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3D DNAzyme walker based electrochemical biosensor for attomolar level microRNA-155 detection.
Anal Chim Acta. 2023 Oct 02; 1276:341642.AC

Abstract

Herein, an ultrasensitive electrochemical biosensor for microRNA-155 (miR-155) detection based on the powerful catalytic and continuous walking signal amplification capability of 3D DNAzyme walker and the gold nanoparticles/graphene aerogels carbon fiber paper-based (AuNPs/GAs/CFP) flexible sensing electrode with excellent electrochemical performance was successfully constructed. In a proof-of-concept experiment, in the presence of miR-155, the DNAzyme strands anchored on the streptavidin-modified magnetic beads (MBs) silenced by locked strands can be activated, thus generating the walking arm of the 3D DNAzyme walker. Meanwhile, the substrate strands modified with Fe-MOF-NH2 nanoparticles were evenly distributed on the surface of MBs and served as tracks of the 3D DNAzyme walker. Once the DNAzyme strand was activated, the catalytic site in the substrate strand can be cleaved in the presence of Mn[2+], and a large number of stumps modified with Fe-MOF-NH2 nanoparticles (output@Fe-MOF-NH2) will be generated during the continuous and efficient walking cleavage of the DNAzyme walker, driving the recognition-catalysis-release cycle process for signal amplification. Immediately afterwards, the signal was read out through the base complementary pairing of capture probe (PS) immobilized on the surface of the paper-based flexible sensing electrode AuNPs/GAs/CFP and signal probes output@Fe-MOF-NH2, thus achieving the quantitative detection of miR-155. Under optimal experimental conditions, the designed 3D DNAzyme walker-based biosensor exhibited a relatively lower limit of detection (LOD) of 56.23 aM, with a linear range of 100 aM to 100 nM. Overall, the proposed 3D DNAzyme walker biosensor exhibited good interference and reproducibility, demonstrating a promising future in the field of clinical disease diagnosis.

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Authors+Show Affiliations

Zhao J
Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China.
He C
Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China.
Long Y
Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China.
Lei J
Chongqing Engineering and Technology Research Center of Intelligent Rehabilitation and Eldercare, Chongqing City Management College, Chongqing, 401331, PR China.
Liu H
Chongqing Institute for Food and Drug Control, Chongqing, 401121, PR China.
Hou J
Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China; Postdoctoral Research Station, Chongqing University, Bioengineering College of Chongqing University, Chongqing, 400044, PR China; Chongqing Engineering and Technology Research Center of Intelligent Rehabilitation and Eldercare, Chongqing City Management College, Chongqing, 401331, PR China. Electronic address: houjz89329@163.com.
Hou C
Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China. Electronic address: houcj@cqu.edu.cn.
Huo D
Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China; Chongqing Engineering and Technology Research Center of Intelligent Rehabilitation and Eldercare, Chongqing City Management College, Chongqing, 401331, PR China. Electronic address: huodq@cqu.edu.cn.

MeSH

DNA, CatalyticGoldReproducibility of ResultsMetal NanoparticlesElectrochemical TechniquesBiosensing TechniquesLimit of DetectionMicroRNAs

Pub Type(s)

Journal Article

Language

eng

PubMed ID

37573120

Citation

Zhao, Jiaying, et al. "3D DNAzyme Walker Based Electrochemical Biosensor for Attomolar Level microRNA-155 Detection." Analytica Chimica Acta, vol. 1276, 2023, p. 341642.
Zhao J, He C, Long Y, et al. 3D DNAzyme walker based electrochemical biosensor for attomolar level microRNA-155 detection. Anal Chim Acta. 2023;1276:341642.
Zhao, J., He, C., Long, Y., Lei, J., Liu, H., Hou, J., Hou, C., & Huo, D. (2023). 3D DNAzyme walker based electrochemical biosensor for attomolar level microRNA-155 detection. Analytica Chimica Acta, 1276, 341642. https://doi.org/10.1016/j.aca.2023.341642
Zhao J, et al. 3D DNAzyme Walker Based Electrochemical Biosensor for Attomolar Level microRNA-155 Detection. Anal Chim Acta. 2023 Oct 2;1276:341642. PubMed PMID: 37573120.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 3D DNAzyme walker based electrochemical biosensor for attomolar level microRNA-155 detection. AU - Zhao,Jiaying, AU - He,Congjuan, AU - Long,Yanyi, AU - Lei,Jincan, AU - Liu,Huan, AU - Hou,Jingzhou, AU - Hou,Changjun, AU - Huo,Danqun, Y1 - 2023/07/18/ PY - 2023/04/05/received PY - 2023/07/17/revised PY - 2023/07/18/accepted PY - 2023/8/14/medline PY - 2023/8/13/pubmed PY - 2023/8/12/entrez KW - Carbon paper KW - DNAzyme walker KW - Electrochemical detection KW - Graphene aerogels KW - microRNA SP - 341642 EP - 341642 JF - Analytica chimica acta JO - Anal Chim Acta VL - 1276 N2 - Herein, an ultrasensitive electrochemical biosensor for microRNA-155 (miR-155) detection based on the powerful catalytic and continuous walking signal amplification capability of 3D DNAzyme walker and the gold nanoparticles/graphene aerogels carbon fiber paper-based (AuNPs/GAs/CFP) flexible sensing electrode with excellent electrochemical performance was successfully constructed. In a proof-of-concept experiment, in the presence of miR-155, the DNAzyme strands anchored on the streptavidin-modified magnetic beads (MBs) silenced by locked strands can be activated, thus generating the walking arm of the 3D DNAzyme walker. Meanwhile, the substrate strands modified with Fe-MOF-NH2 nanoparticles were evenly distributed on the surface of MBs and served as tracks of the 3D DNAzyme walker. Once the DNAzyme strand was activated, the catalytic site in the substrate strand can be cleaved in the presence of Mn[2+], and a large number of stumps modified with Fe-MOF-NH2 nanoparticles (output@Fe-MOF-NH2) will be generated during the continuous and efficient walking cleavage of the DNAzyme walker, driving the recognition-catalysis-release cycle process for signal amplification. Immediately afterwards, the signal was read out through the base complementary pairing of capture probe (PS) immobilized on the surface of the paper-based flexible sensing electrode AuNPs/GAs/CFP and signal probes output@Fe-MOF-NH2, thus achieving the quantitative detection of miR-155. Under optimal experimental conditions, the designed 3D DNAzyme walker-based biosensor exhibited a relatively lower limit of detection (LOD) of 56.23 aM, with a linear range of 100 aM to 100 nM. Overall, the proposed 3D DNAzyme walker biosensor exhibited good interference and reproducibility, demonstrating a promising future in the field of clinical disease diagnosis. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/37573120/3D_DNAzyme_walker_based_electrochemical_biosensor_for_attomolar_level_microRNA_155_detection_ DB - PRIME DP - Unbound Medicine ER -