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Broad-host-range plasmid vector for the in vitro construction of transcriptional/translational lac fusions.
Gene 1985; 34(2-3):219-26GENE

Abstract

A broad-host-range plasmid was constructed that allows the in vitro formation of beta-galactosidase fusions. DNA from the photosynthetic bacterium Rhodopseudomonas sphaeroides was cloned into this plasmid and a number of R. sphaeroides isolates were recovered that had varying levels of beta-galactosidase activity. beta-galactosidase antigenic activity from the fusion strains could be localized immunologically in polypeptides with an Mr of 120 000 or greater. Expression of beta-galactosidase activity under control of fusion derivatives was either very low or nonexistent in Escherichia coli relative to R. sphaeroides, indicating that R. sphaeroides promoters or translational start signals function poorly in E. coli.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3924739

Citation

Nano, F E., et al. "Broad-host-range Plasmid Vector for the in Vitro Construction of Transcriptional/translational Lac Fusions." Gene, vol. 34, no. 2-3, 1985, pp. 219-26.
Nano FE, Shepherd WD, Watkins MM, et al. Broad-host-range plasmid vector for the in vitro construction of transcriptional/translational lac fusions. Gene. 1985;34(2-3):219-26.
Nano, F. E., Shepherd, W. D., Watkins, M. M., Kuhl, S. A., & Kaplan, S. (1985). Broad-host-range plasmid vector for the in vitro construction of transcriptional/translational lac fusions. Gene, 34(2-3), pp. 219-26.
Nano FE, et al. Broad-host-range Plasmid Vector for the in Vitro Construction of Transcriptional/translational Lac Fusions. Gene. 1985;34(2-3):219-26. PubMed PMID: 3924739.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Broad-host-range plasmid vector for the in vitro construction of transcriptional/translational lac fusions. AU - Nano,F E, AU - Shepherd,W D, AU - Watkins,M M, AU - Kuhl,S A, AU - Kaplan,S, PY - 1985/1/1/pubmed PY - 1985/1/1/medline PY - 1985/1/1/entrez SP - 219 EP - 26 JF - Gene JO - Gene VL - 34 IS - 2-3 N2 - A broad-host-range plasmid was constructed that allows the in vitro formation of beta-galactosidase fusions. DNA from the photosynthetic bacterium Rhodopseudomonas sphaeroides was cloned into this plasmid and a number of R. sphaeroides isolates were recovered that had varying levels of beta-galactosidase activity. beta-galactosidase antigenic activity from the fusion strains could be localized immunologically in polypeptides with an Mr of 120 000 or greater. Expression of beta-galactosidase activity under control of fusion derivatives was either very low or nonexistent in Escherichia coli relative to R. sphaeroides, indicating that R. sphaeroides promoters or translational start signals function poorly in E. coli. SN - 0378-1119 UR - https://www.unboundmedicine.com/medline/citation/3924739/Broad_host_range_plasmid_vector_for_the_in_vitro_construction_of_transcriptional/translational_lac_fusions_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0378-1119(85)90130-1 DB - PRIME DP - Unbound Medicine ER -