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Genetic analysis of the inter-relationship between plasmid replication and incompatibility.
Mol Gen Genet. 1979 Jul 13; 174(2):135-47.MG

Abstract

The relationship between replication control and plasmid incompatibility has been investigated using a composite replicon, pPM1, which consists of the pSC101 plasmid ligated to another small multicopy plasmid, RSF1050. Since pPM1 can utilise the replication system of either of the two functionally distinct components, propagation of the composite plasmid can occur in the presence of a mutation of one of its moieties. Such mutants are detected by their inability to rescue the composite plasmid under conditions not permissive for replication of the other moiety. Mutations in incompatibility functions can be detected by the failure of the composite replicon to exclude co-existing plasmids carrying a replication system identical to the one on pPM1. The inability of the composite plasmid to replicate at 42 degrees in a host synthesizing temperature-sensitive DNA polymerase I, which is required by the RSF1050 replication system, was used to isolate pPM1 mutants defective in replication of the pSC101 component. Mutants defective in the incompatibility functions of pSC101 were obtained by selecting derivatives that allow the stable coexistence of a second pSC101 replicon in the same cell. Analysis of these two classes of mutants indicates that plasmids selected for defective pSC101 replication ability nervertheless retain pSC101 incompatibility. In contrast, plasmid mutants that have lost incompatibility functions were found always to be defective in replication ability.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

386041

Citation

Meacock, P A., and S N. Cohen. "Genetic Analysis of the Inter-relationship Between Plasmid Replication and Incompatibility." Molecular & General Genetics : MGG, vol. 174, no. 2, 1979, pp. 135-47.
Meacock PA, Cohen SN. Genetic analysis of the inter-relationship between plasmid replication and incompatibility. Mol Gen Genet. 1979;174(2):135-47.
Meacock, P. A., & Cohen, S. N. (1979). Genetic analysis of the inter-relationship between plasmid replication and incompatibility. Molecular & General Genetics : MGG, 174(2), 135-47.
Meacock PA, Cohen SN. Genetic Analysis of the Inter-relationship Between Plasmid Replication and Incompatibility. Mol Gen Genet. 1979 Jul 13;174(2):135-47. PubMed PMID: 386041.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic analysis of the inter-relationship between plasmid replication and incompatibility. AU - Meacock,P A, AU - Cohen,S N, PY - 1979/7/13/pubmed PY - 1979/7/13/medline PY - 1979/7/13/entrez SP - 135 EP - 47 JF - Molecular & general genetics : MGG JO - Mol Gen Genet VL - 174 IS - 2 N2 - The relationship between replication control and plasmid incompatibility has been investigated using a composite replicon, pPM1, which consists of the pSC101 plasmid ligated to another small multicopy plasmid, RSF1050. Since pPM1 can utilise the replication system of either of the two functionally distinct components, propagation of the composite plasmid can occur in the presence of a mutation of one of its moieties. Such mutants are detected by their inability to rescue the composite plasmid under conditions not permissive for replication of the other moiety. Mutations in incompatibility functions can be detected by the failure of the composite replicon to exclude co-existing plasmids carrying a replication system identical to the one on pPM1. The inability of the composite plasmid to replicate at 42 degrees in a host synthesizing temperature-sensitive DNA polymerase I, which is required by the RSF1050 replication system, was used to isolate pPM1 mutants defective in replication of the pSC101 component. Mutants defective in the incompatibility functions of pSC101 were obtained by selecting derivatives that allow the stable coexistence of a second pSC101 replicon in the same cell. Analysis of these two classes of mutants indicates that plasmids selected for defective pSC101 replication ability nervertheless retain pSC101 incompatibility. In contrast, plasmid mutants that have lost incompatibility functions were found always to be defective in replication ability. SN - 0026-8925 UR - https://www.unboundmedicine.com/medline/citation/386041/Genetic_analysis_of_the_inter_relationship_between_plasmid_replication_and_incompatibility_ L2 - https://doi.org/10.1007/BF00268351 DB - PRIME DP - Unbound Medicine ER -