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Antibodies to gliadin detected by immunofluorescence and a micro-ELISA method: markers of active childhood and adult coeliac disease.
Gut. 1985 Jul; 26(7):667-71.Gut

Abstract

Antibodies to gliadin have been detected by immunofluorescence (IFL-AGA) and a micro-ELISA method (ELISA-AGA) in 45 out of 47 (96%) sera from patients with active childhood and adult coeliac disease. The two methods were more sensitive than R1-reticulin antibodies (R1-ARA) which were found only in 28 of the same patients (60%). R1-ARA were always negative in the 26 sera from patients with childhood coeliac disease and adult coeliac disease after gluten free diet, while IFL- and ELISA-AGA were respectively found in three (12%) and in four (15%) out of these patients. Moreover, while R1-ARA and IFL-AGA were strictly confined to coeliac disease. ELISA-AGA were occasionally found in patients with control diseases. These 'false positive' antibodies were all of IgG class and had low titres. In our experience IFL- and ELISA-AGA of IgA class were strictly confined to active childhood coeliac disease and adult coeliac disease. The detection of AGA is useful in monitoring the diet and in the follow up of coeliac disease. IFL- and ELISA-AGA, then, are to be preferred to R1-ARA for the screening of coeliac patients.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

3894169

Citation

Volta, U, et al. "Antibodies to Gliadin Detected By Immunofluorescence and a micro-ELISA Method: Markers of Active Childhood and Adult Coeliac Disease." Gut, vol. 26, no. 7, 1985, pp. 667-71.
Volta U, Lenzi M, Lazzari R, et al. Antibodies to gliadin detected by immunofluorescence and a micro-ELISA method: markers of active childhood and adult coeliac disease. Gut. 1985;26(7):667-71.
Volta, U., Lenzi, M., Lazzari, R., Cassani, F., Collina, A., Bianchi, F. B., & Pisi, E. (1985). Antibodies to gliadin detected by immunofluorescence and a micro-ELISA method: markers of active childhood and adult coeliac disease. Gut, 26(7), 667-71.
Volta U, et al. Antibodies to Gliadin Detected By Immunofluorescence and a micro-ELISA Method: Markers of Active Childhood and Adult Coeliac Disease. Gut. 1985;26(7):667-71. PubMed PMID: 3894169.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibodies to gliadin detected by immunofluorescence and a micro-ELISA method: markers of active childhood and adult coeliac disease. AU - Volta,U, AU - Lenzi,M, AU - Lazzari,R, AU - Cassani,F, AU - Collina,A, AU - Bianchi,F B, AU - Pisi,E, PY - 1985/7/1/pubmed PY - 1985/7/1/medline PY - 1985/7/1/entrez SP - 667 EP - 71 JF - Gut JO - Gut VL - 26 IS - 7 N2 - Antibodies to gliadin have been detected by immunofluorescence (IFL-AGA) and a micro-ELISA method (ELISA-AGA) in 45 out of 47 (96%) sera from patients with active childhood and adult coeliac disease. The two methods were more sensitive than R1-reticulin antibodies (R1-ARA) which were found only in 28 of the same patients (60%). R1-ARA were always negative in the 26 sera from patients with childhood coeliac disease and adult coeliac disease after gluten free diet, while IFL- and ELISA-AGA were respectively found in three (12%) and in four (15%) out of these patients. Moreover, while R1-ARA and IFL-AGA were strictly confined to coeliac disease. ELISA-AGA were occasionally found in patients with control diseases. These 'false positive' antibodies were all of IgG class and had low titres. In our experience IFL- and ELISA-AGA of IgA class were strictly confined to active childhood coeliac disease and adult coeliac disease. The detection of AGA is useful in monitoring the diet and in the follow up of coeliac disease. IFL- and ELISA-AGA, then, are to be preferred to R1-ARA for the screening of coeliac patients. SN - 0017-5749 UR - https://www.unboundmedicine.com/medline/citation/3894169/Antibodies_to_gliadin_detected_by_immunofluorescence_and_a_micro_ELISA_method:_markers_of_active_childhood_and_adult_coeliac_disease_ L2 - https://gut.bmj.com/lookup/pmidlookup?view=long&pmid=3894169 DB - PRIME DP - Unbound Medicine ER -