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Homothallic switching of Saccharomyces cerevisiae mating type genes by using a donor containing a large internal deletion.
Mol Cell Biol. 1985 Aug; 5(8):2154-8.MC

Abstract

Homothallic switching of the mating type genes of Saccharomyces cerevisiae occurs by a gene conversion event, replacing sequences at the expressed MAT locus with a DNA segment copied from one of two unexpressed loci, HML or HMR. The transposed Ya or Y alpha sequences are flanked by homologous regions that are believed to be essential for switching. We examined the transposition of a mating type gene (hmr alpha 1-delta 6) which contains a 150-base-pair deletion spanning the site where the HO endonuclease generates a double-stranded break in MAT that initiates the gene conversion event. Despite the fact that the ends of the cut MAT region no longer share homology with the donor hmr alpha 1-delta 6, switching of MATa or MAT alpha to mat alpha 1-delta 6 was efficient. However, there was a marked increase in the number of aberrant events, especially the formation of haploid-inviable fusions between MAT and the hmr alpha 1-delta 6 donor locus.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3915786

Citation

Weiffenbach, B, and J E. Haber. "Homothallic Switching of Saccharomyces Cerevisiae Mating Type Genes By Using a Donor Containing a Large Internal Deletion." Molecular and Cellular Biology, vol. 5, no. 8, 1985, pp. 2154-8.
Weiffenbach B, Haber JE. Homothallic switching of Saccharomyces cerevisiae mating type genes by using a donor containing a large internal deletion. Mol Cell Biol. 1985;5(8):2154-8.
Weiffenbach, B., & Haber, J. E. (1985). Homothallic switching of Saccharomyces cerevisiae mating type genes by using a donor containing a large internal deletion. Molecular and Cellular Biology, 5(8), 2154-8.
Weiffenbach B, Haber JE. Homothallic Switching of Saccharomyces Cerevisiae Mating Type Genes By Using a Donor Containing a Large Internal Deletion. Mol Cell Biol. 1985;5(8):2154-8. PubMed PMID: 3915786.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Homothallic switching of Saccharomyces cerevisiae mating type genes by using a donor containing a large internal deletion. AU - Weiffenbach,B, AU - Haber,J E, PY - 1985/8/1/pubmed PY - 1985/8/1/medline PY - 1985/8/1/entrez SP - 2154 EP - 8 JF - Molecular and cellular biology JO - Mol Cell Biol VL - 5 IS - 8 N2 - Homothallic switching of the mating type genes of Saccharomyces cerevisiae occurs by a gene conversion event, replacing sequences at the expressed MAT locus with a DNA segment copied from one of two unexpressed loci, HML or HMR. The transposed Ya or Y alpha sequences are flanked by homologous regions that are believed to be essential for switching. We examined the transposition of a mating type gene (hmr alpha 1-delta 6) which contains a 150-base-pair deletion spanning the site where the HO endonuclease generates a double-stranded break in MAT that initiates the gene conversion event. Despite the fact that the ends of the cut MAT region no longer share homology with the donor hmr alpha 1-delta 6, switching of MATa or MAT alpha to mat alpha 1-delta 6 was efficient. However, there was a marked increase in the number of aberrant events, especially the formation of haploid-inviable fusions between MAT and the hmr alpha 1-delta 6 donor locus. SN - 0270-7306 UR - https://www.unboundmedicine.com/medline/citation/3915786/Homothallic_switching_of_Saccharomyces_cerevisiae_mating_type_genes_by_using_a_donor_containing_a_large_internal_deletion_ L2 - https://journals.asm.org/doi/10.1128/mcb.5.8.2154-2158.1985?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -