306.
Amet Y, Abalain JH, di Stefano S, et al. Androgen regulation of the androgen receptor of the quail uropygial gland: application of a [3H]mibolerone exchange assay. J Endocrinol. 1986;109(3):299-306.
Amet, Y., Abalain, J. H., di Stefano, S., Daniel, J. Y., Tea, K., Floch, H. H., & Robel, P. (1986). Androgen regulation of the androgen receptor of the quail uropygial gland: application of a [3H]mibolerone exchange assay. The Journal of Endocrinology, 109(3), 299-306.
Amet Y, et al. Androgen Regulation of the Androgen Receptor of the Quail Uropygial Gland: Application of a [3H]mibolerone Exchange Assay. J Endocrinol. 1986;109(3):299-306. PubMed PMID: 3734664.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Androgen regulation of the androgen receptor of the quail uropygial gland: application of a [3H]mibolerone exchange assay. AU - Amet,Y, AU - Abalain,J H, AU - di Stefano,S, AU - Daniel,J Y, AU - Tea,K, AU - Floch,H H, AU - Robel,P, PY - 1986/6/1/pubmed PY - 1986/6/1/medline PY - 1986/6/1/entrez SP - 299 EP - 306 JF - The Journal of endocrinology JO - J. Endocrinol. VL - 109 IS - 3 N2 - An exchange assay for androgen receptors in the quail uropygial gland using [3H]mibolerone was established. The most efficient exchange conditions were 3 days of incubation at 15 degrees C. Under these conditions, androgen receptors were stable in the presence of sodium molybdate, and the exchange of [3H]mibolerone with endogenous testosterone bound to cytosolic or nuclear androgen receptors was maximal. Less than 5% of [3H]mibolerone-binding sites occurred in the extracted nuclear pellets. Using this exchange technique, it was shown that androgen receptors in the uropygial gland of photostimulated male quail or castrated quail treated with testosterone were activated and that their concentrations in both cytosolic and nuclear fractions were increased. These results confirm the androgen dependency of the quail uropygial gland, and show that it is an organ which can be used as a model for the study of androgen action in sebaceous glands. SN - 0022-0795 UR - https://www.unboundmedicine.com/medline/citation/3734664/Androgen_regulation_of_the_androgen_receptor_of_the_quail_uropygial_gland:_application_of_a_[3H]mibolerone_exchange_assay_ L2 - https://joe.bioscientifica.com/doi/10.1677/joe.0.1090299 DB - PRIME DP - Unbound Medicine ER -