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[Comparative study of chicken liver xanthine dehydrogenase and bovine liver xanthine oxidase. dehydrogenase activity of xanthine oxidase (author's transl)].
Rev Esp Fisiol. 1979 Mar; 35(1):51-62.RE

Abstract

A method to purify bovine liver xanthine oxidase in described, with which samples of 256-fold specific activity with respect to the initial homogenate are obtained. Bovine liver xanthine oxidase and chicken liver xanthine dehydrogenase with oxygen as electron acceptor exhibit similar profile in pKM and log V versus pH plots. With NAD+ as electron acceptor a different profile in the pKM xanthine plot is obtained for chicken liver xanthine dehydrogenase. However three inflection points at the same pH values appear in all plots. Both enzymes are irreversibly inhibited by pCMB and reversibly by N-ethylmaleimide and by iodoacetamide, with competitive and uncompetitive type inhibitions respectively. These results suggest that NAD+ alters the enzymatic action since its binding to the enzyme antecedes the binding of xanthine to the xanthine oxidase molecule, without undergoing itself any modification. 0.15 M DDT of DTE treatment of bovine liver xanthine oxidase gives to the enzyme a permanent activity with NAD+ without modifying its activity with oxygen. The enzyme thus treated produces parallel straight lines in Lineweaver-Burk plots.

Authors

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Pub Type(s)

Comparative Study
English Abstract
Journal Article

Language

spa

PubMed ID

37557

Citation

Canela, E, and J Bozal. "[Comparative Study of Chicken Liver Xanthine Dehydrogenase and Bovine Liver Xanthine Oxidase. Dehydrogenase Activity of Xanthine Oxidase (author's Transl)]." Revista Espanola De Fisiologia, vol. 35, no. 1, 1979, pp. 51-62.
Canela E, Bozal J. [Comparative study of chicken liver xanthine dehydrogenase and bovine liver xanthine oxidase. dehydrogenase activity of xanthine oxidase (author's transl)]. Rev Esp Fisiol. 1979;35(1):51-62.
Canela, E., & Bozal, J. (1979). [Comparative study of chicken liver xanthine dehydrogenase and bovine liver xanthine oxidase. dehydrogenase activity of xanthine oxidase (author's transl)]. Revista Espanola De Fisiologia, 35(1), 51-62.
Canela E, Bozal J. [Comparative Study of Chicken Liver Xanthine Dehydrogenase and Bovine Liver Xanthine Oxidase. Dehydrogenase Activity of Xanthine Oxidase (author's Transl)]. Rev Esp Fisiol. 1979;35(1):51-62. PubMed PMID: 37557.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Comparative study of chicken liver xanthine dehydrogenase and bovine liver xanthine oxidase. dehydrogenase activity of xanthine oxidase (author's transl)]. AU - Canela,E, AU - Bozal,J, PY - 1979/3/1/pubmed PY - 1979/3/1/medline PY - 1979/3/1/entrez SP - 51 EP - 62 JF - Revista espanola de fisiologia JO - Rev Esp Fisiol VL - 35 IS - 1 N2 - A method to purify bovine liver xanthine oxidase in described, with which samples of 256-fold specific activity with respect to the initial homogenate are obtained. Bovine liver xanthine oxidase and chicken liver xanthine dehydrogenase with oxygen as electron acceptor exhibit similar profile in pKM and log V versus pH plots. With NAD+ as electron acceptor a different profile in the pKM xanthine plot is obtained for chicken liver xanthine dehydrogenase. However three inflection points at the same pH values appear in all plots. Both enzymes are irreversibly inhibited by pCMB and reversibly by N-ethylmaleimide and by iodoacetamide, with competitive and uncompetitive type inhibitions respectively. These results suggest that NAD+ alters the enzymatic action since its binding to the enzyme antecedes the binding of xanthine to the xanthine oxidase molecule, without undergoing itself any modification. 0.15 M DDT of DTE treatment of bovine liver xanthine oxidase gives to the enzyme a permanent activity with NAD+ without modifying its activity with oxygen. The enzyme thus treated produces parallel straight lines in Lineweaver-Burk plots. SN - 0034-9402 UR - https://www.unboundmedicine.com/medline/citation/37557/[Comparative_study_of_chicken_liver_xanthine_dehydrogenase_and_bovine_liver_xanthine_oxidase__dehydrogenase_activity_of_xanthine_oxidase__author's_transl_]_ DB - PRIME DP - Unbound Medicine ER -