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Androgen cytosol binding during compensatory overload-induced skeletal muscle hypertrophy.
Can J Biochem Cell Biol. 1985 May; 63(5):348-54.CJ

Abstract

This study was undertaken to evaluate whether the increased androgen cytosol binding is an early or later event in the sequence of skeletal muscle hypertrophy induced by surgical overload. Following removal of the synergistic gastrocnemius and soleus muscles, plantaris muscle weights of overloaded hypophysectomized made rats were heavier than those in the control by 29% at 2 days, 41% at 7 days, 38% at 14 days, and 47% at 35 days. Androgen cytosol receptor binding capacities (femtomoles per milligram protein), determined using a synthetic androgen, [3H]methyltrienolone (R1881), were higher than observed in muscles of controls at all points of muscle enlargement. At high concentrations of labeled ligand, Scatchard analyses became nonlinear and were resolved using a two-component binding model. Receptor capacity of the higher affinity "androgenic component" for methyltrienolone binding in plantaris muscles was lower at 2 days but 60-80% higher at 7, 14, and 35 days in the hypertrophied group than in the control group. The lower affinity "glucocorticoid component" was higher in the overloaded group at all points following surgery. Several glucocorticoids and estradiol-17 beta competed equally with androgens for methyltrienolone binding. However, when cytosols were incubated with triamcinolone acetonide to block methyltrienolone binding to glucocorticoid receptors, the androgenic component was highly specific for androgens. These results show that total [3H]methyltrienolone cytosol concentrations increased in parallel with the muscle hypertrophy, yet the individual components of methyltrienolone binding attained greater concentrations in overloaded muscles by an apparently different sequence of events.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3874677

Citation

Hickson, R C., et al. "Androgen Cytosol Binding During Compensatory Overload-induced Skeletal Muscle Hypertrophy." Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire, vol. 63, no. 5, 1985, pp. 348-54.
Hickson RC, Kurowski TT, Galassi TM, et al. Androgen cytosol binding during compensatory overload-induced skeletal muscle hypertrophy. Can J Biochem Cell Biol. 1985;63(5):348-54.
Hickson, R. C., Kurowski, T. T., Galassi, T. M., Daniels, D. G., & Chatterton, R. J. (1985). Androgen cytosol binding during compensatory overload-induced skeletal muscle hypertrophy. Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire, 63(5), 348-54.
Hickson RC, et al. Androgen Cytosol Binding During Compensatory Overload-induced Skeletal Muscle Hypertrophy. Can J Biochem Cell Biol. 1985;63(5):348-54. PubMed PMID: 3874677.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Androgen cytosol binding during compensatory overload-induced skeletal muscle hypertrophy. AU - Hickson,R C, AU - Kurowski,T T, AU - Galassi,T M, AU - Daniels,D G, AU - Chatterton,R J,Jr PY - 1985/5/1/pubmed PY - 2001/3/28/medline PY - 1985/5/1/entrez SP - 348 EP - 54 JF - Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire JO - Can J Biochem Cell Biol VL - 63 IS - 5 N2 - This study was undertaken to evaluate whether the increased androgen cytosol binding is an early or later event in the sequence of skeletal muscle hypertrophy induced by surgical overload. Following removal of the synergistic gastrocnemius and soleus muscles, plantaris muscle weights of overloaded hypophysectomized made rats were heavier than those in the control by 29% at 2 days, 41% at 7 days, 38% at 14 days, and 47% at 35 days. Androgen cytosol receptor binding capacities (femtomoles per milligram protein), determined using a synthetic androgen, [3H]methyltrienolone (R1881), were higher than observed in muscles of controls at all points of muscle enlargement. At high concentrations of labeled ligand, Scatchard analyses became nonlinear and were resolved using a two-component binding model. Receptor capacity of the higher affinity "androgenic component" for methyltrienolone binding in plantaris muscles was lower at 2 days but 60-80% higher at 7, 14, and 35 days in the hypertrophied group than in the control group. The lower affinity "glucocorticoid component" was higher in the overloaded group at all points following surgery. Several glucocorticoids and estradiol-17 beta competed equally with androgens for methyltrienolone binding. However, when cytosols were incubated with triamcinolone acetonide to block methyltrienolone binding to glucocorticoid receptors, the androgenic component was highly specific for androgens. These results show that total [3H]methyltrienolone cytosol concentrations increased in parallel with the muscle hypertrophy, yet the individual components of methyltrienolone binding attained greater concentrations in overloaded muscles by an apparently different sequence of events. SN - 0714-7511 UR - https://www.unboundmedicine.com/medline/citation/3874677/Androgen_cytosol_binding_during_compensatory_overload_induced_skeletal_muscle_hypertrophy_ DB - PRIME DP - Unbound Medicine ER -