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Evidence for new factors in the coordinate regulation of energy metabolism in Escherichia coli. Effects of hypoxia, chloramphenicol succinate, and 2,4-dinitrophenol on glucose utilization, glycogen synthesis, adenylate energy charge, and hexose phosphates during the first two periods of nitrogen starvation.
J Biol Chem. 1979 Sep 10; 254(17):8295-307.JB

Abstract

We studied the effects of decreased aeration, chloramphenicol succinate, and 2,4-dinitrophenol on the cellular rates of glycogen synthesis and glucose utilization and on the cellular concentrations of adenine nucleotides, glucose 6-phosphate, fructose 1,6-diphosphate, and phosphoenolpyruvate during the first two periods of nitrogen starvation of Escherichia coli W4597(K). A quantitative relationship between the changes in the rates and the accompanying changes in the hexose phosphates is demonstrated. However, the relationship for glycogen synthesis is different in different sets of metabolic conditions. We suggest that this difference reflects a change in the steady state level of a previously unknown effector of ADP-glucose synthetase (glucose 1-phosphate adenylyltransferase, EC 2.7.7.27) the rate-limiting enzyme of bacterial glycogen synthesis. We show that the properties of the hypothetical in vivo effector are consistent with the inhibitory effects of ppGpp (guanosine 3'-diphosphate 5'-diphosphate) and pppGpp (guanosine 3'-diphosphate 5'-triphosphate) on this enzyme in vitro. In addition, tetracycline, an inhibitor of the synthesis of these nucleotides, apparently prevents the change in the quantitative relationship. The relationship between glucose utilization and the hexose phosphates is altered at the transition to Period II of nitrogen starvation. We propose that this change reflects the alteration of the cellular steady state level of an unknown effector of the glucose phosphotransferase system. In contrast to the ATP-hexose phosphate system of shared regulatory effects, the specific effects of the unknown effectors allow the rates of glucose utilization and glycogen synthesis to be altered independently of each other and independently of changes in the rate of glycolysis. This independence allows a greater latitude of response for the individual pathways in more severe metabolic stress or in accommodating the metabolic changes necessary for long term survival.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

381303

Citation

Dietzler, D N., et al. "Evidence for New Factors in the Coordinate Regulation of Energy Metabolism in Escherichia Coli. Effects of Hypoxia, Chloramphenicol Succinate, and 2,4-dinitrophenol On Glucose Utilization, Glycogen Synthesis, Adenylate Energy Charge, and Hexose Phosphates During the First Two Periods of Nitrogen Starvation." The Journal of Biological Chemistry, vol. 254, no. 17, 1979, pp. 8295-307.
Dietzler DN, Leckie MP, Lewis JW, et al. Evidence for new factors in the coordinate regulation of energy metabolism in Escherichia coli. Effects of hypoxia, chloramphenicol succinate, and 2,4-dinitrophenol on glucose utilization, glycogen synthesis, adenylate energy charge, and hexose phosphates during the first two periods of nitrogen starvation. J Biol Chem. 1979;254(17):8295-307.
Dietzler, D. N., Leckie, M. P., Lewis, J. W., Porter, S. E., Taxman, T. L., & Lais, C. J. (1979). Evidence for new factors in the coordinate regulation of energy metabolism in Escherichia coli. Effects of hypoxia, chloramphenicol succinate, and 2,4-dinitrophenol on glucose utilization, glycogen synthesis, adenylate energy charge, and hexose phosphates during the first two periods of nitrogen starvation. The Journal of Biological Chemistry, 254(17), 8295-307.
Dietzler DN, et al. Evidence for New Factors in the Coordinate Regulation of Energy Metabolism in Escherichia Coli. Effects of Hypoxia, Chloramphenicol Succinate, and 2,4-dinitrophenol On Glucose Utilization, Glycogen Synthesis, Adenylate Energy Charge, and Hexose Phosphates During the First Two Periods of Nitrogen Starvation. J Biol Chem. 1979 Sep 10;254(17):8295-307. PubMed PMID: 381303.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence for new factors in the coordinate regulation of energy metabolism in Escherichia coli. Effects of hypoxia, chloramphenicol succinate, and 2,4-dinitrophenol on glucose utilization, glycogen synthesis, adenylate energy charge, and hexose phosphates during the first two periods of nitrogen starvation. AU - Dietzler,D N, AU - Leckie,M P, AU - Lewis,J W, AU - Porter,S E, AU - Taxman,T L, AU - Lais,C J, PY - 1979/9/10/pubmed PY - 1979/9/10/medline PY - 1979/9/10/entrez SP - 8295 EP - 307 JF - The Journal of biological chemistry JO - J Biol Chem VL - 254 IS - 17 N2 - We studied the effects of decreased aeration, chloramphenicol succinate, and 2,4-dinitrophenol on the cellular rates of glycogen synthesis and glucose utilization and on the cellular concentrations of adenine nucleotides, glucose 6-phosphate, fructose 1,6-diphosphate, and phosphoenolpyruvate during the first two periods of nitrogen starvation of Escherichia coli W4597(K). A quantitative relationship between the changes in the rates and the accompanying changes in the hexose phosphates is demonstrated. However, the relationship for glycogen synthesis is different in different sets of metabolic conditions. We suggest that this difference reflects a change in the steady state level of a previously unknown effector of ADP-glucose synthetase (glucose 1-phosphate adenylyltransferase, EC 2.7.7.27) the rate-limiting enzyme of bacterial glycogen synthesis. We show that the properties of the hypothetical in vivo effector are consistent with the inhibitory effects of ppGpp (guanosine 3'-diphosphate 5'-diphosphate) and pppGpp (guanosine 3'-diphosphate 5'-triphosphate) on this enzyme in vitro. In addition, tetracycline, an inhibitor of the synthesis of these nucleotides, apparently prevents the change in the quantitative relationship. The relationship between glucose utilization and the hexose phosphates is altered at the transition to Period II of nitrogen starvation. We propose that this change reflects the alteration of the cellular steady state level of an unknown effector of the glucose phosphotransferase system. In contrast to the ATP-hexose phosphate system of shared regulatory effects, the specific effects of the unknown effectors allow the rates of glucose utilization and glycogen synthesis to be altered independently of each other and independently of changes in the rate of glycolysis. This independence allows a greater latitude of response for the individual pathways in more severe metabolic stress or in accommodating the metabolic changes necessary for long term survival. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/381303/Evidence_for_new_factors_in_the_coordinate_regulation_of_energy_metabolism_in_Escherichia_coli__Effects_of_hypoxia_chloramphenicol_succinate_and_24_dinitrophenol_on_glucose_utilization_glycogen_synthesis_adenylate_energy_charge_and_hexose_phosphates_during_the_first_two_periods_of_nitrogen_starvation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(19)86889-8 DB - PRIME DP - Unbound Medicine ER -