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Yeast ribosomal protein L25 binds to an evolutionary conserved site on yeast 26S and E. coli 23S rRNA.
EMBO J. 1985 Aug; 4(8):2101-7.EJ

Abstract

The binding site of the yeast 60S ribosomal subunit protein L25 on 26S rRNA was determined by RNase protection experiments. The fragments protected by L25 originate from a distinct substructure within domain IV of the rRNA, encompassing nucleotides 1465-1632 and 1811-1861. The protected fragments are able to rebind to L25 showing that they constitute the complete protein binding site. This binding site is remarkably conserved in all 23/26/28S rRNAs sequenced to date including Escherichia coli 23S rRNA. In fact heterologous complexes between L25 and E. coli 23S rRNA could be formed and RNase protection studies on these complexes demonstrated that L25 indeed recognizes the conserved structure. Strikingly the L25 binding site on 23S rRNA is virtually identical to the previously identified binding site of E. coli ribosomal protein EL23. Therefore EL23 is likely to be the prokaryotic counterpart of L25 in spite of the limited homology displayed by the amino acid sequences of the two proteins.

Authors

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Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3905389

Citation

el-Baradi, T T., et al. "Yeast Ribosomal Protein L25 Binds to an Evolutionary Conserved Site On Yeast 26S and E. Coli 23S RRNA." The EMBO Journal, vol. 4, no. 8, 1985, pp. 2101-7.
el-Baradi TT, Raué HA, de Regt VC, et al. Yeast ribosomal protein L25 binds to an evolutionary conserved site on yeast 26S and E. coli 23S rRNA. EMBO J. 1985;4(8):2101-7.
el-Baradi, T. T., Raué, H. A., de Regt, V. C., Verbree, E. C., & Planta, R. J. (1985). Yeast ribosomal protein L25 binds to an evolutionary conserved site on yeast 26S and E. coli 23S rRNA. The EMBO Journal, 4(8), 2101-7.
el-Baradi TT, et al. Yeast Ribosomal Protein L25 Binds to an Evolutionary Conserved Site On Yeast 26S and E. Coli 23S RRNA. EMBO J. 1985;4(8):2101-7. PubMed PMID: 3905389.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Yeast ribosomal protein L25 binds to an evolutionary conserved site on yeast 26S and E. coli 23S rRNA. AU - el-Baradi,T T, AU - Raué,H A, AU - de Regt,V C, AU - Verbree,E C, AU - Planta,R J, PY - 1985/8/1/pubmed PY - 1985/8/1/medline PY - 1985/8/1/entrez SP - 2101 EP - 7 JF - The EMBO journal JO - EMBO J VL - 4 IS - 8 N2 - The binding site of the yeast 60S ribosomal subunit protein L25 on 26S rRNA was determined by RNase protection experiments. The fragments protected by L25 originate from a distinct substructure within domain IV of the rRNA, encompassing nucleotides 1465-1632 and 1811-1861. The protected fragments are able to rebind to L25 showing that they constitute the complete protein binding site. This binding site is remarkably conserved in all 23/26/28S rRNAs sequenced to date including Escherichia coli 23S rRNA. In fact heterologous complexes between L25 and E. coli 23S rRNA could be formed and RNase protection studies on these complexes demonstrated that L25 indeed recognizes the conserved structure. Strikingly the L25 binding site on 23S rRNA is virtually identical to the previously identified binding site of E. coli ribosomal protein EL23. Therefore EL23 is likely to be the prokaryotic counterpart of L25 in spite of the limited homology displayed by the amino acid sequences of the two proteins. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/3905389/Yeast_ribosomal_protein_L25_binds_to_an_evolutionary_conserved_site_on_yeast_26S_and_E__coli_23S_rRNA_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0261-4189&date=1985&volume=4&issue=8&spage=2101 DB - PRIME DP - Unbound Medicine ER -