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ColE1 plasmid incompatibility: localization and analysis of mutations affecting incompatibility.
J Bacteriol. 1979 Aug; 139(2):608-19.JB

Abstract

Deletion mutants of plasmid ColE1 that involve the replication origin and adjacent regions of the plasmid have been studied to determine the mechanism by which those mutations affect the expression of plasmid incompatibility. It was observed that (i) a region of ColE1 that is involved in the expression of plasmid incompatibility lies between base pairs -185 and -684; (ii) the integrity of at least part of the region of ColE1 DNA between base pairs -185 and -572 is essential for the expression of ColE1 incompatibility; (iii) the expression of incompatibility is independent of the ability of the ColE1 genome to replicate autonomously; (iv) plasmid incompatibility is affected by plasmid copy number; and (v) ColE1 plasmid-mediated DNA replication of the lambda phage-ColE1 chimera lambda imm434 Oam29 Pam3 ColE1 is inhibited by ColE1-incompatible but not by ColE1-compatible plasmids.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

378980

Citation

Hashimoto-Gotoh, T, and J Inselburg. "ColE1 Plasmid Incompatibility: Localization and Analysis of Mutations Affecting Incompatibility." Journal of Bacteriology, vol. 139, no. 2, 1979, pp. 608-19.
Hashimoto-Gotoh T, Inselburg J. ColE1 plasmid incompatibility: localization and analysis of mutations affecting incompatibility. J Bacteriol. 1979;139(2):608-19.
Hashimoto-Gotoh, T., & Inselburg, J. (1979). ColE1 plasmid incompatibility: localization and analysis of mutations affecting incompatibility. Journal of Bacteriology, 139(2), 608-19.
Hashimoto-Gotoh T, Inselburg J. ColE1 Plasmid Incompatibility: Localization and Analysis of Mutations Affecting Incompatibility. J Bacteriol. 1979;139(2):608-19. PubMed PMID: 378980.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - ColE1 plasmid incompatibility: localization and analysis of mutations affecting incompatibility. AU - Hashimoto-Gotoh,T, AU - Inselburg,J, PY - 1979/8/1/pubmed PY - 1979/8/1/medline PY - 1979/8/1/entrez SP - 608 EP - 19 JF - Journal of bacteriology JO - J Bacteriol VL - 139 IS - 2 N2 - Deletion mutants of plasmid ColE1 that involve the replication origin and adjacent regions of the plasmid have been studied to determine the mechanism by which those mutations affect the expression of plasmid incompatibility. It was observed that (i) a region of ColE1 that is involved in the expression of plasmid incompatibility lies between base pairs -185 and -684; (ii) the integrity of at least part of the region of ColE1 DNA between base pairs -185 and -572 is essential for the expression of ColE1 incompatibility; (iii) the expression of incompatibility is independent of the ability of the ColE1 genome to replicate autonomously; (iv) plasmid incompatibility is affected by plasmid copy number; and (v) ColE1 plasmid-mediated DNA replication of the lambda phage-ColE1 chimera lambda imm434 Oam29 Pam3 ColE1 is inhibited by ColE1-incompatible but not by ColE1-compatible plasmids. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/378980/ColE1_plasmid_incompatibility:_localization_and_analysis_of_mutations_affecting_incompatibility_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=378980 DB - PRIME DP - Unbound Medicine ER -