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DNA content analysis of peripheral blood B-lymphocytes in plasma cell malignancies.
Basic Appl Histochem. 1985; 29(3):275-82.BA

Abstract

A double fluorescence assay has been employed for the detection of cell surface and/or cytoplasmic immunoglobulins (Ig) and the measurement of nuclear DNA content in the same cell. Following staining for Ig by means of FITC conjugated antibodies directed against heavy or light chains, cell suspensions or cytospin preparations were ethanol fixed and stained with a propidium iodide-RNAse solution. In this way, the cytometric DNA content of circulating B-lymphocytes was analyzed in three patients suffering from plasma cell malignancies with an excess of peripheral blood B-lymphocytes and evidence of aneuploid bone marrow plasma cells. Aneuploid circulating B-lymphocytes with the same DNA stem-line as bone marrow plasma cells were found in two patients with advanced disease but not in the only one we studied at presentation. Aneuploid lymphocytes had surface immunoglobulins bearing the same light chain as the M-protein. In addition, a significant percentage (23%) of cells lacking either surface or cytoplasmic immunoglobulins proved to be aneuploid in plasma cell leukemia. Nuclear DNA measurement combined with surface or cytoplasmic marker analysis appears to be a reliable method for studying neoplastic lymphoid precursor cells in plasma cell malignancies.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3877500

Citation

Montecucco, C, et al. "DNA Content Analysis of Peripheral Blood B-lymphocytes in Plasma Cell Malignancies." Basic and Applied Histochemistry, vol. 29, no. 3, 1985, pp. 275-82.
Montecucco C, Riccardi A, Mazzini G, et al. DNA content analysis of peripheral blood B-lymphocytes in plasma cell malignancies. Basic Appl Histochem. 1985;29(3):275-82.
Montecucco, C., Riccardi, A., Mazzini, G., Danova, M., Ucci, G., & Giordano, P. A. (1985). DNA content analysis of peripheral blood B-lymphocytes in plasma cell malignancies. Basic and Applied Histochemistry, 29(3), 275-82.
Montecucco C, et al. DNA Content Analysis of Peripheral Blood B-lymphocytes in Plasma Cell Malignancies. Basic Appl Histochem. 1985;29(3):275-82. PubMed PMID: 3877500.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA content analysis of peripheral blood B-lymphocytes in plasma cell malignancies. AU - Montecucco,C, AU - Riccardi,A, AU - Mazzini,G, AU - Danova,M, AU - Ucci,G, AU - Giordano,P A, PY - 1985/1/1/pubmed PY - 1985/1/1/medline PY - 1985/1/1/entrez SP - 275 EP - 82 JF - Basic and applied histochemistry JO - Basic Appl Histochem VL - 29 IS - 3 N2 - A double fluorescence assay has been employed for the detection of cell surface and/or cytoplasmic immunoglobulins (Ig) and the measurement of nuclear DNA content in the same cell. Following staining for Ig by means of FITC conjugated antibodies directed against heavy or light chains, cell suspensions or cytospin preparations were ethanol fixed and stained with a propidium iodide-RNAse solution. In this way, the cytometric DNA content of circulating B-lymphocytes was analyzed in three patients suffering from plasma cell malignancies with an excess of peripheral blood B-lymphocytes and evidence of aneuploid bone marrow plasma cells. Aneuploid circulating B-lymphocytes with the same DNA stem-line as bone marrow plasma cells were found in two patients with advanced disease but not in the only one we studied at presentation. Aneuploid lymphocytes had surface immunoglobulins bearing the same light chain as the M-protein. In addition, a significant percentage (23%) of cells lacking either surface or cytoplasmic immunoglobulins proved to be aneuploid in plasma cell leukemia. Nuclear DNA measurement combined with surface or cytoplasmic marker analysis appears to be a reliable method for studying neoplastic lymphoid precursor cells in plasma cell malignancies. SN - 0391-7258 UR - https://www.unboundmedicine.com/medline/citation/3877500/DNA_content_analysis_of_peripheral_blood_B_lymphocytes_in_plasma_cell_malignancies_ DB - PRIME DP - Unbound Medicine ER -