Tags

Type your tag names separated by a space and hit enter

The diagnosis of vitamin B12 deficiency in sheep: comparison of serum vitamin B12 levels measured by a microbiological and a radioisotope dilution technique.
Ann Rech Vet. 1985; 16(1):51-6.AR

Abstract

Serum vitamin B12 from 6 sheep fed a diet containing 0.06 mg cobalt per kg dry matter (DM) was analysed by a radioisotope dilution (RID) technique. The results were compared with values obtained by a microbiological method (Lactobacillus leichmannii - ATCC 7830) specific for "true" vitamin B12. Serum vitamin B12 concentrations were four to six times greater during the 16 weeks experimental period with the RID method as compared to the microbiological technique. During the course of the experiment, values obtained by the RID method remained superior to the threshold value (200 pg B 12/ml) of the microbiological method. This investigation indicates that the RID method is not suitable for the rapid diagnosis of vitamin B12 deficiency in subclinically cobalt-deficient sheep.

Authors

Mohammed R
No affiliation info available
Lamand M
No affiliation info available

MeSH

AnimalsBiological AssayLactobacillusRadioisotope Dilution TechniqueSheepSheep DiseasesVitamin B 12Vitamin B 12 Deficiency

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

3893301

Citation

Mohammed, R, and M Lamand. "The Diagnosis of Vitamin B12 Deficiency in Sheep: Comparison of Serum Vitamin B12 Levels Measured By a Microbiological and a Radioisotope Dilution Technique." Annales De Recherches Veterinaires. Annals of Veterinary Research, vol. 16, no. 1, 1985, pp. 51-6.
Mohammed R, Lamand M. The diagnosis of vitamin B12 deficiency in sheep: comparison of serum vitamin B12 levels measured by a microbiological and a radioisotope dilution technique. Ann Rech Vet. 1985;16(1):51-6.
Mohammed, R., & Lamand, M. (1985). The diagnosis of vitamin B12 deficiency in sheep: comparison of serum vitamin B12 levels measured by a microbiological and a radioisotope dilution technique. Annales De Recherches Veterinaires. Annals of Veterinary Research, 16(1), 51-6.
Mohammed R, Lamand M. The Diagnosis of Vitamin B12 Deficiency in Sheep: Comparison of Serum Vitamin B12 Levels Measured By a Microbiological and a Radioisotope Dilution Technique. Ann Rech Vet. 1985;16(1):51-6. PubMed PMID: 3893301.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The diagnosis of vitamin B12 deficiency in sheep: comparison of serum vitamin B12 levels measured by a microbiological and a radioisotope dilution technique. AU - Mohammed,R, AU - Lamand,M, PY - 1985/1/1/pubmed PY - 1985/1/1/medline PY - 1985/1/1/entrez SP - 51 EP - 6 JF - Annales de recherches veterinaires. Annals of veterinary research JO - Ann Rech Vet VL - 16 IS - 1 N2 - Serum vitamin B12 from 6 sheep fed a diet containing 0.06 mg cobalt per kg dry matter (DM) was analysed by a radioisotope dilution (RID) technique. The results were compared with values obtained by a microbiological method (Lactobacillus leichmannii - ATCC 7830) specific for "true" vitamin B12. Serum vitamin B12 concentrations were four to six times greater during the 16 weeks experimental period with the RID method as compared to the microbiological technique. During the course of the experiment, values obtained by the RID method remained superior to the threshold value (200 pg B 12/ml) of the microbiological method. This investigation indicates that the RID method is not suitable for the rapid diagnosis of vitamin B12 deficiency in subclinically cobalt-deficient sheep. SN - 0003-4193 UR - https://www.unboundmedicine.com/medline/citation/3893301/The_diagnosis_of_vitamin_B12_deficiency_in_sheep:_comparison_of_serum_vitamin_B12_levels_measured_by_a_microbiological_and_a_radioisotope_dilution_technique_ DB - PRIME DP - Unbound Medicine ER -