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Specific immunoglobulin M enzyme-linked immunosorbent assay for confirming the diagnosis of measles.
J Clin Microbiol. 1986 Sep; 24(3):391-4.JC

Abstract

An enzyme-linked immunosorbent assay (ELISA) was evaluated for the detection of measles virus-specific immunoglobulin M (IgM) (MIgM). The ELISA was standardized by deriving a seronegative range of values from sera which should not contain MIgM (24 cord sera, 59 sera from immune health care workers, and 47 sera from infants before the administration of measles vaccine). These values were separable from those obtained from individuals convalescing from measles. Twenty sera containing rheumatoid factor were MIgM seronegative. Of 30 acute-phase sera from suspected measles cases, 26 contained MIgM; those that were seronegative were obtained on day 0, 0, 2, or 9. All 25 convalescent-phase samples contained MIgM. Of the 25 paired samples, 22 were IgG positive at the first sampling; 3 of the 22 did not show a rise in IgG titer. The MIgM ELISA can be used for confirming suspected measles cases, often requiring only a single serum specimen.

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Authors

Lievens AW
No affiliation info available
Brunell PA
No affiliation info available

MeSH

Antibodies, ViralAntibody SpecificityDithiothreitolEnzyme-Linked Immunosorbent AssayHumansImmunoglobulin GImmunoglobulin MMeaslesMeasles virus

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

3760134

Citation

Lievens, A W., and P A. Brunell. "Specific Immunoglobulin M Enzyme-linked Immunosorbent Assay for Confirming the Diagnosis of Measles." Journal of Clinical Microbiology, vol. 24, no. 3, 1986, pp. 391-4.
Lievens AW, Brunell PA. Specific immunoglobulin M enzyme-linked immunosorbent assay for confirming the diagnosis of measles. J Clin Microbiol. 1986;24(3):391-4.
Lievens, A. W., & Brunell, P. A. (1986). Specific immunoglobulin M enzyme-linked immunosorbent assay for confirming the diagnosis of measles. Journal of Clinical Microbiology, 24(3), 391-4.
Lievens AW, Brunell PA. Specific Immunoglobulin M Enzyme-linked Immunosorbent Assay for Confirming the Diagnosis of Measles. J Clin Microbiol. 1986;24(3):391-4. PubMed PMID: 3760134.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Specific immunoglobulin M enzyme-linked immunosorbent assay for confirming the diagnosis of measles. AU - Lievens,A W, AU - Brunell,P A, PY - 1986/9/1/pubmed PY - 1986/9/1/medline PY - 1986/9/1/entrez SP - 391 EP - 4 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 24 IS - 3 N2 - An enzyme-linked immunosorbent assay (ELISA) was evaluated for the detection of measles virus-specific immunoglobulin M (IgM) (MIgM). The ELISA was standardized by deriving a seronegative range of values from sera which should not contain MIgM (24 cord sera, 59 sera from immune health care workers, and 47 sera from infants before the administration of measles vaccine). These values were separable from those obtained from individuals convalescing from measles. Twenty sera containing rheumatoid factor were MIgM seronegative. Of 30 acute-phase sera from suspected measles cases, 26 contained MIgM; those that were seronegative were obtained on day 0, 0, 2, or 9. All 25 convalescent-phase samples contained MIgM. Of the 25 paired samples, 22 were IgG positive at the first sampling; 3 of the 22 did not show a rise in IgG titer. The MIgM ELISA can be used for confirming suspected measles cases, often requiring only a single serum specimen. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/3760134/Specific_immunoglobulin_M_enzyme_linked_immunosorbent_assay_for_confirming_the_diagnosis_of_measles_ DB - PRIME DP - Unbound Medicine ER -