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The effects of an Escherichia coli dnaAts mutation on the replication of the plasmids colE1 pSC101, R100.1 and RTF-TC.
Mol Gen Genet. 1979 Jul 13; 174(2):117-26.MG

Abstract

The rate of replication of the plasmids colE1, pSC101, R100.1 and pAR132 (an RTF-TC derivative of the drug resistance factor R100.1) has been investigated directly by DNA:DNA hybridization. These rates have been compared, in a dnaAts strain, to that of various markers of the host chromosome at permissive and non-permissive temperatures. Chromosome initiation in the dnaAts strain stops rapidly after a shift to the non-permissive temperature, but plasmids R100.1 and pAR132 do not seem to be affected directly and continue replication for some time. The colE1 replication rate undergoes a large increase after the temperature shift, followed by a rapid decrease to a very low level 25 min after the shift. In contrast pSC101 replication stops immediately after the shift. ColE1 is able to replicate in an integratively suppressed dnaAts strain at 42 degrees C whereas pSC101 stops replication immediately under these conditions. We conclude that R100.1 and its derivative RTF-TC can replicate without a functional dnaA product; that colE1, while affected by a shift in temperature in a dnaAts strain, does not directly require dnaA; and that the plasmid pSC101 has an absolute requirement for dnaA. The absolute requirement of pSC101 for dnaA in the integratively suppressed Hfr strain provides a useful system for further investigation of the dnaA function.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

386040

Citation

Frey, J, et al. "The Effects of an Escherichia Coli dnaAts Mutation On the Replication of the Plasmids colE1 pSC101, R100.1 and RTF-TC." Molecular & General Genetics : MGG, vol. 174, no. 2, 1979, pp. 117-26.
Frey J, Chandler M, Caro L. The effects of an Escherichia coli dnaAts mutation on the replication of the plasmids colE1 pSC101, R100.1 and RTF-TC. Mol Gen Genet. 1979;174(2):117-26.
Frey, J., Chandler, M., & Caro, L. (1979). The effects of an Escherichia coli dnaAts mutation on the replication of the plasmids colE1 pSC101, R100.1 and RTF-TC. Molecular & General Genetics : MGG, 174(2), 117-26.
Frey J, Chandler M, Caro L. The Effects of an Escherichia Coli dnaAts Mutation On the Replication of the Plasmids colE1 pSC101, R100.1 and RTF-TC. Mol Gen Genet. 1979 Jul 13;174(2):117-26. PubMed PMID: 386040.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effects of an Escherichia coli dnaAts mutation on the replication of the plasmids colE1 pSC101, R100.1 and RTF-TC. AU - Frey,J, AU - Chandler,M, AU - Caro,L, PY - 1979/7/13/pubmed PY - 1979/7/13/medline PY - 1979/7/13/entrez SP - 117 EP - 26 JF - Molecular & general genetics : MGG JO - Mol Gen Genet VL - 174 IS - 2 N2 - The rate of replication of the plasmids colE1, pSC101, R100.1 and pAR132 (an RTF-TC derivative of the drug resistance factor R100.1) has been investigated directly by DNA:DNA hybridization. These rates have been compared, in a dnaAts strain, to that of various markers of the host chromosome at permissive and non-permissive temperatures. Chromosome initiation in the dnaAts strain stops rapidly after a shift to the non-permissive temperature, but plasmids R100.1 and pAR132 do not seem to be affected directly and continue replication for some time. The colE1 replication rate undergoes a large increase after the temperature shift, followed by a rapid decrease to a very low level 25 min after the shift. In contrast pSC101 replication stops immediately after the shift. ColE1 is able to replicate in an integratively suppressed dnaAts strain at 42 degrees C whereas pSC101 stops replication immediately under these conditions. We conclude that R100.1 and its derivative RTF-TC can replicate without a functional dnaA product; that colE1, while affected by a shift in temperature in a dnaAts strain, does not directly require dnaA; and that the plasmid pSC101 has an absolute requirement for dnaA. The absolute requirement of pSC101 for dnaA in the integratively suppressed Hfr strain provides a useful system for further investigation of the dnaA function. SN - 0026-8925 UR - https://www.unboundmedicine.com/medline/citation/386040/The_effects_of_an_Escherichia_coli_dnaAts_mutation_on_the_replication_of_the_plasmids_colE1_pSC101_R100_1_and_RTF_TC_ L2 - https://doi.org/10.1007/BF00268349 DB - PRIME DP - Unbound Medicine ER -