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Leukotriene C4 metabolism by hepatoma cells deficient in the uptake of cysteinyl leukotrienes.
Eur J Biochem. 1986 Feb 03; 154(3):559-62.EJ

Abstract

Uptake and metabolism of the cysteinyl leukotrienes C4 and E4 (LTC4 and LTE4) were studied in AS-30D hepatoma cell suspensions and compared with rat hepatocytes. The hepatoma cells were deficient in the uptake of [3H]LTC4 and [3H]LTE4 but took up, in control experiments, L-[14C]glutamine and [14C]adenosine in a time-dependent manner. By contrast, isolated hepatocyte suspensions incubated under the same conditions took up [3H]LTC4 and [3H]LTE4 as well as L-[14C]glutamine and [14C]adenosine. The hepatoma cells deficient in the uptake of cysteinyl leukotrienes metabolized extracellular [3H]LTC4 to [3H]LTD4 and to [3H]LTE4. Addition of acivicin, an inhibitor of gamma-glutamyltransferase, largely prevented metabolism of [3H]LTC4 by the hepatoma cells. Sonication of the cells did not enhance the formation of [3H]LTD4 and [3H]LTE4 from [3H]LTC4. We conclude that ectoenzymes of AS-30D hepatoma cells catalyze the conversion of LTC4 to LTE4 via LTD4. As compared to hepatocytes, these neoplastic cells have lost the uptake system for cysteinyl leukotrienes and may serve in studies on leukotriene metabolism by cell-surface enzymes.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3948867

Citation

Weckbecker, G, and D O. Keppler. "Leukotriene C4 Metabolism By Hepatoma Cells Deficient in the Uptake of Cysteinyl Leukotrienes." European Journal of Biochemistry, vol. 154, no. 3, 1986, pp. 559-62.
Weckbecker G, Keppler DO. Leukotriene C4 metabolism by hepatoma cells deficient in the uptake of cysteinyl leukotrienes. Eur J Biochem. 1986;154(3):559-62.
Weckbecker, G., & Keppler, D. O. (1986). Leukotriene C4 metabolism by hepatoma cells deficient in the uptake of cysteinyl leukotrienes. European Journal of Biochemistry, 154(3), 559-62.
Weckbecker G, Keppler DO. Leukotriene C4 Metabolism By Hepatoma Cells Deficient in the Uptake of Cysteinyl Leukotrienes. Eur J Biochem. 1986 Feb 3;154(3):559-62. PubMed PMID: 3948867.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Leukotriene C4 metabolism by hepatoma cells deficient in the uptake of cysteinyl leukotrienes. AU - Weckbecker,G, AU - Keppler,D O, PY - 1986/2/3/pubmed PY - 1986/2/3/medline PY - 1986/2/3/entrez SP - 559 EP - 62 JF - European journal of biochemistry JO - Eur J Biochem VL - 154 IS - 3 N2 - Uptake and metabolism of the cysteinyl leukotrienes C4 and E4 (LTC4 and LTE4) were studied in AS-30D hepatoma cell suspensions and compared with rat hepatocytes. The hepatoma cells were deficient in the uptake of [3H]LTC4 and [3H]LTE4 but took up, in control experiments, L-[14C]glutamine and [14C]adenosine in a time-dependent manner. By contrast, isolated hepatocyte suspensions incubated under the same conditions took up [3H]LTC4 and [3H]LTE4 as well as L-[14C]glutamine and [14C]adenosine. The hepatoma cells deficient in the uptake of cysteinyl leukotrienes metabolized extracellular [3H]LTC4 to [3H]LTD4 and to [3H]LTE4. Addition of acivicin, an inhibitor of gamma-glutamyltransferase, largely prevented metabolism of [3H]LTC4 by the hepatoma cells. Sonication of the cells did not enhance the formation of [3H]LTD4 and [3H]LTE4 from [3H]LTC4. We conclude that ectoenzymes of AS-30D hepatoma cells catalyze the conversion of LTC4 to LTE4 via LTD4. As compared to hepatocytes, these neoplastic cells have lost the uptake system for cysteinyl leukotrienes and may serve in studies on leukotriene metabolism by cell-surface enzymes. SN - 0014-2956 UR - https://www.unboundmedicine.com/medline/citation/3948867/Leukotriene_C4_metabolism_by_hepatoma_cells_deficient_in_the_uptake_of_cysteinyl_leukotrienes_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0014-2956&date=1986&volume=154&issue=3&spage=559 DB - PRIME DP - Unbound Medicine ER -