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[Control of plasmid incompatibility: characteristics of the bireplicon hybrid pAS8 and its deletion mutants].
Genetika. 1979 Jun; 15(6):972-88.G

Abstract

The phenomenon of incompatibility has been investigated using deletion mutants of hybrid bireplicon plasmid pAS8. The hybrid pAS8 displays incompatibility specific for both components of its structure. In contrast to P-specificity of pAS8, functions of ColE1-specificity are not effectively expressed. Expression of ColE1-specificity in pAS8 plasmid and its derivatives is characterized by different directions and this is due to the presence or absence of genes of RP4 replication machinery in the plasmid DNA. Mutant plasmids show different efficiency of P-specificity depending on the extension of deletion in the region of essential genes of the RP4 component. Some of the mutants, in spite of the loss of replication genes, including origin of vegetative replication, are incompatible with the representatives of the Inc P group in both directions of testing. Different character and the level of expression of ColE1- and P-specificity in the pAS8 hybrid and its deletion derivatives are not associated with change in the number of plasmid DNA copies, for all of them are subjects to stringent control of replication. The data suggest the existence of incompatibility functions control mechanism which does not seem to include replication genes. Possible ways of realization of the inc genes functions are discussed.

Authors

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Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

381102

Citation

Sakanian, V A., et al. "[Control of Plasmid Incompatibility: Characteristics of the Bireplicon Hybrid pAS8 and Its Deletion Mutants]." Genetika, vol. 15, no. 6, 1979, pp. 972-88.
Sakanian VA, Krupenko MA, Riabchenko LE, et al. [Control of plasmid incompatibility: characteristics of the bireplicon hybrid pAS8 and its deletion mutants]. Genetika. 1979;15(6):972-88.
Sakanian, V. A., Krupenko, M. A., Riabchenko, L. E., Permogorov, V. I., & Alikhanian, S. I. (1979). [Control of plasmid incompatibility: characteristics of the bireplicon hybrid pAS8 and its deletion mutants]. Genetika, 15(6), 972-88.
Sakanian VA, et al. [Control of Plasmid Incompatibility: Characteristics of the Bireplicon Hybrid pAS8 and Its Deletion Mutants]. Genetika. 1979;15(6):972-88. PubMed PMID: 381102.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Control of plasmid incompatibility: characteristics of the bireplicon hybrid pAS8 and its deletion mutants]. AU - Sakanian,V A, AU - Krupenko,M A, AU - Riabchenko,L E, AU - Permogorov,V I, AU - Alikhanian,S I, PY - 1979/6/1/pubmed PY - 1979/6/1/medline PY - 1979/6/1/entrez SP - 972 EP - 88 JF - Genetika JO - Genetika VL - 15 IS - 6 N2 - The phenomenon of incompatibility has been investigated using deletion mutants of hybrid bireplicon plasmid pAS8. The hybrid pAS8 displays incompatibility specific for both components of its structure. In contrast to P-specificity of pAS8, functions of ColE1-specificity are not effectively expressed. Expression of ColE1-specificity in pAS8 plasmid and its derivatives is characterized by different directions and this is due to the presence or absence of genes of RP4 replication machinery in the plasmid DNA. Mutant plasmids show different efficiency of P-specificity depending on the extension of deletion in the region of essential genes of the RP4 component. Some of the mutants, in spite of the loss of replication genes, including origin of vegetative replication, are incompatible with the representatives of the Inc P group in both directions of testing. Different character and the level of expression of ColE1- and P-specificity in the pAS8 hybrid and its deletion derivatives are not associated with change in the number of plasmid DNA copies, for all of them are subjects to stringent control of replication. The data suggest the existence of incompatibility functions control mechanism which does not seem to include replication genes. Possible ways of realization of the inc genes functions are discussed. SN - 0016-6758 UR - https://www.unboundmedicine.com/medline/citation/381102/[Control_of_plasmid_incompatibility:_characteristics_of_the_bireplicon_hybrid_pAS8_and_its_deletion_mutants]_ DB - PRIME DP - Unbound Medicine ER -