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[Production and standardization of Paracoccidioides brasiliensis, Histoplasma capsulatum and Aspergillus fumigatus antigens to be used in immunodiagnosis. Comparison between immunodiffusion and immunoelectrophoresis].
Mem Inst Oswaldo Cruz. 1985 Jul-Sep; 80(3):301-5.MI

Abstract

Soluble antigens (Ag) from Paracoccidioides brasiliensis, Histoplasma capsulatum and Aspergillus fumigatus were prepared and standardized by double immunodiffusion (DID) and immunoelectroosmophoresis (IEOP). No difference in sensitivity was observed between the two techniques; 100% of standard patient sera were positive with P. brasiliensis and A. fumigatus Ag and 83.3% were positive with H. capsulatum Ag. The specificity of the tests was verified testing 96 sera from patients with paracoccidioidomycosis, histoplasmosis, systemic candidiasis, sporotrichosis, tuberculosis, lung cancer, visceral or cutaneous leishmaniasis and 18 sera from healthy individuals. All the three antigens were 100% specific with the DID (using the identification pattern indicated by the confluence of test serum with standard serum precipitin lines as a positive criterium). However in the IEOP, the specificity varied with each Ag. Positive reactions with P. brasiliensis Ag were observed in 16.7% of histoplasmosis sera and in 10% of cutaneous leishmaniasis sera. On the other hand 31.8% of paracoccidioidomycosis and 10% of cutaneous leishmaniasis sera reacted with H. capsulatum Ag. The high sensitivity and specificity of the DID test, its easy reproducibility and low cost, led us to consider it highly appropriate as a routine procedure for the screening of patients with respiratory infections.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

por

PubMed ID

3939250

Citation

Ferreira-da-Cruz, M F., et al. "[Production and Standardization of Paracoccidioides Brasiliensis, Histoplasma Capsulatum and Aspergillus Fumigatus Antigens to Be Used in Immunodiagnosis. Comparison Between Immunodiffusion and Immunoelectrophoresis]." Memorias Do Instituto Oswaldo Cruz, vol. 80, no. 3, 1985, pp. 301-5.
Ferreira-da-Cruz MF, Galvão-Castro B, Wanke B. [Production and standardization of Paracoccidioides brasiliensis, Histoplasma capsulatum and Aspergillus fumigatus antigens to be used in immunodiagnosis. Comparison between immunodiffusion and immunoelectrophoresis]. Mem Inst Oswaldo Cruz. 1985;80(3):301-5.
Ferreira-da-Cruz, M. F., Galvão-Castro, B., & Wanke, B. (1985). [Production and standardization of Paracoccidioides brasiliensis, Histoplasma capsulatum and Aspergillus fumigatus antigens to be used in immunodiagnosis. Comparison between immunodiffusion and immunoelectrophoresis]. Memorias Do Instituto Oswaldo Cruz, 80(3), 301-5.
Ferreira-da-Cruz MF, Galvão-Castro B, Wanke B. [Production and Standardization of Paracoccidioides Brasiliensis, Histoplasma Capsulatum and Aspergillus Fumigatus Antigens to Be Used in Immunodiagnosis. Comparison Between Immunodiffusion and Immunoelectrophoresis]. Mem Inst Oswaldo Cruz. 1985 Jul-Sep;80(3):301-5. PubMed PMID: 3939250.
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TY - JOUR T1 - [Production and standardization of Paracoccidioides brasiliensis, Histoplasma capsulatum and Aspergillus fumigatus antigens to be used in immunodiagnosis. Comparison between immunodiffusion and immunoelectrophoresis]. AU - Ferreira-da-Cruz,M F, AU - Galvão-Castro,B, AU - Wanke,B, PY - 1985/7/1/pubmed PY - 1985/7/1/medline PY - 1985/7/1/entrez SP - 301 EP - 5 JF - Memorias do Instituto Oswaldo Cruz JO - Mem Inst Oswaldo Cruz VL - 80 IS - 3 N2 - Soluble antigens (Ag) from Paracoccidioides brasiliensis, Histoplasma capsulatum and Aspergillus fumigatus were prepared and standardized by double immunodiffusion (DID) and immunoelectroosmophoresis (IEOP). No difference in sensitivity was observed between the two techniques; 100% of standard patient sera were positive with P. brasiliensis and A. fumigatus Ag and 83.3% were positive with H. capsulatum Ag. The specificity of the tests was verified testing 96 sera from patients with paracoccidioidomycosis, histoplasmosis, systemic candidiasis, sporotrichosis, tuberculosis, lung cancer, visceral or cutaneous leishmaniasis and 18 sera from healthy individuals. All the three antigens were 100% specific with the DID (using the identification pattern indicated by the confluence of test serum with standard serum precipitin lines as a positive criterium). However in the IEOP, the specificity varied with each Ag. Positive reactions with P. brasiliensis Ag were observed in 16.7% of histoplasmosis sera and in 10% of cutaneous leishmaniasis sera. On the other hand 31.8% of paracoccidioidomycosis and 10% of cutaneous leishmaniasis sera reacted with H. capsulatum Ag. The high sensitivity and specificity of the DID test, its easy reproducibility and low cost, led us to consider it highly appropriate as a routine procedure for the screening of patients with respiratory infections. SN - 0074-0276 UR - https://www.unboundmedicine.com/medline/citation/3939250/[Production_and_standardization_of_Paracoccidioides_brasiliensis_Histoplasma_capsulatum_and_Aspergillus_fumigatus_antigens_to_be_used_in_immunodiagnosis__Comparison_between_immunodiffusion_and_immunoelectrophoresis]_ L2 - https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761985000300005&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -