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Isolation and characterization of replication-deficient mutants of ColE1 plasmids.
J Bacteriol. 1979 Aug; 139(2):597-607.JB

Abstract

Replication-defective mutants of plasmid ColE1 were isolated from a chimeric plasmid formed by ligating a temperature-sensitive replication derivative of pSC101, pHSG1, with a ColE1-Tn3-containing plasmid. The replication-defective ColE1 mutants isolated were all spontaneous deletion mutants that had lost the ColE1 replication origin and regions adjacent to it. The extent of a deletion was determined by analyzing restriction endonuclease-generated deoxyribonucleic acid fragments of the ColE1 plasmid component of the chimeras by both agarose and polyacrylamide gel electrophoresis. None of the chimeras containing the replication-defective ColE1 mutants was able to replicate in the presence of chloramphenicol. The expression of ColE1 incompatibility was either markedly reduced or not detectable in the replication mutants isolated.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

378979

Citation

Hashimoto-Gotoh, T, and J Inselburg. "Isolation and Characterization of Replication-deficient Mutants of ColE1 Plasmids." Journal of Bacteriology, vol. 139, no. 2, 1979, pp. 597-607.
Hashimoto-Gotoh T, Inselburg J. Isolation and characterization of replication-deficient mutants of ColE1 plasmids. J Bacteriol. 1979;139(2):597-607.
Hashimoto-Gotoh, T., & Inselburg, J. (1979). Isolation and characterization of replication-deficient mutants of ColE1 plasmids. Journal of Bacteriology, 139(2), 597-607.
Hashimoto-Gotoh T, Inselburg J. Isolation and Characterization of Replication-deficient Mutants of ColE1 Plasmids. J Bacteriol. 1979;139(2):597-607. PubMed PMID: 378979.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and characterization of replication-deficient mutants of ColE1 plasmids. AU - Hashimoto-Gotoh,T, AU - Inselburg,J, PY - 1979/8/1/pubmed PY - 1979/8/1/medline PY - 1979/8/1/entrez SP - 597 EP - 607 JF - Journal of bacteriology JO - J. Bacteriol. VL - 139 IS - 2 N2 - Replication-defective mutants of plasmid ColE1 were isolated from a chimeric plasmid formed by ligating a temperature-sensitive replication derivative of pSC101, pHSG1, with a ColE1-Tn3-containing plasmid. The replication-defective ColE1 mutants isolated were all spontaneous deletion mutants that had lost the ColE1 replication origin and regions adjacent to it. The extent of a deletion was determined by analyzing restriction endonuclease-generated deoxyribonucleic acid fragments of the ColE1 plasmid component of the chimeras by both agarose and polyacrylamide gel electrophoresis. None of the chimeras containing the replication-defective ColE1 mutants was able to replicate in the presence of chloramphenicol. The expression of ColE1 incompatibility was either markedly reduced or not detectable in the replication mutants isolated. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/378979/Isolation_and_characterization_of_replication_deficient_mutants_of_ColE1_plasmids_ L2 - http://joi.jlc.jst.go.jp/JST.JSTAGE/jsci/139.597?lang=en&from=PubMed DB - PRIME DP - Unbound Medicine ER -