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[Nature of the compatible inheritance of hybrid plasmid pAS8 and its deletion mutants with replicon ColE1].
Genetika 1979; 15(11):1971-8G

Abstract

Hybrid plasmid pAS8, that consists of RP4 and ColE1 replicons, is incompatible with RP4 but co-exists with ColE1 replicon. The deletion mutants of pAS8, that replicates under the control of ColE1 replicon only, are incompatible with ColE1 derivatives, although the copy number of pAS8 and its deletion mutants in the cell is the same (1-3 per the chromosome). Incompatibility effect of plasmids is expressed in a sharp decrease in transformant's yield during selection of incoming and resident plasmids markers. In this case incompatibility is a very fast process, that leads to the elimination of resident or incoming plasmid just before plating on the selective medium. On the base of negative control theory, the repressors yield, synthesized in the presence of 1-3 copies of the deletion mutant is enough for the expression of ColE1-specific incompatibility. This ColE1 incompatibility is probably connected with the functional activity of ColE1 replicon. When ColE1 factor replicates under the control of RP4 replicon the expression of ColE1-specific incompatibility does not occur. Possibly, the incompatibility effect takes place when pAS8 deletion mutants cause the synthesis of ColE1-specific repressor. Also, the replicons of ColE1 may competein the membrane attachment site.

Authors

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Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

389735

Citation

Stepanov, A I., et al. "[Nature of the Compatible Inheritance of Hybrid Plasmid pAS8 and Its Deletion Mutants With Replicon ColE1]." Genetika, vol. 15, no. 11, 1979, pp. 1971-8.
Stepanov AI, Urlapova SV, Zimina MS. [Nature of the compatible inheritance of hybrid plasmid pAS8 and its deletion mutants with replicon ColE1]. Genetika. 1979;15(11):1971-8.
Stepanov, A. I., Urlapova, S. V., & Zimina, M. S. (1979). [Nature of the compatible inheritance of hybrid plasmid pAS8 and its deletion mutants with replicon ColE1]. Genetika, 15(11), pp. 1971-8.
Stepanov AI, Urlapova SV, Zimina MS. [Nature of the Compatible Inheritance of Hybrid Plasmid pAS8 and Its Deletion Mutants With Replicon ColE1]. Genetika. 1979;15(11):1971-8. PubMed PMID: 389735.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Nature of the compatible inheritance of hybrid plasmid pAS8 and its deletion mutants with replicon ColE1]. AU - Stepanov,A I, AU - Urlapova,S V, AU - Zimina,M S, PY - 1979/11/1/pubmed PY - 1979/11/1/medline PY - 1979/11/1/entrez SP - 1971 EP - 8 JF - Genetika JO - Genetika VL - 15 IS - 11 N2 - Hybrid plasmid pAS8, that consists of RP4 and ColE1 replicons, is incompatible with RP4 but co-exists with ColE1 replicon. The deletion mutants of pAS8, that replicates under the control of ColE1 replicon only, are incompatible with ColE1 derivatives, although the copy number of pAS8 and its deletion mutants in the cell is the same (1-3 per the chromosome). Incompatibility effect of plasmids is expressed in a sharp decrease in transformant's yield during selection of incoming and resident plasmids markers. In this case incompatibility is a very fast process, that leads to the elimination of resident or incoming plasmid just before plating on the selective medium. On the base of negative control theory, the repressors yield, synthesized in the presence of 1-3 copies of the deletion mutant is enough for the expression of ColE1-specific incompatibility. This ColE1 incompatibility is probably connected with the functional activity of ColE1 replicon. When ColE1 factor replicates under the control of RP4 replicon the expression of ColE1-specific incompatibility does not occur. Possibly, the incompatibility effect takes place when pAS8 deletion mutants cause the synthesis of ColE1-specific repressor. Also, the replicons of ColE1 may competein the membrane attachment site. SN - 0016-6758 UR - https://www.unboundmedicine.com/medline/citation/389735/[Nature_of_the_compatible_inheritance_of_hybrid_plasmid_pAS8_and_its_deletion_mutants_with_replicon_ColE1]_ DB - PRIME DP - Unbound Medicine ER -