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Similar action of platelet-derived growth factor and epidermal growth factor in the prereplicative phase of human fibroblasts suggests a common intracellular pathway.
J Cell Physiol. 1985 Jul; 124(1):43-8.JC

Abstract

We have investigated the effects of platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) in the prereplicative phase of human foreskin fibroblasts cultured under defined conditions in serum-free MCDB 105 medium. Specific antisera against PDGF and EGF were used to inhibit the stimulation after certain incubation times. It was found that PDGF or EGF had to be present during the major part of the G0/G1 phase (greater than 8 h) in order to cause any appreciable commitment to DNA synthesis; half maximal stimulations were obtained after 9 h and 11 h of incubations with PDGF and EGF, respectively. When tested during a suboptimal period of time (6 h), neither an increase in concentration of PDGF or EGF, nor the addition of both growth factors simultaneously caused any appreciable stimulation of DNA synthesis. However, a suboptimal pulse of PDGF, followed by a suboptimal pulse of EGF, or vice versa, led to commitment to DNA synthesis. This finding indicates that PDGF and EGF, at least in part, induce similar intracellular events that transmit the mitogenic signal.

Authors

Westermark B
No affiliation info available
Heldin CH
No affiliation info available

MeSH

Cell DivisionCell LineDNA ReplicationDose-Response Relationship, DrugDrug SynergismEpidermal Growth FactorFibroblastsHumansInterphaseMalePlatelet-Derived Growth FactorThymidineTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3876339

Citation

Westermark, B, and C H. Heldin. "Similar Action of Platelet-derived Growth Factor and Epidermal Growth Factor in the Prereplicative Phase of Human Fibroblasts Suggests a Common Intracellular Pathway." Journal of Cellular Physiology, vol. 124, no. 1, 1985, pp. 43-8.
Westermark B, Heldin CH. Similar action of platelet-derived growth factor and epidermal growth factor in the prereplicative phase of human fibroblasts suggests a common intracellular pathway. J Cell Physiol. 1985;124(1):43-8.
Westermark, B., & Heldin, C. H. (1985). Similar action of platelet-derived growth factor and epidermal growth factor in the prereplicative phase of human fibroblasts suggests a common intracellular pathway. Journal of Cellular Physiology, 124(1), 43-8.
Westermark B, Heldin CH. Similar Action of Platelet-derived Growth Factor and Epidermal Growth Factor in the Prereplicative Phase of Human Fibroblasts Suggests a Common Intracellular Pathway. J Cell Physiol. 1985;124(1):43-8. PubMed PMID: 3876339.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Similar action of platelet-derived growth factor and epidermal growth factor in the prereplicative phase of human fibroblasts suggests a common intracellular pathway. AU - Westermark,B, AU - Heldin,C H, PY - 1985/7/1/pubmed PY - 1985/7/1/medline PY - 1985/7/1/entrez SP - 43 EP - 8 JF - Journal of cellular physiology JO - J Cell Physiol VL - 124 IS - 1 N2 - We have investigated the effects of platelet-derived growth factor (PDGF) and epidermal growth factor (EGF) in the prereplicative phase of human foreskin fibroblasts cultured under defined conditions in serum-free MCDB 105 medium. Specific antisera against PDGF and EGF were used to inhibit the stimulation after certain incubation times. It was found that PDGF or EGF had to be present during the major part of the G0/G1 phase (greater than 8 h) in order to cause any appreciable commitment to DNA synthesis; half maximal stimulations were obtained after 9 h and 11 h of incubations with PDGF and EGF, respectively. When tested during a suboptimal period of time (6 h), neither an increase in concentration of PDGF or EGF, nor the addition of both growth factors simultaneously caused any appreciable stimulation of DNA synthesis. However, a suboptimal pulse of PDGF, followed by a suboptimal pulse of EGF, or vice versa, led to commitment to DNA synthesis. This finding indicates that PDGF and EGF, at least in part, induce similar intracellular events that transmit the mitogenic signal. SN - 0021-9541 UR - https://www.unboundmedicine.com/medline/citation/3876339/Similar_action_of_platelet_derived_growth_factor_and_epidermal_growth_factor_in_the_prereplicative_phase_of_human_fibroblasts_suggests_a_common_intracellular_pathway_ DB - PRIME DP - Unbound Medicine ER -