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Evidence for absence of toxicity of T101 immunotoxin on human hematopoietic progenitor cells prior to bone marrow transplantation.
Cancer Res. 1985 Jan; 45(1):438-41.CR

Abstract

T101-ricin A-chain immunotoxin is a hybrid molecule made up of the T101 monoclonal antibody bound to the A-chain of ricin. It specifically destroys cells expressing the cell surface T65 antigen. We have designed a preclinical study to evaluate its possible use for the in vitro treatment of T-cell hematological cancers prior to autologous bone marrow transplantation. The data presented here show that conditions previously defined to produce high tumor cell killing, i.e., a 20-hr incubation at 37 degrees in the presence of T101-ricin A-chain immunotoxin up to 10(-7) M in a 10 mM ammonium chloride solution, do not affect the in vitro proliferative capacity of human hematopoietic stem cells studied by means of semisolid medium cultures (granulocyte-macrophage progenitors, burst-forming units-erythrocyte) and continuous liquid cultures (pre-granulocyte-macrophage progenitors). Therefore, autologous bone marrow transplantation with T101-ricin A-chain immunotoxin-treated graft should be feasible.

Authors

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Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3880666

Citation

Douay, L, et al. "Evidence for Absence of Toxicity of T101 Immunotoxin On Human Hematopoietic Progenitor Cells Prior to Bone Marrow Transplantation." Cancer Research, vol. 45, no. 1, 1985, pp. 438-41.
Douay L, Gorin NC, Lopez M, et al. Evidence for absence of toxicity of T101 immunotoxin on human hematopoietic progenitor cells prior to bone marrow transplantation. Cancer Res. 1985;45(1):438-41.
Douay, L., Gorin, N. C., Lopez, M., Casellas, P., Liance, M. C., Jansen, F. K., Voisin, G. A., Baillou, C., Laporte, J. P., & Najman, A. (1985). Evidence for absence of toxicity of T101 immunotoxin on human hematopoietic progenitor cells prior to bone marrow transplantation. Cancer Research, 45(1), 438-41.
Douay L, et al. Evidence for Absence of Toxicity of T101 Immunotoxin On Human Hematopoietic Progenitor Cells Prior to Bone Marrow Transplantation. Cancer Res. 1985;45(1):438-41. PubMed PMID: 3880666.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence for absence of toxicity of T101 immunotoxin on human hematopoietic progenitor cells prior to bone marrow transplantation. A1 - Douay,L, AU - Gorin,N C, AU - Lopez,M, AU - Casellas,P, AU - Liance,M C, AU - Jansen,F K, AU - Voisin,G A, AU - Baillou,C, AU - Laporte,J P, AU - Najman,A, PY - 1985/1/1/pubmed PY - 1985/1/1/medline PY - 1985/1/1/entrez SP - 438 EP - 41 JF - Cancer research JO - Cancer Res VL - 45 IS - 1 N2 - T101-ricin A-chain immunotoxin is a hybrid molecule made up of the T101 monoclonal antibody bound to the A-chain of ricin. It specifically destroys cells expressing the cell surface T65 antigen. We have designed a preclinical study to evaluate its possible use for the in vitro treatment of T-cell hematological cancers prior to autologous bone marrow transplantation. The data presented here show that conditions previously defined to produce high tumor cell killing, i.e., a 20-hr incubation at 37 degrees in the presence of T101-ricin A-chain immunotoxin up to 10(-7) M in a 10 mM ammonium chloride solution, do not affect the in vitro proliferative capacity of human hematopoietic stem cells studied by means of semisolid medium cultures (granulocyte-macrophage progenitors, burst-forming units-erythrocyte) and continuous liquid cultures (pre-granulocyte-macrophage progenitors). Therefore, autologous bone marrow transplantation with T101-ricin A-chain immunotoxin-treated graft should be feasible. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/3880666/Evidence_for_absence_of_toxicity_of_T101_immunotoxin_on_human_hematopoietic_progenitor_cells_prior_to_bone_marrow_transplantation_ L2 - http://cancerres.aacrjournals.org/cgi/pmidlookup?view=long&pmid=3880666 DB - PRIME DP - Unbound Medicine ER -