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Lysosomal localisation of cobalamin during absorption by the ileum of the dog.
Biochim Biophys Acta. 1985 Feb 15; 838(2):206-10.BB

Abstract

The subcellular distribution of cobalamin during absorption in the dog ileum has been studied using analytical subcellular fractionation. Animals dosed orally with cyano[57Co]cobalamin were killed 2 h later, and postnuclear supernatant fractions prepared from homogenates of the ileal mucosa were subjected to isopycnic centrifugation on reorientating sucrose density gradients. Marker enzymes for the principal subcellular organelles and cyano[57Co]cobalamin were assayed in the gradient fractions. At 2 h, the distribution of cyano[57Co]cobalamin exhibited a major lysosomal localisation with only 30% of the counts being recovered in the soluble fractions. This observation was confirmed by preparing postnuclear supernatant fractions in digitonin, which selectively disrupted lysosomes and released their contents into the soluble fractions. Lysosomal localisation during passage through the ileal enterocyte strongly supports absorption of cobalamin by a process of receptor-mediated endocytosis in the dog.

Authors

Horadagoda NU
No affiliation info available
Batt RM
No affiliation info available

MeSH

AnimalsCentrifugation, IsopycnicDogsIleumIntestinal AbsorptionLysosomesTime FactorsVitamin B 12

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3970965

Citation

Horadagoda, N U., and R M. Batt. "Lysosomal Localisation of Cobalamin During Absorption By the Ileum of the Dog." Biochimica Et Biophysica Acta, vol. 838, no. 2, 1985, pp. 206-10.
Horadagoda NU, Batt RM. Lysosomal localisation of cobalamin during absorption by the ileum of the dog. Biochim Biophys Acta. 1985;838(2):206-10.
Horadagoda, N. U., & Batt, R. M. (1985). Lysosomal localisation of cobalamin during absorption by the ileum of the dog. Biochimica Et Biophysica Acta, 838(2), 206-10.
Horadagoda NU, Batt RM. Lysosomal Localisation of Cobalamin During Absorption By the Ileum of the Dog. Biochim Biophys Acta. 1985 Feb 15;838(2):206-10. PubMed PMID: 3970965.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lysosomal localisation of cobalamin during absorption by the ileum of the dog. AU - Horadagoda,N U, AU - Batt,R M, PY - 1985/2/15/pubmed PY - 1985/2/15/medline PY - 1985/2/15/entrez SP - 206 EP - 10 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 838 IS - 2 N2 - The subcellular distribution of cobalamin during absorption in the dog ileum has been studied using analytical subcellular fractionation. Animals dosed orally with cyano[57Co]cobalamin were killed 2 h later, and postnuclear supernatant fractions prepared from homogenates of the ileal mucosa were subjected to isopycnic centrifugation on reorientating sucrose density gradients. Marker enzymes for the principal subcellular organelles and cyano[57Co]cobalamin were assayed in the gradient fractions. At 2 h, the distribution of cyano[57Co]cobalamin exhibited a major lysosomal localisation with only 30% of the counts being recovered in the soluble fractions. This observation was confirmed by preparing postnuclear supernatant fractions in digitonin, which selectively disrupted lysosomes and released their contents into the soluble fractions. Lysosomal localisation during passage through the ileal enterocyte strongly supports absorption of cobalamin by a process of receptor-mediated endocytosis in the dog. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/3970965/Lysosomal_localisation_of_cobalamin_during_absorption_by_the_ileum_of_the_dog_ DB - PRIME DP - Unbound Medicine ER -