Tags

Type your tag names separated by a space and hit enter

Recombinant Cedar Virus: A Henipavirus Reverse Genetics Platform.
Methods Mol Biol. 2023; 2682:73-86.MM

Abstract

The isolation of Cedar virus, a nonpathogenic henipavirus that is closely related to the highly pathogenic Nipah virus and Hendra virus, provides a new platform for henipavirus experimentation and a tool to investigate biological differences among these viruses under less stringent biological containment. Here, we detail a reverse genetics system used to rescue two replication-competent, recombinant Cedar virus variants: a recombinant wild-type Cedar virus and a recombinant Cedar virus that express a green fluorescent protein from an open reading frame inserted between the phosphoprotein and matrix genes. This recombinant Cedar virus platform may be utilized to characterize the determinants of pathogenesis across the henipaviruses, investigate their receptor tropisms, and identify novel pan-henipavirus antivirals safely under biosafety level-2 conditions.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Amaya M
Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA.
Broder CC
Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA.
Laing ED
Department of Microbiology and Immunology, Uniformed Services University, Bethesda, MD, USA. eric.laing@usuhs.edu.

MeSH

HenipavirusReverse GeneticsOrthopoxvirusAntiviral Agents

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

37610574

Citation

Amaya, Moushimi, et al. "Recombinant Cedar Virus: a Henipavirus Reverse Genetics Platform." Methods in Molecular Biology (Clifton, N.J.), vol. 2682, 2023, pp. 73-86.
Amaya M, Broder CC, Laing ED. Recombinant Cedar Virus: A Henipavirus Reverse Genetics Platform. Methods Mol Biol. 2023;2682:73-86.
Amaya, M., Broder, C. C., & Laing, E. D. (2023). Recombinant Cedar Virus: A Henipavirus Reverse Genetics Platform. Methods in Molecular Biology (Clifton, N.J.), 2682, 73-86. https://doi.org/10.1007/978-1-0716-3283-3_5
Amaya M, Broder CC, Laing ED. Recombinant Cedar Virus: a Henipavirus Reverse Genetics Platform. Methods Mol Biol. 2023;2682:73-86. PubMed PMID: 37610574.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Recombinant Cedar Virus: A Henipavirus Reverse Genetics Platform. AU - Amaya,Moushimi, AU - Broder,Christopher C, AU - Laing,Eric D, PY - 2023/8/24/medline PY - 2023/8/23/pubmed PY - 2023/8/23/entrez KW - Cedar virus KW - Henipavirus KW - Reverse genetics KW - Virus plaque assay KW - Virus rescue KW - Virus titration SP - 73 EP - 86 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 2682 N2 - The isolation of Cedar virus, a nonpathogenic henipavirus that is closely related to the highly pathogenic Nipah virus and Hendra virus, provides a new platform for henipavirus experimentation and a tool to investigate biological differences among these viruses under less stringent biological containment. Here, we detail a reverse genetics system used to rescue two replication-competent, recombinant Cedar virus variants: a recombinant wild-type Cedar virus and a recombinant Cedar virus that express a green fluorescent protein from an open reading frame inserted between the phosphoprotein and matrix genes. This recombinant Cedar virus platform may be utilized to characterize the determinants of pathogenesis across the henipaviruses, investigate their receptor tropisms, and identify novel pan-henipavirus antivirals safely under biosafety level-2 conditions. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/37610574/Recombinant_Cedar_Virus:_A_Henipavirus_Reverse_Genetics_Platform_ DB - PRIME DP - Unbound Medicine ER -