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Morphogenesis of bacteriophage phi 29 of Bacillus subtilis: prohead restoration for DNA-gp3 packaging and assembly.
J Virol 1985; 53(3):858-61JV

Abstract

The DNA-protein complex DNA-gp3 of phi 29 is efficiently packaged into purified proheads with the aid of plasmid-derived gp16. The filled heads can be assembled to phage by addition of an extract providing the products for neck-tail assembly (Bjornsti et al., J. Virol. 50:766-772, 1984). However, purified proheads lost their competence to package DNA-gp3 upon storage for 2 months at 4 degrees C. Competence was restored by complementation with extracts of certain mutant-infected cells, and these experiments demonstrated that late proteins were not involved; restoration obtained with 4-8-14--infected cells was indistinguishable from that obtained with 7-8-14--infected cells. 2-8-14- and 3-8-14- extracts restored about one-third of the capacity to package exogenous DNA-gp3. A 1-8-14- extracts restored activity to package 20.6% of the DNA-gp3 added, but phage were not produced.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3919187

Citation

Bjornsti, M A., et al. "Morphogenesis of Bacteriophage Phi 29 of Bacillus Subtilis: Prohead Restoration for DNA-gp3 Packaging and Assembly." Journal of Virology, vol. 53, no. 3, 1985, pp. 858-61.
Bjornsti MA, Reilly BE, Anderson DL. Morphogenesis of bacteriophage phi 29 of Bacillus subtilis: prohead restoration for DNA-gp3 packaging and assembly. J Virol. 1985;53(3):858-61.
Bjornsti, M. A., Reilly, B. E., & Anderson, D. L. (1985). Morphogenesis of bacteriophage phi 29 of Bacillus subtilis: prohead restoration for DNA-gp3 packaging and assembly. Journal of Virology, 53(3), pp. 858-61.
Bjornsti MA, Reilly BE, Anderson DL. Morphogenesis of Bacteriophage Phi 29 of Bacillus Subtilis: Prohead Restoration for DNA-gp3 Packaging and Assembly. J Virol. 1985;53(3):858-61. PubMed PMID: 3919187.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morphogenesis of bacteriophage phi 29 of Bacillus subtilis: prohead restoration for DNA-gp3 packaging and assembly. AU - Bjornsti,M A, AU - Reilly,B E, AU - Anderson,D L, PY - 1985/3/1/pubmed PY - 1985/3/1/medline PY - 1985/3/1/entrez SP - 858 EP - 61 JF - Journal of virology JO - J. Virol. VL - 53 IS - 3 N2 - The DNA-protein complex DNA-gp3 of phi 29 is efficiently packaged into purified proheads with the aid of plasmid-derived gp16. The filled heads can be assembled to phage by addition of an extract providing the products for neck-tail assembly (Bjornsti et al., J. Virol. 50:766-772, 1984). However, purified proheads lost their competence to package DNA-gp3 upon storage for 2 months at 4 degrees C. Competence was restored by complementation with extracts of certain mutant-infected cells, and these experiments demonstrated that late proteins were not involved; restoration obtained with 4-8-14--infected cells was indistinguishable from that obtained with 7-8-14--infected cells. 2-8-14- and 3-8-14- extracts restored about one-third of the capacity to package exogenous DNA-gp3. A 1-8-14- extracts restored activity to package 20.6% of the DNA-gp3 added, but phage were not produced. SN - 0022-538X UR - https://www.unboundmedicine.com/medline/citation/3919187/Morphogenesis_of_bacteriophage_phi_29_of_Bacillus_subtilis:_prohead_restoration_for_DNA_gp3_packaging_and_assembly_ L2 - http://jvi.asm.org/cgi/pmidlookup?view=long&pmid=3919187 DB - PRIME DP - Unbound Medicine ER -