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Modification of otitis media in chinchillas rechallenged with nontypable Haemophilus influenzae and serological response to outer membrane antigens.
J Infect Dis. 1985 Feb; 151(2):273-9.JI

Abstract

Otitis media was produced in chinchillas by right-sided intrabullar inoculation with nontypable Haemophilus influenzae, and susceptibility to reinfection was investigated. After resolution of initial right-sided infection, animals underwent ipsilateral or contralateral intrabullar rechallenge with the same strain. After ipsilateral rechallenge right ears were completely protected against reinfection; previously uninfected left ears were similarly protected on contralateral rechallenge. Previously infected ears remained fully susceptible to infection with a heterologous strain of nontypable H. influenzae. Using an enzyme-linked immunosorbent assay, we measured the serological response to outer membrane protein and lipopolysaccharide antigens during initial infection. A greater than or equal to 10-fold rise in titer of antibody to homologous outer membrane proteins was observed in all 11 animals tested. Most animals exhibited a minimal serological response to lipopolysaccharide. Thus experimental otitis media due to nontypable H. influenzae induces strain-specific protective immunity and a concomitant serological response to outer membrane proteins.

Authors

Karasic RB
No affiliation info available
Trumpp CE
No affiliation info available
Gnehm HE
No affiliation info available
Rice PA
No affiliation info available
Pelton SI
No affiliation info available

MeSH

AnimalsAntibodies, BacterialAntigens, BacterialBacterial Outer Membrane ProteinsChinchillaCross ReactionsEnzyme-Linked Immunosorbent AssayHaemophilus InfectionsHaemophilus influenzaeLipopolysaccharidesOtitis MediaOtitis Media with Effusion

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3871464

Citation

Karasic, R B., et al. "Modification of Otitis Media in Chinchillas Rechallenged With Nontypable Haemophilus Influenzae and Serological Response to Outer Membrane Antigens." The Journal of Infectious Diseases, vol. 151, no. 2, 1985, pp. 273-9.
Karasic RB, Trumpp CE, Gnehm HE, et al. Modification of otitis media in chinchillas rechallenged with nontypable Haemophilus influenzae and serological response to outer membrane antigens. J Infect Dis. 1985;151(2):273-9.
Karasic, R. B., Trumpp, C. E., Gnehm, H. E., Rice, P. A., & Pelton, S. I. (1985). Modification of otitis media in chinchillas rechallenged with nontypable Haemophilus influenzae and serological response to outer membrane antigens. The Journal of Infectious Diseases, 151(2), 273-9.
Karasic RB, et al. Modification of Otitis Media in Chinchillas Rechallenged With Nontypable Haemophilus Influenzae and Serological Response to Outer Membrane Antigens. J Infect Dis. 1985;151(2):273-9. PubMed PMID: 3871464.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modification of otitis media in chinchillas rechallenged with nontypable Haemophilus influenzae and serological response to outer membrane antigens. AU - Karasic,R B, AU - Trumpp,C E, AU - Gnehm,H E, AU - Rice,P A, AU - Pelton,S I, PY - 1985/2/1/pubmed PY - 1985/2/1/medline PY - 1985/2/1/entrez SP - 273 EP - 9 JF - The Journal of infectious diseases JO - J Infect Dis VL - 151 IS - 2 N2 - Otitis media was produced in chinchillas by right-sided intrabullar inoculation with nontypable Haemophilus influenzae, and susceptibility to reinfection was investigated. After resolution of initial right-sided infection, animals underwent ipsilateral or contralateral intrabullar rechallenge with the same strain. After ipsilateral rechallenge right ears were completely protected against reinfection; previously uninfected left ears were similarly protected on contralateral rechallenge. Previously infected ears remained fully susceptible to infection with a heterologous strain of nontypable H. influenzae. Using an enzyme-linked immunosorbent assay, we measured the serological response to outer membrane protein and lipopolysaccharide antigens during initial infection. A greater than or equal to 10-fold rise in titer of antibody to homologous outer membrane proteins was observed in all 11 animals tested. Most animals exhibited a minimal serological response to lipopolysaccharide. Thus experimental otitis media due to nontypable H. influenzae induces strain-specific protective immunity and a concomitant serological response to outer membrane proteins. SN - 0022-1899 UR - https://www.unboundmedicine.com/medline/citation/3871464/Modification_of_otitis_media_in_chinchillas_rechallenged_with_nontypable_Haemophilus_influenzae_and_serological_response_to_outer_membrane_antigens_ DB - PRIME DP - Unbound Medicine ER -