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Presynaptic inhibitory muscarinic receptors modulating [3H] acetylcholine release in the rat urinary bladder.
J Pharmacol Exp Ther. 1986 Nov; 239(2):522-8.JP

Abstract

The occurrence of presynaptic muscarinic receptors inhibiting the release of acetylcholine (ACh) from nerve terminals was investigated in the rat urinary bladder. Strips from the extratrigonal area were preincubated with [3H]choline and stimulated at 0.2 Hz. Both [3H]ACh and [3H]choline content were measured in the tissue. The uptake of tritiated choline was prevented by hemicholinium-3. The field stimulation at 2 Hz (360 shocks) produced a release of tritium. Most of the induced outflow was found to be [3H]ACh. Both tetrodotoxin treatment and calcium omission from the medium prevented such an evoked-outflow of tritium. When two electrical stimulations (S1 and S2) at 2 Hz (360 shocks) were carried out at 45-min intervals, an S2/S1 ratio of 0.82 was found. Physostigmine reduced the evoked-release of [3H]ACh whereas atropine increased it in a concentration-dependent manner. Atropine antagonized the inhibitory effect of physostigmine, so that the S2/S1 ratio did not vary significantly from control experiments. Both carbachol and muscarine strongly decreased the [3H]ACh evoked outflow. Muscarine increased the spontaneous outflow of tritium also. These findings suggest that the urinary bladder of the rat is equipped with presynaptic inhibitory muscarinic receptors modulating ACh release from cholinergic postganglionic neurons.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3772807

Citation

D'Agostino, G, et al. "Presynaptic Inhibitory Muscarinic Receptors Modulating [3H] Acetylcholine Release in the Rat Urinary Bladder." The Journal of Pharmacology and Experimental Therapeutics, vol. 239, no. 2, 1986, pp. 522-8.
D'Agostino G, Kilbinger H, Chiari MC, et al. Presynaptic inhibitory muscarinic receptors modulating [3H] acetylcholine release in the rat urinary bladder. J Pharmacol Exp Ther. 1986;239(2):522-8.
D'Agostino, G., Kilbinger, H., Chiari, M. C., & Grana, E. (1986). Presynaptic inhibitory muscarinic receptors modulating [3H] acetylcholine release in the rat urinary bladder. The Journal of Pharmacology and Experimental Therapeutics, 239(2), 522-8.
D'Agostino G, et al. Presynaptic Inhibitory Muscarinic Receptors Modulating [3H] Acetylcholine Release in the Rat Urinary Bladder. J Pharmacol Exp Ther. 1986;239(2):522-8. PubMed PMID: 3772807.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Presynaptic inhibitory muscarinic receptors modulating [3H] acetylcholine release in the rat urinary bladder. AU - D'Agostino,G, AU - Kilbinger,H, AU - Chiari,M C, AU - Grana,E, PY - 1986/11/1/pubmed PY - 1986/11/1/medline PY - 1986/11/1/entrez SP - 522 EP - 8 JF - The Journal of pharmacology and experimental therapeutics JO - J. Pharmacol. Exp. Ther. VL - 239 IS - 2 N2 - The occurrence of presynaptic muscarinic receptors inhibiting the release of acetylcholine (ACh) from nerve terminals was investigated in the rat urinary bladder. Strips from the extratrigonal area were preincubated with [3H]choline and stimulated at 0.2 Hz. Both [3H]ACh and [3H]choline content were measured in the tissue. The uptake of tritiated choline was prevented by hemicholinium-3. The field stimulation at 2 Hz (360 shocks) produced a release of tritium. Most of the induced outflow was found to be [3H]ACh. Both tetrodotoxin treatment and calcium omission from the medium prevented such an evoked-outflow of tritium. When two electrical stimulations (S1 and S2) at 2 Hz (360 shocks) were carried out at 45-min intervals, an S2/S1 ratio of 0.82 was found. Physostigmine reduced the evoked-release of [3H]ACh whereas atropine increased it in a concentration-dependent manner. Atropine antagonized the inhibitory effect of physostigmine, so that the S2/S1 ratio did not vary significantly from control experiments. Both carbachol and muscarine strongly decreased the [3H]ACh evoked outflow. Muscarine increased the spontaneous outflow of tritium also. These findings suggest that the urinary bladder of the rat is equipped with presynaptic inhibitory muscarinic receptors modulating ACh release from cholinergic postganglionic neurons. SN - 0022-3565 UR - https://www.unboundmedicine.com/medline/citation/3772807/Presynaptic_inhibitory_muscarinic_receptors_modulating_[3H]_acetylcholine_release_in_the_rat_urinary_bladder_ L2 - http://jpet.aspetjournals.org/cgi/pmidlookup?view=long&pmid=3772807 DB - PRIME DP - Unbound Medicine ER -